Generation of foxn1/Casper Mutant Zebrafish for Allograft and Xenograft of Normal and Malignant Cells.

abstract：:Thick-filament sarcomere mutations are a common cause of hypertrophic cardiomyopathy (HCM), a disorder of heart muscle thickening associated with sudden cardiac death and heart failure, with unclear mechanisms. We engineered four isogenic induced pluripotent stem cell (iPSC) models of β-myosin heavy chain and myosin-b...

journal_title：Stem cell reports

authors： Cohn R,Thakar K,Lowe A,Ladha FA,Pettinato AM,Romano R,Meredith E,Chen YS,Atamanuk K,Huey BD,Hinson JT

更新日期：2019-01-08 00:00:00

abstract：:We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450-500 nm) is readily observed by fluorescence microscopy and correlates with the expression of pluripotency markers (OCT4,...

journal_title：Stem cell reports

authors： Muthusamy T,Mukherjee O,Menon R,Megha PB,Panicker MM

更新日期：2014-06-12 00:00:00

abstract：:Standardization of mesenchymal stromal cells (MSCs) remains a major obstacle in regenerative medicine. Starting material and culture expansion affect cell preparations and render comparison between studies difficult. In contrast, induced pluripotent stem cells (iPSCs) assimilate toward a ground state and may therefore...

journal_title：Stem cell reports

authors： Frobel J,Hemeda H,Lenz M,Abagnale G,Joussen S,Denecke B,Sarić T,Zenke M,Wagner W

更新日期：2014-09-09 00:00:00

abstract：:Stem cell-derived somatic cells represent an unlimited resource for basic and translational science. Although promising, there are significant hurdles that must be overcome. Our focus is on the generation of the major cell type of the human liver, the hepatocyte. Current protocols produce variable populations of hepat...

journal_title：Stem cell reports

authors： Cameron K,Tan R,Schmidt-Heck W,Campos G,Lyall MJ,Wang Y,Lucendo-Villarin B,Szkolnicka D,Bates N,Kimber SJ,Hengstler JG,Godoy P,Forbes SJ,Hay DC

更新日期：2015-12-08 00:00:00

abstract：:Cardiomyocytes derived from human pluripotent stem cells (hPSCs) are a promising cell source for regenerative medicine, disease modeling, and drug discovery, all of which require enriched cardiomyocytes, ideally ones with mature phenotypes. However, current methods are typically performed in 2D environments that produ...

journal_title：Stem cell reports

authors： Nguyen DC,Hookway TA,Wu Q,Jha R,Preininger MK,Chen X,Easley CA,Spearman P,Deshpande SR,Maher K,Wagner MB,McDevitt TC,Xu C

更新日期：2014-08-12 00:00:00

abstract：:Emerging evidence suggests that Parkinson's disease (PD), besides being an age-associated disorder, might also have a neurodevelopment component. Disruption of mitochondrial homeostasis has been highlighted as a crucial cofactor in its etiology. Here, we show that PD patient-specific human neuroepithelial stem cells (...

journal_title：Stem cell reports

authors： Walter J,Bolognin S,Antony PMA,Nickels SL,Poovathingal SK,Salamanca L,Magni S,Perfeito R,Hoel F,Qing X,Jarazo J,Arias-Fuenzalida J,Ignac T,Monzel AS,Gonzalez-Cano L,Pereira de Almeida L,Skupin A,Tronstad KJ,Schwamborn

更新日期：2019-05-14 00:00:00

abstract：:The International Society for Stem Cell Research 11(th) Annual Meeting was held in Boston in June 2013, bringing together just over 4000 attendees. An emphasis on therapeutic applications in many talks reflected the maturation of the stem cell field from its origins in basic science to one that is beginning to show th...

journal_title：Stem cell reports

authors： Brack AS,Hochedlinger K

更新日期：2013-01-01 00:00:00

abstract：:We identified osteoclast defects in craniometaphyseal dysplasia (CMD) using an easy-to-use protocol for differentiating osteoclasts from human induced pluripotent stem cells (hiPSCs). CMD is a rare genetic bone disorder, characterized by life-long progressive thickening of craniofacial bones and abnormal shape of long...

