Abstract:
:Both glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells, whereas retinoic acid (RA) induces spermatogonial differentiation. In this study, we investigated the functional differences between FGF2 and GDNF in the germline niche by providing these factors using a drug delivery system in vivo. Although both factors expanded the GFRA1+ subset of undifferentiated spermatogonia, the FGF2-expanded subset expressed RARG, which is indispensable for proper differentiation, 1.9-fold more frequently than the GDNF-expanded subset, demonstrating that FGF2 expands a differentiation-prone subset in the testis. Moreover, FGF2 acted on the germline niche to suppress RA metabolism and GDNF production, suggesting that FGF2 modifies germline niche functions to be more appropriate for spermatogonial differentiation. These results suggest that FGF2 contributes to induction of differentiation rather than maintenance of undifferentiated spermatogonia, indicating reconsideration of the role of FGF2 in the germline niche.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Masaki K,Sakai M,Kuroki S,Jo JI,Hoshina K,Fujimori Y,Oka K,Amano T,Yamanaka T,Tachibana M,Tabata Y,Shiozawa T,Ishizuka O,Hochi S,Takashima Sdoi
10.1016/j.stemcr.2018.03.016subject
Has Abstractpub_date
2018-06-05 00:00:00pages
1782-1792issue
6issn
2213-6711pii
S2213-6711(18)30143-7journal_volume
10pub_type
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