当前位置： SCI文献检索 > Stem Cell Reports期刊下所有文献 > PGC Reversion to Pluripotency Involves Erasure of DNA Methylation from Imprinting Control Centers followed by Locus-Specific Re-methylation.

PGC Reversion to Pluripotency Involves Erasure of DNA Methylation from Imprinting Control Centers followed by Locus-Specific Re-methylation.

Abstract：

:Primordial germ cells (PGCs) are fate restricted to differentiate into gametes in vivo. However, when removed from their embryonic niche, PGCs undergo reversion to pluripotent embryonic germ cells (EGCs) in vitro. One of the major differences between EGCs and embryonic stem cells (ESCs) is variable methylation at imprinting control centers (ICCs), a phenomenon that is poorly understood. Here we show that reverting PGCs to EGCs involved stable ICC methylation erasure at Snrpn, Igf2r, and Kcnqot1. In contrast, the H19/Igf2 ICC undergoes erasure followed by de novo re-methylation. PGCs differentiated in vitro from ESCs completed Snrpn ICC erasure. However, the hypomethylated state is highly unstable. We also discovered that when the H19/Igf2 ICC was abnormally hypermethylated in ESCs, this is not erased in PGCs differentiated from ESCs. Therefore, launching PGC differentiation from ESC lines with appropriately methylated ICCs is critical to the generation of germline cells that recapitulate endogenous ICC erasure.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Oliveros-Etter M,Li Z,Nee K,Hosohama L,Hargan-Calvopina J,Lee SA,Joti P,Yu J,Clark AT

doi

10.1016/j.stemcr.2015.07.006

subject

Has Abstract

pub_date

2015-09-08 00:00:00

pages

337-49

issue

3

issn

2213-6711

pii

S2213-6711(15)00212-X

journal_volume

5

pub_type

杂志文章

相关文献

Stem Cell Reports文献大全
  • BACE1 Deficiency Causes Abnormal Neuronal Clustering in the Dentate Gyrus.

    abstract：:BACE1 is validated as Alzheimer's β-secretase and a therapeutic target for Alzheimer's disease. In examining BACE1-null mice, we discovered that BACE1 deficiency develops abnormal clusters of immature neurons, forming doublecortin-positive neuroblasts, in the developing dentate gyrus, mainly in the subpial zone (SPZ)....

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.05.030

    authors： Hou H,Fan Q,He W,Suh H,Hu X,Yan R

    更新日期：2017-07-11 00:00:00

  • Human Teratoma-Derived Hematopoiesis Is a Highly Polyclonal Process Supported by Human Umbilical Vein Endothelial Cells.

    abstract：:Hematopoietic stem cells (HSCs) ensure a life-long regeneration of the blood system and are therefore an important source for transplantation and gene therapy. The teratoma environment supports the complex development of functional HSCs from human pluripotent stem cells, which is difficult to recapitulate in culture. ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.09.010

    authors： Philipp F,Selich A,Rothe M,Hoffmann D,Rittinghausen S,Morgan MA,Klatt D,Glage S,Lienenklaus S,Neuhaus V,Sewald K,Braun A,Schambach A

    更新日期：2018-11-13 00:00:00

  • Cell-type Dependent Alzheimer's Disease Phenotypes: Probing the Biology of Selective Neuronal Vulnerability.

    abstract：:Alzheimer's disease (AD) induces memory and cognitive impairment in the absence of motor and sensory deficits during its early and middle course. A major unresolved question is the basis for this selective neuronal vulnerability. Aβ, which plays a central role in AD pathogenesis, is generated throughout the brain, yet...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.10.015

    authors： Muratore CR,Zhou C,Liao M,Fernandez MA,Taylor WM,Lagomarsino VN,Pearse RV 2nd,Rice HC,Negri JM,He A,Srikanth P,Callahan DG,Shin T,Zhou M,Bennett DA,Noggle S,Love JC,Selkoe DJ,Young-Pearse TL

    更新日期：2017-12-12 00:00:00

  • In Vitro Differentiated Human Stem Cell-Derived Neurons Reproduce Synaptic Synchronicity Arising during Neurodevelopment.

