LNGFR(+)THY-1(+)VCAM-1(hi+) cells reveal functionally distinct subpopulations in mesenchymal stem cells.

abstract：:Genetic changes in human pluripotent stem cells (hPSCs) gained during culture can confound experimental results and potentially jeopardize the outcome of clinical therapies. Particularly common changes in hPSCs are trisomies of chromosomes 1, 12, 17, and 20. Thus, hPSCs should be regularly screened for such aberration...

journal_title：Stem cell reports

authors： Baker D,Hirst AJ,Gokhale PJ,Juarez MA,Williams S,Wheeler M,Bean K,Allison TF,Moore HD,Andrews PW,Barbaric I

更新日期：2016-11-08 00:00:00

abstract：:Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput ima...

journal_title：Stem cell reports

authors： Larribère L,Galach M,Novak D,Arévalo K,Volz HC,Stark HJ,Boukamp P,Boutros M,Utikal J

更新日期：2017-10-10 00:00:00

abstract：:Insertion of reporter cassettes into the Lgr5 locus has enabled the characterization of mouse intestinal stem cells (ISCs). However, low cell surface abundance of LGR5 protein and lack of high-affinity anti-LGR5 antibodies represent a roadblock to efficiently isolate human colonic stem cells (hCoSCs). We set out to id...

journal_title：Stem cell reports

authors： Jung P,Sommer C,Barriga FM,Buczacki SJ,Hernando-Momblona X,Sevillano M,Duran-Frigola M,Aloy P,Selbach M,Winton DJ,Batlle E

更新日期：2015-12-08 00:00:00

abstract：:The hematopoietic marrow microenvironment is composed of multiple cell types embedded in an extracellular matrix (ECM). We have explored marrow ECM using mass spectrometry and found dermatopontin (DPT), a small non-collagenous ECM protein, to be present. We found that DPT cooperates with other ECM proteins to promote ...

journal_title：Stem cell reports

authors： Kramer AC,Blake AL,Taisto ME,Lehrke MJ,Webber BR,Lund TC

更新日期：2017-09-12 00:00:00

abstract：:The rigorous characterization of distinct induced pluripotent stem cells (iPSC) derived from multiple reprogramming technologies, somatic sources, and donors is required to understand potential sources of variability and downstream potential. To achieve this goal, the Progenitor Cell Biology Consortium performed compr...

journal_title：Stem cell reports

authors： Salomonis N,Dexheimer PJ,Omberg L,Schroll R,Bush S,Huo J,Schriml L,Ho Sui S,Keddache M,Mayhew C,Shanmukhappa SK,Wells J,Daily K,Hubler S,Wang Y,Zambidis E,Margolin A,Hide W,Hatzopoulos AK,Malik P,Cancelas JA,Aro

更新日期：2016-07-12 00:00:00

abstract：:Stem cell-derived somatic cells represent an unlimited resource for basic and translational science. Although promising, there are significant hurdles that must be overcome. Our focus is on the generation of the major cell type of the human liver, the hepatocyte. Current protocols produce variable populations of hepat...

journal_title：Stem cell reports

authors： Cameron K,Tan R,Schmidt-Heck W,Campos G,Lyall MJ,Wang Y,Lucendo-Villarin B,Szkolnicka D,Bates N,Kimber SJ,Hengstler JG,Godoy P,Forbes SJ,Hay DC

更新日期：2015-12-08 00:00:00

abstract：:Direct cardiac reprogramming holds great potential for regenerative medicine. However, it remains inefficient, and induced cardiomyocytes (iCMs) generated in vitro are less mature than those in vivo, suggesting that undefined extrinsic factors may regulate cardiac reprogramming. Previous in vitro studies mainly used h...

journal_title：Stem cell reports

authors： Kurotsu S,Sadahiro T,Fujita R,Tani H,Yamakawa H,Tamura F,Isomi M,Kojima H,Yamada Y,Abe Y,Murakata Y,Akiyama T,Muraoka N,Harada I,Suzuki T,Fukuda K,Ieda M