journal_title：Stem cell reports

authors： Chen IP,Luxmi R,Kanaujiya J,Hao Z,Reichenberger EJ

更新日期：2017-11-14 00:00:00

abstract：:ADP-ribosylation factor-like 4aa (Arl4aa) is a member of the ADP-ribosylation factor family. It is expressed in hematopoietic tissue during embryonic development, but its function was unknown. Zebrafish arl4aa is preferentially expressed in the ventral wall of the dorsal aorta (VDA) at 24 and 36 hpf and in caudal hema...

journal_title：Stem cell reports

authors： Guo Y,Cheng BYL,Wang D,Ma ACH,He BL,Man TK,Cheung MPL,Shi X,Ng NKL,Leung AYH

更新日期：2020-04-14 00:00:00

abstract：:The microenvironment is a critical mediator of stem cell survival, proliferation, migration, and differentiation. The majority of preclinical studies involving transplantation of neural stem cells (NSCs) into the CNS have focused on injured or degenerating microenvironments, leaving a dearth of information as to how N...

journal_title：Stem cell reports

authors： Sontag CJ,Uchida N,Cummings BJ,Anderson AJ

更新日期：2014-04-18 00:00:00

abstract：:Properties of cancer stem cells involved in drug resistance and relapse have significant effects on clinical outcome. Although tyrosine kinase inhibitors (TKIs) have dramatically improved survival of patients with chronic myeloid leukemia (CML), TKIs have not fully cured CML due to TKI-resistant CML stem cells. Moreov...

journal_title：Stem cell reports

authors： Miyauchi M,Koya J,Arai S,Yamazaki S,Honda A,Kataoka K,Yoshimi A,Taoka K,Kumano K,Kurokawa M

更新日期：2018-03-13 00:00:00

abstract：:Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulat...

journal_title：Stem cell reports

authors： Wang S,Wang X,Ma L,Lin X,Zhang D,Li Z,Wu Y,Zheng C,Feng X,Liao S,Feng Y,Chen J,Hu X,Wang M,Han C

更新日期：2016-07-12 00:00:00

abstract：:The in vitro derivation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) is complicated by the existence of multiple overlapping embryonic blood cell programs called primitive, erythromyeloid progenitor (EMP), and definitive. As HSCs are only generated during the definitive stage of hematopoiesis,...

journal_title：Stem cell reports

authors： Clarke RL,Robitaille AM,Moon RT,Keller G

更新日期：2015-08-11 00:00:00

abstract：:Human pluripotent stem cells are a valuable resource for transplantation, yet our ability to profile xenografts is largely limited to low-throughput immunohistochemical analysis by difficulties in readily isolating grafts for transcriptomic and/or proteomic profiling. Here, we present a simple methodology utilizing di...

journal_title：Stem cell reports

authors： Bye CR,Penna V,de Luzy IR,Gantner CW,Hunt CPJ,Thompson LH,Parish CL

更新日期：2019-11-12 00:00:00

abstract：:Direct reprogramming has been widely explored to generate various types of neurons for neurobiological research and translational medicine applications, but there is still no efficient reprogramming method to generate retinal ganglion cell (RGC)-like neurons, which are the sole projection neurons in the retina. Here, ...

journal_title：Stem cell reports

authors： Wang J,He Q,Zhang K,Sun H,Zhang G,Liang H,Guo J,Hao L,Ke J,Chen S

更新日期：2020-11-10 00:00:00

abstract：:Characterization of non-neoplastic and malignant human stem cell populations in their native state can provide new insights into gliomagenesis. Here we developed a purification strategy to directly isolate EGFR+/- populations from human germinal matrix (GM) and adult subventricular zone autopsy tissues, and from de no...

