当前位置： SCI文献检索 > Stem Cell Reports期刊下所有文献 > Transcriptional Profiling of Xenogeneic Transplants: Examining Human Pluripotent Stem Cell-Derived Grafts in the Rodent Brain.

Transcriptional Profiling of Xenogeneic Transplants: Examining Human Pluripotent Stem Cell-Derived Grafts in the Rodent Brain.

Abstract：

:Human pluripotent stem cells are a valuable resource for transplantation, yet our ability to profile xenografts is largely limited to low-throughput immunohistochemical analysis by difficulties in readily isolating grafts for transcriptomic and/or proteomic profiling. Here, we present a simple methodology utilizing differences in the RNA sequence between species to discriminate xenograft from host gene expression (using qPCR or RNA sequencing [RNA-seq]). To demonstrate the approach, we assessed grafts of undifferentiated human stem cells and neural progenitors in the rodent brain. Xenograft-specific qPCR provided sensitive detection of proliferative cells, and identified germ layer markers and appropriate neural maturation genes across the graft types. Xenograft-specific RNA-seq enabled profiling of the complete transcriptome and an unbiased characterization of graft composition. Such xenograft-specific profiling will be crucial for pre-clinical characterization of grafts and batch-testing of therapeutic cell preparations to ensure safety and functional predictability prior to translation.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Bye CR,Penna V,de Luzy IR,Gantner CW,Hunt CPJ,Thompson LH,Parish CL

doi

10.1016/j.stemcr.2019.10.001

subject

Has Abstract

pub_date

2019-11-12 00:00:00

pages

877-890

issue

5

issn

2213-6711

pii

S2213-6711(19)30362-5

journal_volume

13

pub_type

杂志文章

相关文献

Stem Cell Reports文献大全
  • Inhibition of Gata4 and Tbx5 by Nicotine-Mediated DNA Methylation in Myocardial Differentiation.

    abstract：:Maternal nicotine exposure causes alteration of gene expression and cardiovascular programming. The discovery of nicotine-medicated regulation in cardiogenesis is of major importance for the study of cardiac defects. The present study investigated the effect of nicotine on cardiac gene expression and epigenetic regula...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.12.016

    authors： Jiang XY,Feng YL,Ye LT,Li XH,Feng J,Zhang MZ,Shelat HS,Wassler M,Li Y,Geng YJ,Yu XY

    更新日期：2017-02-14 00:00:00

  • Reciprocal Regulation between Bifunctional miR-9/9(∗) and its Transcriptional Modulator Notch in Human Neural Stem Cell Self-Renewal and Differentiation.

    abstract：:Tight regulation of the balance between self-renewal and differentiation of neural stem cells is crucial to assure proper neural development. In this context, Notch signaling is a well-known promoter of stemness. In contrast, the bifunctional brain-enriched microRNA miR-9/9(∗) has been implicated in promoting neuronal...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.06.008

    authors： Roese-Koerner B,Stappert L,Berger T,Braun NC,Veltel M,Jungverdorben J,Evert BO,Peitz M,Borghese L,Brüstle O

    更新日期：2016-08-09 00:00:00

  • FGF2 Has Distinct Molecular Functions from GDNF in the Mouse Germline Niche.

    abstract：:Both glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells, whereas retinoic acid (RA) induces spermatogonial differentiation. In this study, we investigated the functional differences between FGF2 and GDNF in the germl...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.03.016

    authors： Masaki K,Sakai M,Kuroki S,Jo JI,Hoshina K,Fujimori Y,Oka K,Amano T,Yamanaka T,Tachibana M,Tabata Y,Shiozawa T,Ishizuka O,Hochi S,Takashima S

    更新日期：2018-06-05 00:00:00

  • Stable X chromosome reactivation in female human induced pluripotent stem cells.

