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Deriving Dorsal Spinal Sensory Interneurons from Human Pluripotent Stem Cells.

Abstract：

:Cellular replacement therapies for neurological conditions use human embryonic stem cell (hESC)- or induced pluripotent stem cell (hiPSC)-derived neurons to replace damaged or diseased populations of neurons. For the spinal cord, significant progress has been made generating the in-vitro-derived motor neurons required to restore coordinated movement. However, there is as yet no protocol to generate in-vitro-derived sensory interneurons (INs), which permit perception of the environment. Here, we report on the development of a directed differentiation protocol to derive sensory INs for both hESCs and hiPSCs. Two developmentally relevant factors, retinoic acid in combination with bone morphogenetic protein 4, can be used to generate three classes of sensory INs: the proprioceptive dI1s, the dI2s, and mechanosensory dI3s. Critical to this protocol is the competence state of the neural progenitors, which changes over time. This protocol will facilitate developing cellular replacement therapies to reestablish sensory connections in injured patients.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Gupta S,Sivalingam D,Hain S,Makkar C,Sosa E,Clark A,Butler SJ

doi

10.1016/j.stemcr.2017.12.012

subject

Has Abstract

pub_date

2018-02-13 00:00:00

pages

390-405

issue

2

issn

2213-6711

pii

S2213-6711(17)30560-X

journal_volume

10

pub_type

杂志文章

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