更新日期：2020-09-08 00:00:00

abstract：:In human-to-mouse xenograft models, reconstitution of human hematopoiesis is usually B-lymphoid dominant. Here we show that the introduction of homozygous Kit(Wv) mutations into C57BL/6.Rag2(null)Il2rg(null) mice with NOD-Sirpa (BRGS) strongly promoted human multi-lineage reconstitution. After xenotransplantation of h...

journal_title：Stem cell reports

authors： Yurino A,Takenaka K,Yamauchi T,Nunomura T,Uehara Y,Jinnouchi F,Miyawaki K,Kikushige Y,Kato K,Miyamoto T,Iwasaki H,Kunisaki Y,Akashi K

更新日期：2016-09-13 00:00:00

abstract：:Hepatic steatosis is a highly prevalent liver disease, yet research is hampered by the lack of tractable cellular and animal models. Steatosis also occurs in cats, where it can cause severe hepatic failure. Previous studies demonstrate the potential of liver organoids for modeling genetic diseases. To examine the poss...

journal_title：Stem cell reports

authors： Kruitwagen HS,Oosterhoff LA,Vernooij IGWH,Schrall IM,van Wolferen ME,Bannink F,Roesch C,van Uden L,Molenaar MR,Helms JB,Grinwis GCM,Verstegen MMA,van der Laan LJW,Huch M,Geijsen N,Vries RG,Clevers H,Rothuizen J,Schota

更新日期：2017-04-11 00:00:00

abstract：:Pelizaeus-Merzbacher disease (PMD) is a fatal X-linked disorder caused by loss of myelinating oligodendrocytes and consequent hypomyelination. The underlying cellular and molecular dysfunctions are not fully defined, but therapeutic enhancement of oligodendrocyte survival could restore functional myelination in patien...

journal_title：Stem cell reports

authors： Elitt MS,Shick HE,Madhavan M,Allan KC,Clayton BLL,Weng C,Miller TE,Factor DC,Barbar L,Nawash BS,Nevin ZS,Lager AM,Li Y,Jin F,Adams DJ,Tesar PJ

更新日期：2018-09-11 00:00:00

abstract：:Thyroid hormone (TH) transporters are required for the transmembrane passage of TH in target cells. In humans, inactivating mutations in the TH transporter MCT8 cause the Allan-Herndon-Dudley syndrome, characterized by severe neuromuscular symptoms and an abnormal TH serum profile, which is fully replicated in Mct8 kn...

journal_title：Stem cell reports

authors： Mayerl S,Schmidt M,Doycheva D,Darras VM,Hüttner SS,Boelen A,Visser TJ,Kaether C,Heuer H,von Maltzahn J

更新日期：2018-06-05 00:00:00

abstract：:Reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) entails a mesenchymal to epithelial transition (MET). While attempting to dissect the mechanism of MET during reprogramming, we observed that knockdown (KD) of the epithelial-to-mesenchymal transition (EMT) factor SNAI1 (SNAIL) paradoxically reduce...

journal_title：Stem cell reports

authors： Unternaehrer JJ,Zhao R,Kim K,Cesana M,Powers JT,Ratanasirintrawoot S,Onder T,Shibue T,Weinberg RA,Daley GQ

更新日期：2014-11-11 00:00:00

abstract：:Hematopoietic stem cells (HSCs) exist in a dormant state and progressively lose regenerative potency as they undergo successive divisions. Why this functional decline occurs and how this information is encoded is unclear. To better understand how this information is stored, we performed RNA sequencing on HSC populatio...

journal_title：Stem cell reports

authors： Bernitz JM,Rapp K,Daniel MG,Shcherbinin D,Yuan Y,Gomes A,Waghray A,Brosh R,Lachmann A,Ma'ayan A,Papatsenko D,Moore KA

更新日期：2020-04-14 00:00:00

abstract：:Primitive erythropoiesis follows a stereotypic developmental program of mesoderm ventralization and internalization, hemangioblast formation and migration, and erythroid lineage specification. Induction of erythropoiesis is inefficient in either ES/iPS cells in vitro or nonhemangioblast cell populations in vivo. Using...

