Abstract：
:Stem cell-derived somatic cells represent an unlimited resource for basic and translational science. Although promising, there are significant hurdles that must be overcome. Our focus is on the generation of the major cell type of the human liver, the hepatocyte. Current protocols produce variable populations of hepatocytes that are the product of using undefined components in the differentiation process. This serves as a significant barrier to scale-up and application. To tackle this issue, we designed a defined differentiation process using recombinant laminin substrates to provide instruction. We demonstrate efficient hepatocyte specification, cell organization, and significant improvements in cell function and phenotype. This is driven in part by the suppression of unfavorable gene regulatory networks that control cell proliferation and migration, pluripotent stem cell self-renewal, and fibroblast and colon specification. We believe that this represents a significant advance, moving stem cell-based hepatocytes closer toward biomedical application.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Cameron K,Tan R,Schmidt-Heck W,Campos G,Lyall MJ,Wang Y,Lucendo-Villarin B,Szkolnicka D,Bates N,Kimber SJ,Hengstler JG,Godoy P,Forbes SJ,Hay DCdoi
10.1016/j.stemcr.2015.10.016subject
Has Abstractpub_date
2015-12-08 00:00:00pages
1250-1262issue
6issn
2213-6711pii
S2213-6711(15)00313-6journal_volume
5pub_type
杂志文章abstract：:The environment surrounding stem cells has the ability to elicit profound, heritable epigenetic changes orchestrated by multiple epigenetic mechanisms, which can be modulated by the level of specific metabolites. In this review, we highlight the significance of metabolism in regulating stem cell homeostasis, cell stat...
journal_title：Stem cell reports
pub_type： 杂志文章,评审
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journal_title：Stem cell reports
pub_type： 信件
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abstract：:Predicting developmental potency and risk of posttransplantation tumor formation by human pluripotent stem cells (hPSCs) and their derivatives largely rely on classical histological analysis of teratomas. Here, we investigated whether an assay based on microRNAs (miRNA) in blood plasma is able to detect potentially ma...
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abstract：:Pluripotent cells emanate from the inner cell mass (ICM) of the blastocyst and when cultivated under optimal conditions immortalize as embryonic stem cells (ESCs). The fundamental mechanism underlying ESC derivation has, however, remained elusive. Recently, we have devised a highly efficient approach for establishing ...
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pub_type： 杂志文章
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abstract：:Nerve-derived neural crest cells are essential for regeneration in certain animals, such as newts. Here, we asked whether they play a similar role during mammalian tissue repair, focusing on Sox2-positive neural crest precursors in skin. In adult skin, Sox2 was expressed in nerve-terminal-associated neural crest precu...
journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2015.09.012
更新日期：2015-11-10 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章,评审
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2017.05.023
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.03.021
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2016.12.026
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journal_title：Stem cell reports
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
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abstract：:Primordial germ cells (PGCs) are lineage-restricted unipotent cells that can dedifferentiate into pluripotent embryonic germ cells (EGCs). Here we performed whole-transcriptome analysis during the conversion of PGCs into EGCs, a process by which cells acquire pluripotency. To examine the molecular mechanism underlying...
journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.06.010
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2013.06.001
更新日期：2013-07-11 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章,评审
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journal_title：Stem cell reports
pub_type： 杂志文章
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abstract：:Direct reprogramming of adult somatic cells into alternative cell types has been shown for several lineages. We previously showed that GATA4, MEF2C, and TBX5 (GMT) directly reprogrammed nonmyocyte mouse heart cells into induced cardiomyocyte-like cells (iCMs) in vitro and in vivo. However, GMT alone appears insufficie...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2013.07.005
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