    abstract：:Neurons differentiated from induced pluripotent stem cells (iPSCs) typically show regular spiking and synaptic activity but lack more complex network activity critical for brain development, such as periodic depolarizations including simultaneous involvement of glutamatergic and GABAergic neurotransmission. We generat...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.05.015

    authors： Rosa F,Dhingra A,Uysal B,Mendis GDC,Loeffler H,Elsen G,Mueller S,Schwarz N,Castillo-Lizardo M,Cuddy C,Becker F,Heutink P,Reid CA,Petrou S,Lerche H,Maljevic S

    更新日期：2020-07-14 00:00:00

  • Exercise-Induced Activated Platelets Increase Adult Hippocampal Precursor Proliferation and Promote Neuronal Differentiation.

    abstract：:Physical activity is a strong positive physiological modulator of adult neurogenesis in the hippocampal dentate gyrus. Although the underlying regulatory mechanisms are still unknown, systemic processes must be involved. Here we show that platelets are activated after acute periods of running, and that activated plate...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.02.009

    authors： Leiter O,Seidemann S,Overall RW,Ramasz B,Rund N,Schallenberg S,Grinenko T,Wielockx B,Kempermann G,Walker TL

    更新日期：2019-04-09 00:00:00

  • GATA6 Plays an Important Role in the Induction of Human Definitive Endoderm, Development of the Pancreas, and Functionality of Pancreatic β Cells.

    abstract：:Induced pluripotent stem cells were created from a pancreas agenesis patient with a mutation in GATA6. Using genome-editing technology, additional stem cell lines with mutations in both GATA6 alleles were generated and demonstrated a severe block in definitive endoderm induction, which could be rescued by re-expressio...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.12.026

    authors： Tiyaboonchai A,Cardenas-Diaz FL,Ying L,Maguire JA,Sim X,Jobaliya C,Gagne AL,Kishore S,Stanescu DE,Hughes N,De Leon DD,French DL,Gadue P

    更新日期：2017-03-14 00:00:00

  • Transcriptional Profiling of Xenogeneic Transplants: Examining Human Pluripotent Stem Cell-Derived Grafts in the Rodent Brain.

    abstract：:Human pluripotent stem cells are a valuable resource for transplantation, yet our ability to profile xenografts is largely limited to low-throughput immunohistochemical analysis by difficulties in readily isolating grafts for transcriptomic and/or proteomic profiling. Here, we present a simple methodology utilizing di...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.10.001

    authors： Bye CR,Penna V,de Luzy IR,Gantner CW,Hunt CPJ,Thompson LH,Parish CL

    更新日期：2019-11-12 00:00:00

  • Human intestinal tissue with adult stem cell properties derived from pluripotent stem cells.

    abstract：:Genetically engineered human pluripotent stem cells (hPSCs) have been proposed as a source for transplantation therapies and are rapidly becoming valuable tools for human disease modeling. However, many applications are limited due to the lack of robust differentiation paradigms that allow for the isolation of defined...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.05.001

    authors： Forster R,Chiba K,Schaeffer L,Regalado SG,Lai CS,Gao Q,Kiani S,Farin HF,Clevers H,Cost GJ,Chan A,Rebar EJ,Urnov FD,Gregory PD,Pachter L,Jaenisch R,Hockemeyer D

    更新日期：2014-06-03 00:00:00

  • Dermatopontin in Bone Marrow Extracellular Matrix Regulates Adherence but Is Dispensable for Murine Hematopoietic Cell Maintenance.

    abstract：:The hematopoietic marrow microenvironment is composed of multiple cell types embedded in an extracellular matrix (ECM). We have explored marrow ECM using mass spectrometry and found dermatopontin (DPT), a small non-collagenous ECM protein, to be present. We found that DPT cooperates with other ECM proteins to promote ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.07.021

    authors： Kramer AC,Blake AL,Taisto ME,Lehrke MJ,Webber BR,Lund TC

    更新日期：2017-09-12 00:00:00

  • Induced pluripotent stem cell generation-associated point mutations arise during the initial stages of the conversion of these cells.

    abstract：:A large number of point mutations have been identified in induced pluripotent stem cell (iPSC) genomes to date. Whether these mutations are associated with iPSC generation is an important and controversial issue. In this study, we approached this critical issue in different ways, including an assessment of iPSCs versu...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.11.006

    authors： Sugiura M,Kasama Y,Araki R,Hoki Y,Sunayama M,Uda M,Nakamura M,Ando S,Abe M