journal_title：Stem cell reports

authors： Weng W,Sheng G

更新日期：2014-03-06 00:00:00

abstract：:We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450-500 nm) is readily observed by fluorescence microscopy and correlates with the expression of pluripotency markers (OCT4,...

journal_title：Stem cell reports

authors： Muthusamy T,Mukherjee O,Menon R,Megha PB,Panicker MM

更新日期：2014-06-12 00:00:00

abstract：:Mesenchymal stem cells (MSCs) can donate mitochondria and rescue anthracycline-induced cardiomyocyte (CM) damage, although the underlying mechanisms remain elusive. We determined that the superior efficiency of mitochondrial transfer by human induced-pluripotent-stem-cell-derived MSCs (iPSC-MSCs) compared with bone ma...

journal_title：Stem cell reports

authors： Zhang Y,Yu Z,Jiang D,Liang X,Liao S,Zhang Z,Yue W,Li X,Chiu SM,Chai YH,Liang Y,Chow Y,Han S,Xu A,Tse HF,Lian Q

更新日期：2016-10-11 00:00:00

abstract：:Thick-filament sarcomere mutations are a common cause of hypertrophic cardiomyopathy (HCM), a disorder of heart muscle thickening associated with sudden cardiac death and heart failure, with unclear mechanisms. We engineered four isogenic induced pluripotent stem cell (iPSC) models of β-myosin heavy chain and myosin-b...

journal_title：Stem cell reports

authors： Cohn R,Thakar K,Lowe A,Ladha FA,Pettinato AM,Romano R,Meredith E,Chen YS,Atamanuk K,Huey BD,Hinson JT

更新日期：2019-01-08 00:00:00

abstract：:The ability to form teratomas in vivo containing multiple somatic cell types is regarded as functional evidence of pluripotency for human pluripotent stem cells (hPSCs). Since the Teratoma assay is animal dependent, laborious, and only qualitative, the PluriTest and the hPSC ScoreCard assay have been developed as in v...

journal_title：Stem cell reports

authors： Bouma MJ,van Iterson M,Janssen B,Mummery CL,Salvatori DCF,Freund C

更新日期：2017-05-09 00:00:00

abstract：:We earlier showed that outside-in integrin signaling through POSTN-ITGAV interaction plays an important role in regulating adult hematopoietic stem cell (HSC) quiescence. Here, we show that Itgav deletion results in increased frequency of phenotypic HSCs in fetal liver (FL) due to faster proliferation. Systemic deleti...

journal_title：Stem cell reports

authors： Biswas A,Roy IM,Babu PC,Manesia J,Schouteden S,Vijayakurup V,Anto RJ,Huelsken J,Lacy-Hulbert A,Verfaillie CM,Khurana S

更新日期：2020-08-11 00:00:00

abstract：:Robust production of terminally differentiated cells from self-renewing resident stem cells is essential to maintain proper tissue architecture and physiological functions, especially in high-turnover tissues. However, the transcriptional networks that precisely regulate cell transition and differentiation are poorly ...

journal_title：Stem cell reports

authors： Meng FW,Rojas Villa SE,Biteau B

更新日期：2020-02-11 00:00:00

abstract：:Microglia, the immune cells of the brain, are crucial to proper development and maintenance of the CNS, and their involvement in numerous neurological disorders is increasingly being recognized. To improve our understanding of human microglial biology, we devised a chemically defined protocol to generate human microgl...

journal_title：Stem cell reports

authors： Douvaras P,Sun B,Wang M,Kruglikov I,Lallos G,Zimmer M,Terrenoire C,Zhang B,Gandy S,Schadt E,Freytes DO,Noggle S,Fossati V

更新日期：2017-06-06 00:00:00

abstract：:Human pluripotent stem cells are a valuable resource for transplantation, yet our ability to profile xenografts is largely limited to low-throughput immunohistochemical analysis by difficulties in readily isolating grafts for transcriptomic and/or proteomic profiling. Here, we present a simple methodology utilizing di...