    abstract：:In placental mammals, balanced expression of X-linked genes is accomplished by X chromosome inactivation (XCI) in female cells. In humans, random XCI is initiated early during embryonic development. To investigate whether reprogramming of female human fibroblasts into induced pluripotent stem cells (iPSCs) leads to re...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.12.012

    authors： Barakat TS,Ghazvini M,de Hoon B,Li T,Eussen B,Douben H,van der Linden R,van der Stap N,Boter M,Laven JS,Galjaard RJ,Grootegoed JA,de Klein A,Gribnau J

    更新日期：2015-02-10 00:00:00

  • Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke.

    abstract：:Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate new neurons capable of func...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.10.011

    authors： Kannangara TS,Carter A,Xue Y,Dhaliwal JS,Béïque JC,Lagace DC

    更新日期：2018-12-11 00:00:00

  • Microglia-Secreted Factors Enhance Dopaminergic Differentiation of Tissue- and iPSC-Derived Human Neural Stem Cells.

    abstract：:Microglia have recently been established as key regulators of brain development. However, their role in neuronal subtype specification remains largely unknown. Using three different co-culture setups, we show that microglia-secreted factors enhance dopaminergic differentiation of somatic and induced pluripotent stem c...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.12.011

    authors： Schmidt SI,Bogetofte H,Ritter L,Agergaard JB,Hammerich D,Kabiljagic AA,Wlodarczyk A,Lopez SG,Sørensen MD,Jørgensen ML,Okarmus J,Serrano AM,Kristensen BW,Freude K,Owens T,Meyer M

    更新日期：2021-01-06 00:00:00

  • High-Level Precise Knockin of iPSCs by Simultaneous Reprogramming and Genome Editing of Human Peripheral Blood Mononuclear Cells.

    abstract：:We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use of an optimized CRISPR-Cas9 system together with a double-cut donor increases homology-directed repai...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.04.013

    authors： Wen W,Cheng X,Fu Y,Meng F,Zhang JP,Zhang L,Li XL,Yang Z,Xu J,Zhang F,Botimer GD,Yuan W,Sun C,Cheng T,Zhang XB

    更新日期：2018-06-05 00:00:00

  • Interplay between Metabolites and the Epigenome in Regulating Embryonic and Adult Stem Cell Potency and Maintenance.

    abstract：:The environment surrounding stem cells has the ability to elicit profound, heritable epigenetic changes orchestrated by multiple epigenetic mechanisms, which can be modulated by the level of specific metabolites. In this review, we highlight the significance of metabolism in regulating stem cell homeostasis, cell stat...

    journal_title：Stem cell reports

    pub_type： 杂志文章,评审

    doi：10.1016/j.stemcr.2019.09.003

    authors： Harvey A,Caretti G,Moresi V,Renzini A,Adamo S

    更新日期：2019-10-08 00:00:00

  • Chemical Screening Identifies Enhancers of Mutant Oligodendrocyte Survival and Unmasks a Distinct Pathological Phase in Pelizaeus-Merzbacher Disease.

    abstract：:Pelizaeus-Merzbacher disease (PMD) is a fatal X-linked disorder caused by loss of myelinating oligodendrocytes and consequent hypomyelination. The underlying cellular and molecular dysfunctions are not fully defined, but therapeutic enhancement of oligodendrocyte survival could restore functional myelination in patien...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.07.015

    authors： Elitt MS,Shick HE,Madhavan M,Allan KC,Clayton BLL,Weng C,Miller TE,Factor DC,Barbar L,Nawash BS,Nevin ZS,Lager AM,Li Y,Jin F,Adams DJ,Tesar PJ

    更新日期：2018-09-11 00:00:00

  • Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes.

    abstract：:Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 106 hiPSCs per layer yiel...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.08.025

    authors： Tohyama S,Fujita J,Fujita C,Yamaguchi M,Kanaami S,Ohno R,Sakamoto K,Kodama M,Kurokawa J,Kanazawa H,Seki T,Kishino Y,Okada M,Nakajima K,Tanosaki S,Someya S,Hirano A,Kawaguchi S,Kobayashi E,Fukuda K

    更新日期：2017-11-14 00:00:00

  • Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes.

