当前位置： SCI文献检索 > Stem Cell Reports期刊下所有文献 > Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid.

Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid.

Abstract：

:In this study we dissected retinal organoid morphogenesis in human embryonic stem cell (hESC)-derived cultures and established a convenient method for isolating large quantities of retinal organoids for modeling human retinal development and disease. Epithelialized cysts were generated via floating culture of clumps of Matrigel/hESCs. Upon spontaneous attachment and spreading of the cysts, patterned retinal monolayers with tight junctions formed. Dispase-mediated detachment of the monolayers and subsequent floating culture led to self-formation of retinal organoids comprising patterned neuroretina, ciliary margin, and retinal pigment epithelium. Intercellular adhesion-dependent cell survival and ROCK-regulated actomyosin-driven forces are required for the self-organization. Our data supports a hypothesis that newly specified neuroretina progenitors form characteristic structures in equilibrium through minimization of cell surface tension. In long-term culture, the retinal organoids autonomously generated stratified retinal tissues, including photoreceptors with ultrastructure of outer segments. Our system requires minimal manual manipulation, has been validated in two lines of human pluripotent stem cells, and provides insight into optic cup invagination in vivo.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Lowe A,Harris R,Bhansali P,Cvekl A,Liu W

doi

10.1016/j.stemcr.2016.03.011

subject

Has Abstract

pub_date

2016-05-10 00:00:00

pages

743-756

issue

5

issn

2213-6711

pii

S2213-6711(16)30006-6

journal_volume

6

pub_type

杂志文章

相关文献

Stem Cell Reports文献大全
  • Integrative Analysis of the Acquisition of Pluripotency in PGCs Reveals the Mutually Exclusive Roles of Blimp-1 and AKT Signaling.

    abstract：:Primordial germ cells (PGCs) are lineage-restricted unipotent cells that can dedifferentiate into pluripotent embryonic germ cells (EGCs). Here we performed whole-transcriptome analysis during the conversion of PGCs into EGCs, a process by which cells acquire pluripotency. To examine the molecular mechanism underlying...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.05.007

    authors： Nagamatsu G,Saito S,Takubo K,Suda T

    更新日期：2015-07-14 00:00:00

  • Modeling Developmental and Tumorigenic Aspects of Trilateral Retinoblastoma via Human Embryonic Stem Cells.

    abstract：:Human embryonic stem cells (hESCs) provide a platform for studying human development and understanding mechanisms underlying diseases. Retinoblastoma-1 (RB1) is a key regulator of cell cycling, of which biallelic inactivation initiates retinoblastoma, the most common congenital intraocular malignancy. We developed a m...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.03.005

    authors： Avior Y,Lezmi E,Yanuka D,Benvenisty N

    更新日期：2017-05-09 00:00:00

  • Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and Functionality.

    abstract：:Emerging evidence suggests that Parkinson's disease (PD), besides being an age-associated disorder, might also have a neurodevelopment component. Disruption of mitochondrial homeostasis has been highlighted as a crucial cofactor in its etiology. Here, we show that PD patient-specific human neuroepithelial stem cells (...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.03.004

    authors： Walter J,Bolognin S,Antony PMA,Nickels SL,Poovathingal SK,Salamanca L,Magni S,Perfeito R,Hoel F,Qing X,Jarazo J,Arias-Fuenzalida J,Ignac T,Monzel AS,Gonzalez-Cano L,Pereira de Almeida L,Skupin A,Tronstad KJ,Schwamborn

    更新日期：2019-05-14 00:00:00

  • Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System.

    abstract：:Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nerv...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.05.010

    authors： Rigamonti A,Repetti GG,Sun C,Price FD,Reny DC,Rapino F,Weisinger K,Benkler C,Peterson QP,Davidow LS,Hansson EM,Rubin LL

    更新日期：2016-06-14 00:00:00

  • Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner.

