Abstract：
:Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nervous system, provides a platform for the screening of chemical libraries that affect these processes, and offers a potential source of transplantable cells for regenerative approaches to neurological disease. However, defining protocols that permit a large number and high yield of neurons has proved difficult. We present differentiation protocols for the generation of distinct subtypes of neurons in a highly reproducible manner, with minimal experiment-to-experiment variation. These neurons form synapses with neighboring cells, exhibit spontaneous electrical activity, and respond appropriately to depolarization. hPSC-derived neurons exhibit a high degree of maturation and survive in culture for up to 4-5 months, even without astrocyte feeder layers.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Rigamonti A,Repetti GG,Sun C,Price FD,Reny DC,Rapino F,Weisinger K,Benkler C,Peterson QP,Davidow LS,Hansson EM,Rubin LLdoi
10.1016/j.stemcr.2016.05.010subject
Has Abstractpub_date
2016-06-14 00:00:00pages
993-1008issue
6issn
2213-6711pii
S2213-6711(16)30065-0journal_volume
6pub_type
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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