journal_title：Stem cell reports

authors： Bye CR,Penna V,de Luzy IR,Gantner CW,Hunt CPJ,Thompson LH,Parish CL

更新日期：2019-11-12 00:00:00

abstract：:Diminished mitochondrial function is causally related to some heart diseases. Here, we developed a human disease model based on cardiomyocytes from human embryonic stem cells (hESCs), in which an important pathway of mitochondrial gene expression was inactivated. Repression of PGC-1α, which is normally induced during ...

journal_title：Stem cell reports

authors： Birket MJ,Casini S,Kosmidis G,Elliott DA,Gerencser AA,Baartscheer A,Schumacher C,Mastroberardino PG,Elefanty AG,Stanley EG,Mummery CL

更新日期：2013-12-12 00:00:00

abstract：:Activation of Wnt/β-catenin signaling can induce both self-renewal and differentiation in naive pluripotent embryonic stem cells (ESCs). To gain insights into the mechanism by which Wnt/β-catenin regulates ESC fate, we screened and characterized its downstream targets. Here, we show that the self-renewal-promoting eff...

journal_title：Stem cell reports

authors： Qiu D,Ye S,Ruiz B,Zhou X,Liu D,Zhang Q,Ying QL

更新日期：2015-09-08 00:00:00

abstract：:The in vitro derivation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) is complicated by the existence of multiple overlapping embryonic blood cell programs called primitive, erythromyeloid progenitor (EMP), and definitive. As HSCs are only generated during the definitive stage of hematopoiesis,...

journal_title：Stem cell reports

authors： Clarke RL,Robitaille AM,Moon RT,Keller G

更新日期：2015-08-11 00:00:00

abstract：:The Polycomb group gene BMI1 is essential for efficient muscle regeneration in a mouse model of Duchenne muscular dystrophy, and its enhanced expression in adult skeletal muscle satellite cells ameliorates the muscle strength in this model. Here, we show that the impact of mild BMI1 overexpression observed in mouse mo...

journal_title：Stem cell reports

authors： Dibenedetto S,Niklison-Chirou M,Cabrera CP,Ellis M,Robson LG,Knopp P,Tedesco FS,Ragazzi M,Di Foggia V,Barnes MR,Radunovic A,Marino S

更新日期：2017-08-08 00:00:00

abstract：:Tight regulation of the balance between self-renewal and differentiation of neural stem cells is crucial to assure proper neural development. In this context, Notch signaling is a well-known promoter of stemness. In contrast, the bifunctional brain-enriched microRNA miR-9/9(∗) has been implicated in promoting neuronal...

journal_title：Stem cell reports

authors： Roese-Koerner B,Stappert L,Berger T,Braun NC,Veltel M,Jungverdorben J,Evert BO,Peitz M,Borghese L,Brüstle O

更新日期：2016-08-09 00:00:00

abstract：:Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 106 hiPSCs per layer yiel...

journal_title：Stem cell reports

authors： Tohyama S,Fujita J,Fujita C,Yamaguchi M,Kanaami S,Ohno R,Sakamoto K,Kodama M,Kurokawa J,Kanazawa H,Seki T,Kishino Y,Okada M,Nakajima K,Tanosaki S,Someya S,Hirano A,Kawaguchi S,Kobayashi E,Fukuda K

更新日期：2017-11-14 00:00:00

abstract：:Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate new neurons capable of func...

journal_title：Stem cell reports

authors： Kannangara TS,Carter A,Xue Y,Dhaliwal JS,Béïque JC,Lagace DC

更新日期：2018-12-11 00:00:00

abstract：:The first hematopoietic stem and progenitor cells are generated during development from hemogenic endothelium (HE) through trans-differentiation. The molecular mechanisms underlying this endothelial-to-hematopoietic transition (EHT) remain poorly understood. Here, we explored the role of the epigenetic regulators HDAC...

journal_title：Stem cell reports

authors： Thambyrajah R,Fadlullah MZH,Proffitt M,Patel R,Cowley SM,Kouskoff V,Lacaud G

更新日期：2018-04-10 00:00:00