    abstract：:Human vision relies heavily upon cone photoreceptors, and their loss results in permanent visual impairment. Transplantation of healthy photoreceptors can restore visual function in models of inherited blindness, a process previously understood to arise by donor cell integration within the host retina. However, we and...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.12.008

    authors： Waldron PV,Di Marco F,Kruczek K,Ribeiro J,Graca AB,Hippert C,Aghaizu ND,Kalargyrou AA,Barber AC,Grimaldi G,Duran Y,Blackford SJI,Kloc M,Goh D,Zabala Aldunate E,Sampson RD,Bainbridge JWB,Smith AJ,Gonzalez-Cordero A,S

    更新日期：2018-02-13 00:00:00

  • Microscale generation of cardiospheres promotes robust enrichment of cardiomyocytes derived from human pluripotent stem cells.

    abstract：:Cardiomyocytes derived from human pluripotent stem cells (hPSCs) are a promising cell source for regenerative medicine, disease modeling, and drug discovery, all of which require enriched cardiomyocytes, ideally ones with mature phenotypes. However, current methods are typically performed in 2D environments that produ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.06.002

    authors： Nguyen DC,Hookway TA,Wu Q,Jha R,Preininger MK,Chen X,Easley CA,Spearman P,Deshpande SR,Maher K,Wagner MB,McDevitt TC,Xu C

    更新日期：2014-08-12 00:00:00

  • Epigenetic rejuvenation of mesenchymal stromal cells derived from induced pluripotent stem cells.

    abstract：:Standardization of mesenchymal stromal cells (MSCs) remains a major obstacle in regenerative medicine. Starting material and culture expansion affect cell preparations and render comparison between studies difficult. In contrast, induced pluripotent stem cells (iPSCs) assimilate toward a ground state and may therefore...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.07.003

    authors： Frobel J,Hemeda H,Lenz M,Abagnale G,Joussen S,Denecke B,Sarić T,Zenke M,Wagner W

    更新日期：2014-09-09 00:00:00

  • Sox2-mediated regulation of adult neural crest precursors and skin repair.

    abstract：:Nerve-derived neural crest cells are essential for regeneration in certain animals, such as newts. Here, we asked whether they play a similar role during mammalian tissue repair, focusing on Sox2-positive neural crest precursors in skin. In adult skin, Sox2 was expressed in nerve-terminal-associated neural crest precu...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.04.004

    authors： Johnston AP,Naska S,Jones K,Jinno H,Kaplan DR,Miller FD

    更新日期：2013-06-04 00:00:00

  • A method to identify and isolate pluripotent human stem cells and mouse epiblast stem cells using lipid body-associated retinyl ester fluorescence.

    abstract：:We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450-500 nm) is readily observed by fluorescence microscopy and correlates with the expression of pluripotency markers (OCT4,...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.05.004

    authors： Muthusamy T,Mukherjee O,Menon R,Megha PB,Panicker MM

    更新日期：2014-06-12 00:00:00

  • Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming.

    abstract：:Reprogramming of somatic cells produces induced pluripotent stem cells (iPSCs) that are invaluable resources for biomedical research. Here, we extended the previous transcriptome studies by performing RNA-seq on cells defined by a combination of multiple cellular surface markers. We found that transcriptome changes du...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.04.009

    authors： Tanaka Y,Hysolli E,Su J,Xiang Y,Kim KY,Zhong M,Li Y,Heydari K,Euskirchen G,Snyder MP,Pan X,Weissman SM,Park IH

    更新日期：2015-06-09 00:00:00

  • Enhanced Reconstitution of Human Erythropoiesis and Thrombopoiesis in an Immunodeficient Mouse Model with Kit(Wv) Mutations.

    abstract：:In human-to-mouse xenograft models, reconstitution of human hematopoiesis is usually B-lymphoid dominant. Here we show that the introduction of homozygous Kit(Wv) mutations into C57BL/6.Rag2(null)Il2rg(null) mice with NOD-Sirpa (BRGS) strongly promoted human multi-lineage reconstitution. After xenotransplantation of h...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.07.002

    authors： Yurino A,Takenaka K,Yamauchi T,Nunomura T,Uehara Y,Jinnouchi F,Miyawaki K,Kikushige Y,Kato K,Miyamoto T,Iwasaki H,Kunisaki Y,Akashi K

