Abstract：
:Transcriptome analysis has uncovered a series of long noncoding RNAs (lncRNAs) transcribed during cell differentiation, but how lncRNA is integrated with known transcriptional regulatory network is poorly understood. Here, we utilize human definitive endoderm differentiation as a model system and decipher the functional interaction between lncRNA and key transcriptional factor. We have identified GATA6-AS1, an lncRNA divergently transcribed from the GATA6 locus, is highly expressed during endoderm differentiation. Knockdown of GATA6-AS1 in human pluripotent stem cells has no influence on morphology and pluripotency; however, GATA6-AS1 depletion causes the deficiency of definitive endoderm differentiation. GATA6-AS1 positively regulates the expression of endoderm key factor GATA6. Further investigation shows GATA6-AS1 interacts with SMAD2/3 and activates the transcription of GATA6. In addition, overexpression of GATA6 is able to rescue the defect of endoderm differentiation due to the absence of GATA6-AS1, suggesting that GATA6 is the functional target of GATA6-AS1 during endoderm differentiation. Ultimately, our study reveals that GATA6-AS1 is necessary for human endoderm specification and reveals the underlying mechanism between GATA6-AS1 and GATA6.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Yang J,Lu P,Li M,Yan C,Zhang T,Jiang Wdoi
10.1016/j.stemcr.2020.07.014subject
Has Abstractpub_date
2020-09-08 00:00:00pages
694-705issue
3issn
2213-6711pii
S2213-6711(20)30291-5journal_volume
15pub_type
杂志文章abstract：:Insertion of reporter cassettes into the Lgr5 locus has enabled the characterization of mouse intestinal stem cells (ISCs). However, low cell surface abundance of LGR5 protein and lack of high-affinity anti-LGR5 antibodies represent a roadblock to efficiently isolate human colonic stem cells (hCoSCs). We set out to id...
journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2017.07.021
更新日期：2017-09-12 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2014.05.004
更新日期：2014-06-12 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2014.02.004
更新日期：2014-03-27 00:00:00
abstract：:Nerve-derived neural crest cells are essential for regeneration in certain animals, such as newts. Here, we asked whether they play a similar role during mammalian tissue repair, focusing on Sox2-positive neural crest precursors in skin. In adult skin, Sox2 was expressed in nerve-terminal-associated neural crest precu...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2013.04.004
更新日期：2013-06-04 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.08.011
更新日期：2018-10-09 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.03.019
更新日期：2020-05-12 00:00:00
abstract：:Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2017.10.003
更新日期：2017-11-14 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.06.008
更新日期：2020-08-11 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2013.06.001
更新日期：2013-07-11 00:00:00
abstract：:Stem Cell Reports frequently receives manuscripts dealing with the topic of cancer stem cells. Many of the submissions on this topic have major shortcomings in their content or limits to the conclusions that can be drawn from the results presented. The purpose of this Commentary is to highlight some of the underlying ...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.01.011
更新日期：2020-02-11 00:00:00
abstract：:Human induced pluripotent stem cell (hiPSC) culture has become routine, yet the cost of pluripotent cell media, frequent medium changes, and the reproducibility of differentiation have remained restrictive. Here, we describe the formulation of a hiPSC culture medium (B8) as a result of the exhaustive optimization of m...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2019.12.007
更新日期：2020-02-11 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
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更新日期：2021-01-12 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2015.09.012
更新日期：2015-11-10 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
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更新日期：2016-09-13 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2017.02.011
更新日期：2017-04-11 00:00:00
abstract：:Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nerv...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2016.05.010
更新日期：2016-06-14 00:00:00
abstract：:The balance of self-renewal and differentiation in long-term repopulating hematopoietic stem cells (LT-HSC) must be strictly controlled to maintain blood homeostasis and to prevent leukemogenesis. Hematopoietic cytokines can induce differentiation in LT-HSCs; however, the molecular mechanism orchestrating this delicat...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2014.05.010
更新日期：2014-06-19 00:00:00
abstract：:The environment surrounding stem cells has the ability to elicit profound, heritable epigenetic changes orchestrated by multiple epigenetic mechanisms, which can be modulated by the level of specific metabolites. In this review, we highlight the significance of metabolism in regulating stem cell homeostasis, cell stat...
journal_title：Stem cell reports
pub_type： 杂志文章,评审
doi：10.1016/j.stemcr.2019.09.003
更新日期：2019-10-08 00:00:00
abstract：:Pelizaeus-Merzbacher disease (PMD) is a fatal X-linked disorder caused by loss of myelinating oligodendrocytes and consequent hypomyelination. The underlying cellular and molecular dysfunctions are not fully defined, but therapeutic enhancement of oligodendrocyte survival could restore functional myelination in patien...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.07.015
更新日期：2018-09-11 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.05.010
更新日期：2020-07-14 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2019.09.006
更新日期：2019-11-12 00:00:00
abstract：:We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use of an optimized CRISPR-Cas9 system together with a double-cut donor increases homology-directed repai...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.04.013
更新日期：2018-06-05 00:00:00