    更新日期：2014-01-02 00:00:00

  • Chemically Defined Media Can Maintain Pig Pluripotency Network In Vitro.

    abstract：:Pig embryonic stem cells (pESCs) have been considered an important candidate for preclinical research on human therapies. However, the lack of understanding of pig pluripotent networks has hampered establishment of authentic pESCs. Here, we report that FGF2, ACTVIN, and WNT signaling are essential to sustain pig pluri...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.05.028

    authors： Choi KH,Lee DK,Kim SW,Woo SH,Kim DY,Lee CK

    更新日期：2019-07-09 00:00:00

  • Injury to the spinal cord niche alters the engraftment dynamics of human neural stem cells.

    abstract：:The microenvironment is a critical mediator of stem cell survival, proliferation, migration, and differentiation. The majority of preclinical studies involving transplantation of neural stem cells (NSCs) into the CNS have focused on injured or degenerating microenvironments, leaving a dearth of information as to how N...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.03.005

    authors： Sontag CJ,Uchida N,Cummings BJ,Anderson AJ

    更新日期：2014-04-18 00:00:00

  • Reciprocal Regulation between Bifunctional miR-9/9(∗) and its Transcriptional Modulator Notch in Human Neural Stem Cell Self-Renewal and Differentiation.

    abstract：:Tight regulation of the balance between self-renewal and differentiation of neural stem cells is crucial to assure proper neural development. In this context, Notch signaling is a well-known promoter of stemness. In contrast, the bifunctional brain-enriched microRNA miR-9/9(∗) has been implicated in promoting neuronal...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.06.008

    authors： Roese-Koerner B,Stappert L,Berger T,Braun NC,Veltel M,Jungverdorben J,Evert BO,Peitz M,Borghese L,Brüstle O

    更新日期：2016-08-09 00:00:00

  • Function of Arl4aa in the Initiation of Hematopoiesis in Zebrafish by Maintaining Golgi Complex Integrity in Hemogenic Endothelium.

    abstract：:ADP-ribosylation factor-like 4aa (Arl4aa) is a member of the ADP-ribosylation factor family. It is expressed in hematopoietic tissue during embryonic development, but its function was unknown. Zebrafish arl4aa is preferentially expressed in the ventral wall of the dorsal aorta (VDA) at 24 and 36 hpf and in caudal hema...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.02.012

    authors： Guo Y,Cheng BYL,Wang D,Ma ACH,He BL,Man TK,Cheung MPL,Shi X,Ng NKL,Leung AYH

    更新日期：2020-04-14 00:00:00

  • GDNF/GFRα1 Complex Abrogates Self-Renewing Activity of Cortical Neural Precursors Inducing Their Differentiation.

    abstract：:The balance between factors leading to proliferation and differentiation of cortical neural precursors (CNPs) determines the correct cortical development. In this work, we show that GDNF and its receptor GFRα1 are expressed in the neocortex during the period of cortical neurogenesis. We show that the GDNF/GFRα1 comple...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.01.019

    authors： Bonafina A,Fontanet PA,Paratcha G,Ledda F

    更新日期：2018-03-13 00:00:00

  • BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.

    abstract：:B-lymphocyte-induced nuclear maturation protein 1 (BLIMP1) was previously reported to define a sebaceous gland (SG) progenitor population in the epidermis. However, the recent identification of multiple stem cell populations in the hair follicle junctional zone has led us to re-evaluate its function. We show, in agree...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.08.007

    authors： Kretzschmar K,Cottle DL,Donati G,Chiang MF,Quist SR,Gollnick HP,Natsuga K,Lin KI,Watt FM

    更新日期：2014-10-14 00:00:00

  • Pluripotent Stem Cell Model of Nakajo-Nishimura Syndrome Untangles Proinflammatory Pathways Mediated by Oxidative Stress.