    abstract：:Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulat...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.05.013

    authors： Wang S,Wang X,Ma L,Lin X,Zhang D,Li Z,Wu Y,Zheng C,Feng X,Liao S,Feng Y,Chen J,Hu X,Wang M,Han C

    更新日期：2016-07-12 00:00:00

  • Induced Pluripotent Stem Cells Reprogrammed with Three Inhibitors Show Accelerated Differentiation Potentials with High Levels of 2-Cell Stage Marker Expression.

    abstract：:Although pluripotent stem cells can generate various types of differentiated cells, it is unclear why lineage-committed stem/progenitor cells derived from pluripotent stem cells are decelerated and why the differentiation-resistant propensity of embryonic stem cell (ESC)/induced pluripotent stem cell (iPSC)-derived ce...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.12.018

    authors： Nishihara K,Shiga T,Nakamura E,Akiyama T,Sasaki T,Suzuki S,Ko MSH,Tada N,Okano H,Akamatsu W

    更新日期：2019-02-12 00:00:00

  • Characterizing Direct-to-Consumer Stem Cell Businesses in the Southwest United States.

    abstract：:There are currently hundreds of businesses across the United States offering direct-to-consumer stem cell treatments that have not been through regulatory approval by the Food and Drug Administration (FDA). Here, we provide a detailed characterization of nearly 170 stem cell businesses operating in the Southwest Unite...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.07.001

    authors： Frow EK,Brafman DA,Muldoon A,Krum L,Williams P,Becker B,Nelson JP,Pritchett A

    更新日期：2019-08-13 00:00:00

  • Maternal inflammation contributes to brain overgrowth and autism-associated behaviors through altered redox signaling in stem and progenitor cells.

    abstract：:A period of mild brain overgrowth with an unknown etiology has been identified as one of the most common phenotypes in autism. Here, we test the hypothesis that maternal inflammation during critical periods of embryonic development can cause brain overgrowth and autism-associated behaviors as a result of altered neura...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.09.004

    authors： Le Belle JE,Sperry J,Ngo A,Ghochani Y,Laks DR,López-Aranda M,Silva AJ,Kornblum HI

    更新日期：2014-11-11 00:00:00

  • FAM122A Inhibits Erythroid Differentiation through GATA1.

    abstract：:FAM122A is a highly conserved housekeeping gene, but its physiological and pathophysiological roles remain greatly elusive. Based on the fact that FAM122A is highly expressed in human CD71+ early erythroid cells, herein we report that FAM122A is downregulated during erythroid differentiation, while its overexpression ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.07.010

    authors： Chen J,Zhou Q,Liu MH,Yang YS,Wang YQ,Huang Y,Chen GQ

    更新日期：2020-09-08 00:00:00

  • Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models.

    abstract：:Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.10.003

    authors： Sugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi M

    更新日期：2017-11-14 00:00:00

  • BACE1 Deficiency Causes Abnormal Neuronal Clustering in the Dentate Gyrus.

    abstract：:BACE1 is validated as Alzheimer's β-secretase and a therapeutic target for Alzheimer's disease. In examining BACE1-null mice, we discovered that BACE1 deficiency develops abnormal clusters of immature neurons, forming doublecortin-positive neuroblasts, in the developing dentate gyrus, mainly in the subpial zone (SPZ)....

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.05.030

    authors： Hou H,Fan Q,He W,Suh H,Hu X,Yan R

    更新日期：2017-07-11 00:00:00

  • Phenotypic Screening Identifies Modulators of Amyloid Precursor Protein Processing in Human Stem Cell Models of Alzheimer's Disease.

    abstract：:Human stem cell models have the potential to provide platforms for phenotypic screens to identify candidate treatments and cellular pathways involved in the pathogenesis of neurodegenerative disorders. Amyloid precursor protein (APP) processing and the accumulation of APP-derived amyloid β (Aβ) peptides are key proces...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.02.006

    authors： Brownjohn PW,Smith J,Portelius E,Serneels L,Kvartsberg H,De Strooper B,Blennow K,Zetterberg H,Livesey FJ

