Abstract：
:Pluripotent cells emanate from the inner cell mass (ICM) of the blastocyst and when cultivated under optimal conditions immortalize as embryonic stem cells (ESCs). The fundamental mechanism underlying ESC derivation has, however, remained elusive. Recently, we have devised a highly efficient approach for establishing ESCs, through inhibition of the MEK and TGF-β pathways. This regimen provides a platform for dissecting the molecular mechanism of ESC derivation. Via temporal gene expression analysis, we reveal key genes involved in the ICM to ESC transition. We found that DNA methyltransferases play a pivotal role in efficient ESC generation. We further observed a tight correlation between ESCs and preimplantation epiblast cell-related genes and noticed that fundamental events such as epithelial-to-mesenchymal transition blockage play a key role in launching the ESC self-renewal program. Our study provides a time course transcriptional resource highlighting the dynamics of the gene regulatory network during the ICM to ESC transition.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Totonchi M,Hassani SN,Sharifi-Zarchi A,Tapia N,Adachi K,Arand J,Greber B,Sabour D,Araúzo-Bravo MJ,Walter J,Pakzad M,Gourabi H,Schöler HR,Baharvand Hdoi
10.1016/j.stemcr.2017.08.006subject
Has Abstractpub_date
2017-10-10 00:00:00pages
1275-1290issue
4issn
2213-6711pii
S2213-6711(17)30363-6journal_volume
9pub_type
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