Abstract：
:Pig embryonic stem cells (pESCs) have been considered an important candidate for preclinical research on human therapies. However, the lack of understanding of pig pluripotent networks has hampered establishment of authentic pESCs. Here, we report that FGF2, ACTVIN, and WNT signaling are essential to sustain pig pluripotency in vitro. Newly derived pESCs were stably maintained over an extended period, and capable of forming teratomas that contained three germ layers. Transcriptome analysis showed that pESCs were developmentally similar to late epiblasts of preimplantation embryos and in terms of biological functions resembled human rather than mouse pluripotent stem cells. However, the pESCs had distinct features such as coexpression of SSEA1 and SSEA4, two active X chromosomes, and a unique transcriptional pattern. Our findings will facilitate both the development of large animal models for human stem cell therapy and the generation of pluripotent stem cells from other domestic animals for agricultural use.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Choi KH,Lee DK,Kim SW,Woo SH,Kim DY,Lee CKdoi
10.1016/j.stemcr.2019.05.028subject
Has Abstractpub_date
2019-07-09 00:00:00pages
221-234issue
1issn
2213-6711pii
S2213-6711(19)30196-1journal_volume
13pub_type
杂志文章abstract：:Self-renewal potential and multipotency are hallmarks of a stem cell. It is generally accepted that acquisition of such stemness requires rejuvenation of somatic cells through reprogramming of their genetic and epigenetic status.We show here that a simple block of cell differentiation is sufficient to induce and maint...
journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
pub_type： 杂志文章
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abstract：:Interleukin-6 (IL-6) is increased in maternal serum and amniotic fluid of children subsequently diagnosed with autism spectrum disorders. However, it is not clear how increased IL-6 alters brain development. Here, we show that IL-6 increases the prevalence of a specific platelet-derived growth factor (PDGF)-responsive...
journal_title：Stem cell reports
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journal_title：Stem cell reports
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doi：10.1016/j.stemcr.2018.01.019
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.08.018
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abstract：:Research in human induced pluripotent stem cells (hiPSCs) is rapidly developing and there are expectations that this research may obviate the need to use human embryonic stem cells (hESCs), the ethics of which has been a subject of controversy for more than 15 years. In this study, we investigated approximately 3,400 ...
journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
pub_type： 杂志文章
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abstract：:The small intestine plays an important role in the absorption and metabolism of oral drugs. In the current evaluation system, it is difficult to predict the precise absorption and metabolism of oral drugs. In this study, we generated small intestinal epithelial-like cells from human induced pluripotent stem cells (hiP...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.10.019
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