    更新日期：2017-04-11 00:00:00

  • LIN28B Impairs the Transition of hESC-Derived β Cells from the Juvenile to Adult State.

    abstract：:Differentiation of human embryonic stem cells into pancreatic β cells holds great promise for the treatment of diabetes. Recent advances have led to the production of glucose-responsive insulin-secreting cells in vitro, but resulting cells remain less mature than their adult primary β cell counterparts. The barrier(s)...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.11.009

    authors： Zhou X,Nair GG,Russ HA,Belair CD,Li ML,Shveygert M,Hebrok M,Blelloch R

    更新日期：2020-01-14 00:00:00

  • Rapid Crypt Cell Remodeling Regenerates the Intestinal Stem Cell Niche after Notch Inhibition.

    abstract：:Intestinal crypts have great capacity for repair and regeneration after intestinal stem cell (ISC) injury. Here, we define the cellular remodeling process resulting from ISC niche interruption by transient Notch pathway inhibition in adult mice. Although ISCs were retained, lineage tracing demonstrated a marked reduct...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.05.010

    authors： Bohin N,Keeley TM,Carulli AJ,Walker EM,Carlson EA,Gao J,Aifantis I,Siebel CW,Rajala MW,Myers MG Jr,Jones JC,Brindley CD,Dempsey PJ,Samuelson LC

    更新日期：2020-07-14 00:00:00

  • In Vitro Differentiated Human Stem Cell-Derived Neurons Reproduce Synaptic Synchronicity Arising during Neurodevelopment.

    abstract：:Neurons differentiated from induced pluripotent stem cells (iPSCs) typically show regular spiking and synaptic activity but lack more complex network activity critical for brain development, such as periodic depolarizations including simultaneous involvement of glutamatergic and GABAergic neurotransmission. We generat...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.05.015

    authors： Rosa F,Dhingra A,Uysal B,Mendis GDC,Loeffler H,Elsen G,Mueller S,Schwarz N,Castillo-Lizardo M,Cuddy C,Becker F,Heutink P,Reid CA,Petrou S,Lerche H,Maljevic S

    更新日期：2020-07-14 00:00:00

  • LNGFR(+)THY-1(+)VCAM-1(hi+) cells reveal functionally distinct subpopulations in mesenchymal stem cells.

    abstract：:Human mesenchymal stem cells (hMSCs), which conventionally are isolated based on their adherence to plastic, are heterogeneous and have poor growth and differentiation, limiting our ability to investigate their intrinsic characteristics. We report an improved prospective clonal isolation technique and reveal that the ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.06.001

    authors： Mabuchi Y,Morikawa S,Harada S,Niibe K,Suzuki S,Renault-Mihara F,Houlihan DD,Akazawa C,Okano H,Matsuzaki Y

    更新日期：2013-07-11 00:00:00

  • Soft Matrix Promotes Cardiac Reprogramming via Inhibition of YAP/TAZ and Suppression of Fibroblast Signatures.

    abstract：:Direct cardiac reprogramming holds great potential for regenerative medicine. However, it remains inefficient, and induced cardiomyocytes (iCMs) generated in vitro are less mature than those in vivo, suggesting that undefined extrinsic factors may regulate cardiac reprogramming. Previous in vitro studies mainly used h...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.07.022

    authors： Kurotsu S,Sadahiro T,Fujita R,Tani H,Yamakawa H,Tamura F,Isomi M,Kojima H,Yamada Y,Abe Y,Murakata Y,Akiyama T,Muraoka N,Harada I,Suzuki T,Fukuda K,Ieda M

    更新日期：2020-09-08 00:00:00

  • PGC-1α and reactive oxygen species regulate human embryonic stem cell-derived cardiomyocyte function.

