Abstract:
:Primitive erythropoiesis follows a stereotypic developmental program of mesoderm ventralization and internalization, hemangioblast formation and migration, and erythroid lineage specification. Induction of erythropoiesis is inefficient in either ES/iPS cells in vitro or nonhemangioblast cell populations in vivo. Using the chick model, we report that epiblast cells can be directly and efficiently differentiated into the erythroid lineage by expressing five hematopoietic transcription regulators (SCL+LMO2+GATA2+LDB1+E2A) and inhibiting the FGF pathway. We show that these five genes are expressed with temporal specificity during normal erythropoiesis. Initiation of SCL and LMO2 expression requires FGF activity, whereas erythroid differentiation is enhanced by FGF inhibition. The lag between hematopoiesis and erythropoiesis is attributed to sequential coregulator expression and hemangioblast migration. Globin gene transcription can be ectopically and prematurely induced by manipulating the availability of these factors and the FGF pathway activity. We propose that similar approaches can be taken for efficient erythroid differentiation in vitro.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Weng W,Sheng Gdoi
10.1016/j.stemcr.2014.01.019subject
Has Abstractpub_date
2014-03-06 00:00:00pages
262-70issue
3issn
2213-6711pii
S2213-6711(14)00053-8journal_volume
2pub_type
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journal_title:Stem cell reports
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pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
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pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.09.010
更新日期:2018-11-13 00:00:00
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/j.stemcr.2019.05.028
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.12.016
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pub_type: 杂志文章
doi:10.1016/j.stemcr.2019.08.008
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journal_title:Stem cell reports
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journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2014.02.004
更新日期:2014-03-27 00:00:00
abstract::BACE1 is validated as Alzheimer's β-secretase and a therapeutic target for Alzheimer's disease. In examining BACE1-null mice, we discovered that BACE1 deficiency develops abnormal clusters of immature neurons, forming doublecortin-positive neuroblasts, in the developing dentate gyrus, mainly in the subpial zone (SPZ)....
journal_title:Stem cell reports
pub_type: 杂志文章
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.11.015
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journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.07.016
更新日期:2016-09-13 00:00:00
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journal_title:Stem cell reports
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journal_title:Stem cell reports
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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