journal_title：Stem cell reports

authors： Tome-Garcia J,Tejero R,Nudelman G,Yong RL,Sebra R,Wang H,Fowkes M,Magid M,Walsh M,Silva-Vargas V,Zaslavsky E,Friedel RH,Doetsch F,Tsankova NM

更新日期：2017-05-09 00:00:00

abstract：:Primary tauopathies are characterized neuropathologically by inclusions containing abnormal forms of the microtubule-associated protein tau (MAPT) and clinically by diverse neuropsychiatric, cognitive, and motor impairments. Autosomal dominant mutations in the MAPT gene cause heterogeneous forms of frontotemporal loba...

journal_title：Stem cell reports

authors： Karch CM,Kao AW,Karydas A,Onanuga K,Martinez R,Argouarch A,Wang C,Huang C,Sohn PD,Bowles KR,Spina S,Silva MC,Marsh JA,Hsu S,Pugh DA,Ghoshal N,Norton J,Huang Y,Lee SE,Seeley WW,Theofilas P,Grinberg LT,Moreno F

更新日期：2019-11-12 00:00:00

abstract：:Nakajo-Nishimura syndrome (NNS) is an immunoproteasome-associated autoinflammatory disorder caused by a mutation of the PSMB8 gene. Although dysfunction of the immunoproteasome causes various cellular stresses attributed to the overproduction of inflammatory cytokines and chemokines in NNS, the underlying mechanisms o...

journal_title：Stem cell reports

authors： Honda-Ozaki F,Terashima M,Niwa A,Saiki N,Kawasaki Y,Ito H,Hotta A,Nagahashi A,Igura K,Asaka I,Li HL,Yanagimachi M,Furukawa F,Kanazawa N,Nakahata T,Saito MK

更新日期：2018-06-05 00:00:00

abstract：:The neural crest (NC) gives rise to a multitude of fetal tissues, and its misregulation is implicated in congenital malformations. Here, we investigated molecular mechanisms pertaining to NC-related symptoms in Bohring-Opitz syndrome (BOS), a developmental disorder linked to mutations in the Polycomb group factor Addi...

journal_title：Stem cell reports

authors： Matheus F,Rusha E,Rehimi R,Molitor L,Pertek A,Modic M,Feederle R,Flatley A,Kremmer E,Geerlof A,Rishko V,Rada-Iglesias A,Drukker M

更新日期：2019-05-14 00:00:00

abstract：:Human embryonic stem cells (hESCs) provide a platform for studying human development and understanding mechanisms underlying diseases. Retinoblastoma-1 (RB1) is a key regulator of cell cycling, of which biallelic inactivation initiates retinoblastoma, the most common congenital intraocular malignancy. We developed a m...

journal_title：Stem cell reports

authors： Avior Y,Lezmi E,Yanuka D,Benvenisty N

更新日期：2017-05-09 00:00:00

abstract：:The small intestine plays an important role in the absorption and metabolism of oral drugs. In the current evaluation system, it is difficult to predict the precise absorption and metabolism of oral drugs. In this study, we generated small intestinal epithelial-like cells from human induced pluripotent stem cells (hiP...

journal_title：Stem cell reports

authors： Negoro R,Takayama K,Kawai K,Harada K,Sakurai F,Hirata K,Mizuguchi H

更新日期：2018-12-11 00:00:00

abstract：:Contractile to synthetic phenotypic switching of smooth muscle cells (SMCs) contributes to stenosis in vascular disease and vascular transplants. To generate more contractile SMCs, we performed a high-throughput differentiation screen using a MYH11-NLuc-tdTomato human embryonic stem cell reporter cell line. We identif...

journal_title：Stem cell reports

authors： Zhang J,McIntosh BE,Wang B,Brown ME,Probasco MD,Webster S,Duffin B,Zhou Y,Guo LW,Burlingham WJ,Kent C,Ferris M,Thomson JA

