Abstract:
:Human embryonic stem cells (hESCs) provide a platform for studying human development and understanding mechanisms underlying diseases. Retinoblastoma-1 (RB1) is a key regulator of cell cycling, of which biallelic inactivation initiates retinoblastoma, the most common congenital intraocular malignancy. We developed a model to study the role of RB1 in early development and tumor formation by generating RB1-null hESCs using CRISPR/Cas9. RB1-/- hESCs initiated extremely large teratomas, with neural expansions similar to those of trilateral retinoblastoma tumors, in which retinoblastoma is accompanied by intracranial neural tumors. Teratoma analysis further revealed a role for the transcription factor ZEB1 in RB1-mediated ectoderm differentiation. Furthermore, RB1-/- cells displayed mitochondrial dysfunction similar to poorly differentiated retinoblastomas. Screening more than 100 chemotherapies revealed an RB1-/--specific cell sensitivity to carboplatin, exploiting their mitochondrial dysfunction. Together, our work provides a human pluripotent cell model for retinoblastoma and sheds light on developmental and tumorigenic roles of RB1.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Avior Y,Lezmi E,Yanuka D,Benvenisty Ndoi
10.1016/j.stemcr.2017.03.005subject
Has Abstractpub_date
2017-05-09 00:00:00pages
1354-1365issue
5issn
2213-6711pii
S2213-6711(17)30111-Xjournal_volume
8pub_type
杂志文章abstract::In various vertebrate species, the dorsal aorta (Ao) is the site of specification of adult hematopoietic stem cells (HSCs). It has been observed that the upregulation of essential hematopoietic transcription factors and the formation of specific intra-aortic hematopoietic cell clusters occur predominantly in the ventr...
journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.05.013
更新日期:2016-07-12 00:00:00
abstract::Properties of cancer stem cells involved in drug resistance and relapse have significant effects on clinical outcome. Although tyrosine kinase inhibitors (TKIs) have dramatically improved survival of patients with chronic myeloid leukemia (CML), TKIs have not fully cured CML due to TKI-resistant CML stem cells. Moreov...
journal_title:Stem cell reports
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doi:10.1016/j.stemcr.2018.01.015
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journal_title:Stem cell reports
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doi:10.1016/j.stemcr.2017.06.009
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2017.08.023
更新日期:2017-10-10 00:00:00
abstract::Heterozygous mutations in the glucocerebrosidase gene (GBA) represent the strongest common genetic risk factor for Parkinson's disease (PD), the second most common neurodegenerative disorder. However, the molecular mechanisms underlying this association are still poorly understood. Here, we have analyzed ten independe...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.01.013
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.08.002
更新日期:2016-09-13 00:00:00
abstract::Nerve-derived neural crest cells are essential for regeneration in certain animals, such as newts. Here, we asked whether they play a similar role during mammalian tissue repair, focusing on Sox2-positive neural crest precursors in skin. In adult skin, Sox2 was expressed in nerve-terminal-associated neural crest precu...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2013.04.004
更新日期:2013-06-04 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2015.10.003
更新日期:2015-12-08 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2017.02.006
更新日期:2017-04-11 00:00:00
abstract::Reprogramming of somatic cells produces induced pluripotent stem cells (iPSCs) that are invaluable resources for biomedical research. Here, we extended the previous transcriptome studies by performing RNA-seq on cells defined by a combination of multiple cellular surface markers. We found that transcriptome changes du...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2015.04.009
更新日期:2015-06-09 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2020.01.003
更新日期:2020-02-11 00:00:00
abstract::The International Society for Stem Cell Research 11(th) Annual Meeting was held in Boston in June 2013, bringing together just over 4000 attendees. An emphasis on therapeutic applications in many talks reflected the maturation of the stem cell field from its origins in basic science to one that is beginning to show th...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2013.12.004
更新日期:2013-01-01 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.03.016
更新日期:2018-06-05 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2017.09.016
更新日期:2017-11-14 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2019.07.001
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.06.010
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journal_title:Stem cell reports
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abstract::Intestinal crypts have great capacity for repair and regeneration after intestinal stem cell (ISC) injury. Here, we define the cellular remodeling process resulting from ISC niche interruption by transient Notch pathway inhibition in adult mice. Although ISCs were retained, lineage tracing demonstrated a marked reduct...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2020.05.010
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abstract::Pluripotent stem cells have been proposed as an unlimited source of pancreatic β cells for studying and treating diabetes. However, the long, multi-step differentiation protocols used to generate functional β cells inevitably exhibit considerable variability, particularly when applied to pluripotent cells from diverse...
journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2014.05.010
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
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