    abstract：:Nakajo-Nishimura syndrome (NNS) is an immunoproteasome-associated autoinflammatory disorder caused by a mutation of the PSMB8 gene. Although dysfunction of the immunoproteasome causes various cellular stresses attributed to the overproduction of inflammatory cytokines and chemokines in NNS, the underlying mechanisms o...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.04.004

    authors： Honda-Ozaki F,Terashima M,Niwa A,Saiki N,Kawasaki Y,Ito H,Hotta A,Nagahashi A,Igura K,Asaka I,Li HL,Yanagimachi M,Furukawa F,Kanazawa N,Nakahata T,Saito MK

    更新日期：2018-06-05 00:00:00

  • Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and Functionality.

    abstract：:Emerging evidence suggests that Parkinson's disease (PD), besides being an age-associated disorder, might also have a neurodevelopment component. Disruption of mitochondrial homeostasis has been highlighted as a crucial cofactor in its etiology. Here, we show that PD patient-specific human neuroepithelial stem cells (...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.03.004

    authors： Walter J,Bolognin S,Antony PMA,Nickels SL,Poovathingal SK,Salamanca L,Magni S,Perfeito R,Hoel F,Qing X,Jarazo J,Arias-Fuenzalida J,Ignac T,Monzel AS,Gonzalez-Cano L,Pereira de Almeida L,Skupin A,Tronstad KJ,Schwamborn

    更新日期：2019-05-14 00:00:00

  • In Vivo Generation of Engraftable Murine Hematopoietic Stem Cells by Gfi1b, c-Fos, and Gata2 Overexpression within Teratoma.

    abstract：:Generation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) could potentially provide unlimited HSCs for clinical transplantation, a curative treatment for numerous blood diseases. However, to date, bona fide HSC generation has been largely unsuccessful in vitro. We have previously described proof...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.08.010

    authors： Tsukada M,Ota Y,Wilkinson AC,Becker HJ,Osato M,Nakauchi H,Yamazaki S

    更新日期：2017-10-10 00:00:00

  • The case for stem cell counselors.

    abstract：:In this article, Scott describes how a new counseling profession could support clinical sites and patients enrolling in stem cell clinical trials. A possible model is proposed, along with a curriculum that would provide counselors with the tools to address challenges facing the clinical stem cell field. Finally, a can...

    journal_title：Stem cell reports

    pub_type： 信件

    doi：10.1016/j.stemcr.2014.10.016

    authors： Scott CT

    更新日期：2015-01-13 00:00:00

  • PGC-1α and reactive oxygen species regulate human embryonic stem cell-derived cardiomyocyte function.

    abstract：:Diminished mitochondrial function is causally related to some heart diseases. Here, we developed a human disease model based on cardiomyocytes from human embryonic stem cells (hESCs), in which an important pathway of mitochondrial gene expression was inactivated. Repression of PGC-1α, which is normally induced during ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.11.008

    authors： Birket MJ,Casini S,Kosmidis G,Elliott DA,Gerencser AA,Baartscheer A,Schumacher C,Mastroberardino PG,Elefanty AG,Stanley EG,Mummery CL

    更新日期：2013-12-12 00:00:00

  • Isolation of Human Colon Stem Cells Using Surface Expression of PTK7.

    abstract：:Insertion of reporter cassettes into the Lgr5 locus has enabled the characterization of mouse intestinal stem cells (ISCs). However, low cell surface abundance of LGR5 protein and lack of high-affinity anti-LGR5 antibodies represent a roadblock to efficiently isolate human colonic stem cells (hCoSCs). We set out to id...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.10.003

    authors： Jung P,Sommer C,Barriga FM,Buczacki SJ,Hernando-Momblona X,Sevillano M,Duran-Frigola M,Aloy P,Selbach M,Winton DJ,Batlle E

    更新日期：2015-12-08 00:00:00

  • ISSCR 2013: back to Bean Town.

    abstract：:The International Society for Stem Cell Research 11(th) Annual Meeting was held in Boston in June 2013, bringing together just over 4000 attendees. An emphasis on therapeutic applications in many talks reflected the maturation of the stem cell field from its origins in basic science to one that is beginning to show th...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.12.004

    authors： Brack AS,Hochedlinger K

    更新日期：2013-01-01 00:00:00

  • Human embryonic and induced pluripotent stem cell research trends: complementation and diversification of the field.