    更新日期：2016-09-13 00:00:00

  • The epithelial-mesenchymal transition factor SNAIL paradoxically enhances reprogramming.

    abstract：:Reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) entails a mesenchymal to epithelial transition (MET). While attempting to dissect the mechanism of MET during reprogramming, we observed that knockdown (KD) of the epithelial-to-mesenchymal transition (EMT) factor SNAI1 (SNAIL) paradoxically reduce...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.09.008

    authors： Unternaehrer JJ,Zhao R,Kim K,Cesana M,Powers JT,Ratanasirintrawoot S,Onder T,Shibue T,Weinberg RA,Daley GQ

    更新日期：2014-11-11 00:00:00

  • Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein.

    abstract：:Spermatogonial stem cells (SSCs) serve as a resource for producing genetically modified animals. However, genetic manipulation of SSCs has met with limited success. Here, we show efficient gene transfer into SSCs via a lentivirus (FV-LV) using a fusion protein (F), a Sendai virus (SV) envelope protein involved in viri...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.02.001

    authors： Shinohara T,Kanatsu-Shinohara M

    更新日期：2020-03-10 00:00:00

  • Phenotypic Screening Identifies Modulators of Amyloid Precursor Protein Processing in Human Stem Cell Models of Alzheimer's Disease.

    abstract：:Human stem cell models have the potential to provide platforms for phenotypic screens to identify candidate treatments and cellular pathways involved in the pathogenesis of neurodegenerative disorders. Amyloid precursor protein (APP) processing and the accumulation of APP-derived amyloid β (Aβ) peptides are key proces...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.02.006

    authors： Brownjohn PW,Smith J,Portelius E,Serneels L,Kvartsberg H,De Strooper B,Blennow K,Zetterberg H,Livesey FJ

    更新日期：2017-04-11 00:00:00

  • Inhibition of Gata4 and Tbx5 by Nicotine-Mediated DNA Methylation in Myocardial Differentiation.

    abstract：:Maternal nicotine exposure causes alteration of gene expression and cardiovascular programming. The discovery of nicotine-medicated regulation in cardiogenesis is of major importance for the study of cardiac defects. The present study investigated the effect of nicotine on cardiac gene expression and epigenetic regula...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.12.016

    authors： Jiang XY,Feng YL,Ye LT,Li XH,Feng J,Zhang MZ,Shelat HS,Wassler M,Li Y,Geng YJ,Yu XY

    更新日期：2017-02-14 00:00:00

  • Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes.

    abstract：:Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 106 hiPSCs per layer yiel...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.08.025

    authors： Tohyama S,Fujita J,Fujita C,Yamaguchi M,Kanaami S,Ohno R,Sakamoto K,Kodama M,Kurokawa J,Kanazawa H,Seki T,Kishino Y,Okada M,Nakajima K,Tanosaki S,Someya S,Hirano A,Kawaguchi S,Kobayashi E,Fukuda K

    更新日期：2017-11-14 00:00:00

  • A Standard Nomenclature for Referencing and Authentication of Pluripotent Stem Cells.

    abstract：:Unambiguous cell line authentication is essential to avoid loss of association between data and cells. The risk for loss of references increases with the rapidity that new human pluripotent stem cell (hPSC) lines are generated, exchanged, and implemented. Ideally, a single name should be used as a generally applied re...

    journal_title：Stem cell reports

    pub_type： 杂志文章,评审

    doi：10.1016/j.stemcr.2017.12.002

    authors： Kurtz A,Seltmann S,Bairoch A,Bittner MS,Bruce K,Capes-Davis A,Clarke L,Crook JM,Daheron L,Dewender J,Faulconbridge A,Fujibuchi W,Gutteridge A,Hei DJ,Kim YO,Kim JH,Kokocinski AK,Lekschas F,Lomax GP,Loring JF,Ludwig

    更新日期：2018-01-09 00:00:00

  • Studies in an Early Development Window Unveils a Severe HSC Defect in both Murine and Human Fanconi Anemia.

