Abstract:
:As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation. We applied morpholino-based gene knockdown and cell transplantation strategies to demonstrate that a threshold number of PGCs is required for the stability of ovarian fate. Using histology and transcriptomic analyses, we determined that zebrafish gonads are in a meiotic ovarian stage at 14 days postfertilization and identified signaling pathways supporting meiotic oocyte differentiation and eventual female fate. The development of PGC-depleted gonads appears to be restrained and delayed, suggesting that PGC number may directly regulate the variability and length of gonadal transformation and testicular differentiation in zebrafish. We propose that gonadal transformation may function as a developmental buffering mechanism to ensure the reproductive outcome.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Tzung KW,Goto R,Saju JM,Sreenivasan R,Saito T,Arai K,Yamaha E,Hossain MS,Calvert MEK,Orbán Ldoi
10.1016/j.stemcr.2014.10.011subject
Has Abstractpub_date
2015-01-13 00:00:00pages
61-73issue
1issn
2213-6711pii
S2213-6711(14)00333-6journal_volume
4pub_type
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journal_title:Stem cell reports
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2015.03.002
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journal_title:Stem cell reports
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.11.002
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.05.013
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.01.013
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2015.04.009
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.09.010
更新日期:2018-11-13 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2014.06.002
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.08.009
更新日期:2016-10-11 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章,评审
doi:10.1016/j.stemcr.2019.09.003
更新日期:2019-10-08 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.01.015
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2014.12.012
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journal_title:Stem cell reports
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journal_title:Stem cell reports
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2019.02.009
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2019.12.007
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2020.09.008
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2017.03.019
更新日期:2017-05-09 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2017.09.016
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.08.018
更新日期:2018-10-09 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2019.05.028
更新日期:2019-07-09 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2015.09.012
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