    abstract：:Research in human induced pluripotent stem cells (hiPSCs) is rapidly developing and there are expectations that this research may obviate the need to use human embryonic stem cells (hESCs), the ethics of which has been a subject of controversy for more than 15 years. In this study, we investigated approximately 3,400 ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.03.002

    authors： Kobold S,Guhr A,Kurtz A,Löser P

    更新日期：2015-05-12 00:00:00

  • iPSC-MSCs with High Intrinsic MIRO1 and Sensitivity to TNF-α Yield Efficacious Mitochondrial Transfer to Rescue Anthracycline-Induced Cardiomyopathy.

    abstract：:Mesenchymal stem cells (MSCs) can donate mitochondria and rescue anthracycline-induced cardiomyocyte (CM) damage, although the underlying mechanisms remain elusive. We determined that the superior efficiency of mitochondrial transfer by human induced-pluripotent-stem-cell-derived MSCs (iPSC-MSCs) compared with bone ma...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.08.009

    authors： Zhang Y,Yu Z,Jiang D,Liang X,Liao S,Zhang Z,Yue W,Li X,Chiu SM,Chai YH,Liang Y,Chow Y,Han S,Xu A,Tse HF,Lian Q

    更新日期：2016-10-11 00:00:00

  • Cancer Stem Cells: Notes for Authors.

    abstract：:Stem Cell Reports frequently receives manuscripts dealing with the topic of cancer stem cells. Many of the submissions on this topic have major shortcomings in their content or limits to the conclusions that can be drawn from the results presented. The purpose of this Commentary is to highlight some of the underlying ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.01.011

    authors： Eaves CJ,Pera MF

    更新日期：2020-02-11 00:00:00

  • Detecting Genetic Mosaicism in Cultures of Human Pluripotent Stem Cells.

    abstract：:Genetic changes in human pluripotent stem cells (hPSCs) gained during culture can confound experimental results and potentially jeopardize the outcome of clinical therapies. Particularly common changes in hPSCs are trisomies of chromosomes 1, 12, 17, and 20. Thus, hPSCs should be regularly screened for such aberration...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.10.003

    authors： Baker D,Hirst AJ,Gokhale PJ,Juarez MA,Williams S,Wheeler M,Bean K,Allison TF,Moore HD,Andrews PW,Barbaric I

    更新日期：2016-11-08 00:00:00

  • Enhanced Energetic State and Protection from Oxidative Stress in Human Myoblasts Overexpressing BMI1.

    abstract：:The Polycomb group gene BMI1 is essential for efficient muscle regeneration in a mouse model of Duchenne muscular dystrophy, and its enhanced expression in adult skeletal muscle satellite cells ameliorates the muscle strength in this model. Here, we show that the impact of mild BMI1 overexpression observed in mouse mo...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.06.009

    authors： Dibenedetto S,Niklison-Chirou M,Cabrera CP,Ellis M,Robson LG,Knopp P,Tedesco FS,Ragazzi M,Di Foggia V,Barnes MR,Radunovic A,Marino S

    更新日期：2017-08-08 00:00:00

  • Maternal inflammation contributes to brain overgrowth and autism-associated behaviors through altered redox signaling in stem and progenitor cells.

    abstract：:A period of mild brain overgrowth with an unknown etiology has been identified as one of the most common phenotypes in autism. Here, we test the hypothesis that maternal inflammation during critical periods of embryonic development can cause brain overgrowth and autism-associated behaviors as a result of altered neura...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.09.004

    authors： Le Belle JE,Sperry J,Ngo A,Ghochani Y,Laks DR,López-Aranda M,Silva AJ,Kornblum HI

    更新日期：2014-11-11 00:00:00

  • Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development.

    abstract：:Human stem cell-derived models of development and neurodegenerative diseases are challenged by cellular immaturity in vitro. Microengineered organ-on-chip (or Organ-Chip) systems are designed to emulate microvolume cytoarchitecture and enable co-culture of distinct cell types. Brain microvascular endothelial cells (BM...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.02.012

    authors： Sances S,Ho R,Vatine G,West D,Laperle A,Meyer A,Godoy M,Kay PS,Mandefro B,Hatata S,Hinojosa C,Wen N,Sareen D,Hamilton GA,Svendsen CN

    更新日期：2018-04-10 00:00:00