    abstract：:Fanconi anemia (FA) causes bone marrow failure early during childhood, and recent studies indicate that a hematopoietic defect could begin in utero. We performed a unique kinetics study of hematopoiesis in Fancg-/- mouse embryos, between the early embryonic day 11.5 (E11.5) to E12.5 developmental window (when the high...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.10.001

    authors： Domenech C,Maillard L,Rousseau A,Guidez F,Petit L,Pla M,Clay D,Guimiot F,Sanfilippo S,Jacques S,de la Grange P,Robil N,Soulier J,Souyri M

    更新日期：2018-11-13 00:00:00

  • A Comprehensive Resource for Induced Pluripotent Stem Cells from Patients with Primary Tauopathies.

    abstract：:Primary tauopathies are characterized neuropathologically by inclusions containing abnormal forms of the microtubule-associated protein tau (MAPT) and clinically by diverse neuropsychiatric, cognitive, and motor impairments. Autosomal dominant mutations in the MAPT gene cause heterogeneous forms of frontotemporal loba...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.09.006

    authors： Karch CM,Kao AW,Karydas A,Onanuga K,Martinez R,Argouarch A,Wang C,Huang C,Sohn PD,Bowles KR,Spina S,Silva MC,Marsh JA,Hsu S,Pugh DA,Ghoshal N,Norton J,Huang Y,Lee SE,Seeley WW,Theofilas P,Grinberg LT,Moreno F

    更新日期：2019-11-12 00:00:00

  • ADAM8 Is an Antigen of Tyrosine Kinase Inhibitor-Resistant Chronic Myeloid Leukemia Cells Identified by Patient-Derived Induced Pluripotent Stem Cells.

    abstract：:Properties of cancer stem cells involved in drug resistance and relapse have significant effects on clinical outcome. Although tyrosine kinase inhibitors (TKIs) have dramatically improved survival of patients with chronic myeloid leukemia (CML), TKIs have not fully cured CML due to TKI-resistant CML stem cells. Moreov...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.01.015

    authors： Miyauchi M,Koya J,Arai S,Yamazaki S,Honda A,Kataoka K,Yoshimi A,Taoka K,Kumano K,Kurokawa M

    更新日期：2018-03-13 00:00:00

  • Rapid Crypt Cell Remodeling Regenerates the Intestinal Stem Cell Niche after Notch Inhibition.

    abstract：:Intestinal crypts have great capacity for repair and regeneration after intestinal stem cell (ISC) injury. Here, we define the cellular remodeling process resulting from ISC niche interruption by transient Notch pathway inhibition in adult mice. Although ISCs were retained, lineage tracing demonstrated a marked reduct...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.05.010

    authors： Bohin N,Keeley TM,Carulli AJ,Walker EM,Carlson EA,Gao J,Aifantis I,Siebel CW,Rajala MW,Myers MG Jr,Jones JC,Brindley CD,Dempsey PJ,Samuelson LC

    更新日期：2020-07-14 00:00:00

  • Integrated Genomic Analysis of Diverse Induced Pluripotent Stem Cells from the Progenitor Cell Biology Consortium.

    abstract：:The rigorous characterization of distinct induced pluripotent stem cells (iPSC) derived from multiple reprogramming technologies, somatic sources, and donors is required to understand potential sources of variability and downstream potential. To achieve this goal, the Progenitor Cell Biology Consortium performed compr...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.05.006

    authors： Salomonis N,Dexheimer PJ,Omberg L,Schroll R,Bush S,Huo J,Schriml L,Ho Sui S,Keddache M,Mayhew C,Shanmukhappa SK,Wells J,Daily K,Hubler S,Wang Y,Zambidis E,Margolin A,Hide W,Hatzopoulos AK,Malik P,Cancelas JA,Aro

    更新日期：2016-07-12 00:00:00

  • Pathological ASXL1 Mutations and Protein Variants Impair Neural Crest Development.

