Abstract：
:Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using allografts, we established in vivo immune rejection models with induced pluripotent stem cell (iPSC)-RPE allografts and determined whether RPE-specific antibody could be detected in these models. We detected alloantibodies in the serum from recipient monkeys that had immune attacks in the retina in an immunofluorescent assay using the transplanted iPSC-RPE cells as the antigen. In addition to T cell and antigen-presenting cell immunity, peripheral blood cells and lymph nodes in animal models with allogeneic iPSC-RPE cells also had activated B cells, which were probably secreting alloantibodies. Using serum and transplanted cells, alloreactive antibody can be detected for the diagnosis of immune rejection after transplantation.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Sugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi Mdoi
10.1016/j.stemcr.2017.10.003subject
Has Abstractpub_date
2017-11-14 00:00:00pages
1501-1515issue
5issn
2213-6711pii
S2213-6711(17)30458-7journal_volume
9pub_type
杂志文章abstract：:Heterozygous mutations in the glucocerebrosidase gene (GBA) represent the strongest common genetic risk factor for Parkinson's disease (PD), the second most common neurodegenerative disorder. However, the molecular mechanisms underlying this association are still poorly understood. Here, we have analyzed ten independe...
journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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