    abstract：:Diminished mitochondrial function is causally related to some heart diseases. Here, we developed a human disease model based on cardiomyocytes from human embryonic stem cells (hESCs), in which an important pathway of mitochondrial gene expression was inactivated. Repression of PGC-1α, which is normally induced during ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.11.008

    authors： Birket MJ,Casini S,Kosmidis G,Elliott DA,Gerencser AA,Baartscheer A,Schumacher C,Mastroberardino PG,Elefanty AG,Stanley EG,Mummery CL

    更新日期：2013-12-12 00:00:00

  • 20th Anniversary of Isolation of Human Embryonic Stem Cells: A Personal Perspective.

    abstract：:Following Jamie Thomson's lecture on primate embryonic stem cells (ESCs) at a meeting I had organized in March 1997, in Israel, to celebrate receipt of the Wolf Prize in Agriculture to my colleague and friend Neal First, frozen human embryos donated for research in Israel were shipped to Wisconsin. The five hESC lines...

    journal_title：Stem cell reports

    pub_type： 杂志文章,评审

    doi：10.1016/j.stemcr.2018.04.011

    authors： Itskovitz-Eldor J

    更新日期：2018-05-08 00:00:00

  • TeratoScore: Assessing the Differentiation Potential of Human Pluripotent Stem Cells by Quantitative Expression Analysis of Teratomas.

    abstract：:Teratoma formation is the gold standard assay for testing the capacity of human pluripotent stem cells to differentiate into all embryonic germ layers. Although widely used, little effort has been made to transform this qualitative assay into a quantitative one. Using gene expression data from a wide variety of cells,...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.05.006

    authors： Avior Y,Biancotti JC,Benvenisty N

    更新日期：2015-06-09 00:00:00

  • The epithelial-mesenchymal transition factor SNAIL paradoxically enhances reprogramming.

    abstract：:Reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) entails a mesenchymal to epithelial transition (MET). While attempting to dissect the mechanism of MET during reprogramming, we observed that knockdown (KD) of the epithelial-to-mesenchymal transition (EMT) factor SNAI1 (SNAIL) paradoxically reduce...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.09.008

    authors： Unternaehrer JJ,Zhao R,Kim K,Cesana M,Powers JT,Ratanasirintrawoot S,Onder T,Shibue T,Weinberg RA,Daley GQ

    更新日期：2014-11-11 00:00:00

  • Developmental IL-6 Exposure Favors Production of PDGF-Responsive Multipotential Progenitors at the Expense of Neural Stem Cells and Other Progenitors.

    abstract：:Interleukin-6 (IL-6) is increased in maternal serum and amniotic fluid of children subsequently diagnosed with autism spectrum disorders. However, it is not clear how increased IL-6 alters brain development. Here, we show that IL-6 increases the prevalence of a specific platelet-derived growth factor (PDGF)-responsive...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.03.019

    authors： Kumari E,Velloso FJ,Nasuhidehnavi A,Somasundaram A,Savanur VH,Buono KD,Levison SW

    更新日期：2020-05-12 00:00:00

  • ISSCR 2013: back to Bean Town.

    abstract：:The International Society for Stem Cell Research 11(th) Annual Meeting was held in Boston in June 2013, bringing together just over 4000 attendees. An emphasis on therapeutic applications in many talks reflected the maturation of the stem cell field from its origins in basic science to one that is beginning to show th...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.12.004

    authors： Brack AS,Hochedlinger K

    更新日期：2013-01-01 00:00:00

  • Induced Developmental Arrest of Early Hematopoietic Progenitors Leads to the Generation of Leukocyte Stem Cells.

    abstract：:Self-renewal potential and multipotency are hallmarks of a stem cell. It is generally accepted that acquisition of such stemness requires rejuvenation of somatic cells through reprogramming of their genetic and epigenetic status.We show here that a simple block of cell differentiation is sufficient to induce and maint...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.09.012

    authors： Ikawa T,Masuda K,Huijskens MJAJ,Satoh R,Kakugawa K,Agata Y,Miyai T,Germeraad WTV,Katsura Y,Kawamoto H

    更新日期：2015-11-10 00:00:00