    abstract：:The neural crest (NC) gives rise to a multitude of fetal tissues, and its misregulation is implicated in congenital malformations. Here, we investigated molecular mechanisms pertaining to NC-related symptoms in Bohring-Opitz syndrome (BOS), a developmental disorder linked to mutations in the Polycomb group factor Addi...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.03.006

    authors： Matheus F,Rusha E,Rehimi R,Molitor L,Pertek A,Modic M,Feederle R,Flatley A,Kremmer E,Geerlof A,Rishko V,Rada-Iglesias A,Drukker M

    更新日期：2019-05-14 00:00:00

  • Injury to the spinal cord niche alters the engraftment dynamics of human neural stem cells.

    abstract：:The microenvironment is a critical mediator of stem cell survival, proliferation, migration, and differentiation. The majority of preclinical studies involving transplantation of neural stem cells (NSCs) into the CNS have focused on injured or degenerating microenvironments, leaving a dearth of information as to how N...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.03.005

    authors： Sontag CJ,Uchida N,Cummings BJ,Anderson AJ

    更新日期：2014-04-18 00:00:00

  • Generation of foxn1/Casper Mutant Zebrafish for Allograft and Xenograft of Normal and Malignant Cells.

    abstract：:Cell transplantation into immunodeficient recipients is a widely used approach to study stem cell and cancer biology; however, studying cell states post transplantation in vivo is inconvenient in mammals. Here, we generated a foxn1/Casper mutant zebrafish that is transparent and exhibits T cell deficiency. By employin...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.07.020

    authors： Lv P,Ma D,Gao S,Zhang Y,Bae YK,Liang G,Gao S,Choi JH,Kim CH,Wang L,Liu F

    更新日期：2020-09-08 00:00:00

  • BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions.

    abstract：:B-lymphocyte-induced nuclear maturation protein 1 (BLIMP1) was previously reported to define a sebaceous gland (SG) progenitor population in the epidermis. However, the recent identification of multiple stem cell populations in the hair follicle junctional zone has led us to re-evaluate its function. We show, in agree...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.08.007

    authors： Kretzschmar K,Cottle DL,Donati G,Chiang MF,Quist SR,Gollnick HP,Natsuga K,Lin KI,Watt FM

    更新日期：2014-10-14 00:00:00

  • ISSCR 2013: back to Bean Town.

    abstract：:The International Society for Stem Cell Research 11(th) Annual Meeting was held in Boston in June 2013, bringing together just over 4000 attendees. An emphasis on therapeutic applications in many talks reflected the maturation of the stem cell field from its origins in basic science to one that is beginning to show th...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.12.004

    authors： Brack AS,Hochedlinger K

    更新日期：2013-01-01 00:00:00

  • Sox100B Regulates Progenitor-Specific Gene Expression and Cell Differentiation in the Adult Drosophila Intestine.

    abstract：:Robust production of terminally differentiated cells from self-renewing resident stem cells is essential to maintain proper tissue architecture and physiological functions, especially in high-turnover tissues. However, the transcriptional networks that precisely regulate cell transition and differentiation are poorly ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.01.003

    authors： Meng FW,Rojas Villa SE,Biteau B

    更新日期：2020-02-11 00:00:00

  • Induced pluripotent stem cell generation-associated point mutations arise during the initial stages of the conversion of these cells.

    abstract：:A large number of point mutations have been identified in induced pluripotent stem cell (iPSC) genomes to date. Whether these mutations are associated with iPSC generation is an important and controversial issue. In this study, we approached this critical issue in different ways, including an assessment of iPSCs versu...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.11.006

    authors： Sugiura M,Kasama Y,Araki R,Hoki Y,Sunayama M,Uda M,Nakamura M,Ando S,Abe M

    更新日期：2014-01-02 00:00:00

  • Early depletion of primordial germ cells in zebrafish promotes testis formation.

    abstract：:As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation. We applied morpholin...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.10.011

    authors： Tzung KW,Goto R,Saju JM,Sreenivasan R,Saito T,Arai K,Yamaha E,Hossain MS,Calvert MEK,Orbán L

    更新日期：2015-01-13 00:00:00