更新日期：2019-06-11 00:00:00

abstract：:Diminished mitochondrial function is causally related to some heart diseases. Here, we developed a human disease model based on cardiomyocytes from human embryonic stem cells (hESCs), in which an important pathway of mitochondrial gene expression was inactivated. Repression of PGC-1α, which is normally induced during ...

journal_title：Stem cell reports

authors： Birket MJ,Casini S,Kosmidis G,Elliott DA,Gerencser AA,Baartscheer A,Schumacher C,Mastroberardino PG,Elefanty AG,Stanley EG,Mummery CL

更新日期：2013-12-12 00:00:00

abstract：:Reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) entails a mesenchymal to epithelial transition (MET). While attempting to dissect the mechanism of MET during reprogramming, we observed that knockdown (KD) of the epithelial-to-mesenchymal transition (EMT) factor SNAI1 (SNAIL) paradoxically reduce...

journal_title：Stem cell reports

authors： Unternaehrer JJ,Zhao R,Kim K,Cesana M,Powers JT,Ratanasirintrawoot S,Onder T,Shibue T,Weinberg RA,Daley GQ

更新日期：2014-11-11 00:00:00

abstract：:The environment surrounding stem cells has the ability to elicit profound, heritable epigenetic changes orchestrated by multiple epigenetic mechanisms, which can be modulated by the level of specific metabolites. In this review, we highlight the significance of metabolism in regulating stem cell homeostasis, cell stat...

journal_title：Stem cell reports

authors： Harvey A,Caretti G,Moresi V,Renzini A,Adamo S

更新日期：2019-10-08 00:00:00

abstract：:We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use of an optimized CRISPR-Cas9 system together with a double-cut donor increases homology-directed repai...

journal_title：Stem cell reports

authors： Wen W,Cheng X,Fu Y,Meng F,Zhang JP,Zhang L,Li XL,Yang Z,Xu J,Zhang F,Botimer GD,Yuan W,Sun C,Cheng T,Zhang XB

更新日期：2018-06-05 00:00:00

abstract：:A method to maintain and rebuild ureteric bud (UB)-like structures from UB cells in vitro could provide a useful tool for kidney regeneration. We aimed in our present study to establish a serum-free culture system that enables the expansion of UB progenitor cells, i.e., UB tip cells, and reconstruction of UB-like stru...

journal_title：Stem cell reports

authors： Yuri S,Nishikawa M,Yanagawa N,Jo OD,Yanagawa N

更新日期：2017-02-14 00:00:00

abstract：:Differentiation of human embryonic stem cells into pancreatic β cells holds great promise for the treatment of diabetes. Recent advances have led to the production of glucose-responsive insulin-secreting cells in vitro, but resulting cells remain less mature than their adult primary β cell counterparts. The barrier(s)...

journal_title：Stem cell reports

authors： Zhou X,Nair GG,Russ HA,Belair CD,Li ML,Shveygert M,Hebrok M,Blelloch R

更新日期：2020-01-14 00:00:00

abstract：:Genetic changes in human pluripotent stem cells (hPSCs) gained during culture can confound experimental results and potentially jeopardize the outcome of clinical therapies. Particularly common changes in hPSCs are trisomies of chromosomes 1, 12, 17, and 20. Thus, hPSCs should be regularly screened for such aberration...

journal_title：Stem cell reports

authors： Baker D,Hirst AJ,Gokhale PJ,Juarez MA,Williams S,Wheeler M,Bean K,Allison TF,Moore HD,Andrews PW,Barbaric I

更新日期：2016-11-08 00:00:00

abstract：:In various vertebrate species, the dorsal aorta (Ao) is the site of specification of adult hematopoietic stem cells (HSCs). It has been observed that the upregulation of essential hematopoietic transcription factors and the formation of specific intra-aortic hematopoietic cell clusters occur predominantly in the ventr...

journal_title：Stem cell reports

authors： Ivanovs A,Rybtsov S,Anderson RA,Turner ML,Medvinsky A

更新日期：2014-03-27 00:00:00