Abstract：
:Microglia, the immune cells of the brain, are crucial to proper development and maintenance of the CNS, and their involvement in numerous neurological disorders is increasingly being recognized. To improve our understanding of human microglial biology, we devised a chemically defined protocol to generate human microglia from pluripotent stem cells. Myeloid progenitors expressing CD14/CX3CR1 were generated within 30 days of differentiation from both embryonic and induced pluripotent stem cells (iPSCs). Further differentiation of the progenitors resulted in ramified microglia with highly motile processes, expressing typical microglial markers. Analyses of gene expression and cytokine release showed close similarities between iPSC-derived (iPSC-MG) and human primary microglia as well as clear distinctions from macrophages. iPSC-MG were able to phagocytose and responded to ADP by producing intracellular Ca2+ transients, whereas macrophages lacked such response. The differentiation protocol was highly reproducible across several pluripotent stem cell lines.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Douvaras P,Sun B,Wang M,Kruglikov I,Lallos G,Zimmer M,Terrenoire C,Zhang B,Gandy S,Schadt E,Freytes DO,Noggle S,Fossati Vdoi
10.1016/j.stemcr.2017.04.023subject
Has Abstractpub_date
2017-06-06 00:00:00pages
1516-1524issue
6issn
2213-6711pii
S2213-6711(17)30177-7journal_volume
8pub_type
杂志文章abstract：:Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nerv...
journal_title：Stem cell reports
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journal_title：Stem cell reports
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