ISSCR 2013: back to Bean Town.

abstract：:BACE1 is validated as Alzheimer's β-secretase and a therapeutic target for Alzheimer's disease. In examining BACE1-null mice, we discovered that BACE1 deficiency develops abnormal clusters of immature neurons, forming doublecortin-positive neuroblasts, in the developing dentate gyrus, mainly in the subpial zone (SPZ)....

journal_title：Stem cell reports

authors： Hou H,Fan Q,He W,Suh H,Hu X,Yan R

更新日期：2017-07-11 00:00:00

abstract：:In addition to their promise in regenerative medicine, pluripotent stem cells have proved to be faithful models of many human diseases. In particular, patient-specific stem cell-derived cardiomyocytes recapitulate key features of several life-threatening cardiac arrhythmia syndromes. For both modeling and regenerative...

journal_title：Stem cell reports

authors： Leyton-Mange JS,Mills RW,Macri VS,Jang MY,Butte FN,Ellinor PT,Milan DJ

更新日期：2014-02-06 00:00:00

abstract：:The in vitro derivation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) is complicated by the existence of multiple overlapping embryonic blood cell programs called primitive, erythromyeloid progenitor (EMP), and definitive. As HSCs are only generated during the definitive stage of hematopoiesis,...

journal_title：Stem cell reports

authors： Clarke RL,Robitaille AM,Moon RT,Keller G

更新日期：2015-08-11 00:00:00

abstract：:Nakajo-Nishimura syndrome (NNS) is an immunoproteasome-associated autoinflammatory disorder caused by a mutation of the PSMB8 gene. Although dysfunction of the immunoproteasome causes various cellular stresses attributed to the overproduction of inflammatory cytokines and chemokines in NNS, the underlying mechanisms o...

journal_title：Stem cell reports

authors： Honda-Ozaki F,Terashima M,Niwa A,Saiki N,Kawasaki Y,Ito H,Hotta A,Nagahashi A,Igura K,Asaka I,Li HL,Yanagimachi M,Furukawa F,Kanazawa N,Nakahata T,Saito MK

更新日期：2018-06-05 00:00:00

abstract：:Predicting developmental potency and risk of posttransplantation tumor formation by human pluripotent stem cells (hPSCs) and their derivatives largely rely on classical histological analysis of teratomas. Here, we investigated whether an assay based on microRNAs (miRNA) in blood plasma is able to detect potentially ma...

journal_title：Stem cell reports

authors： Salvatori DCF,Dorssers LCJ,Gillis AJM,Perretta G,van Agthoven T,Gomes Fernandes M,Stoop H,Prins JB,Oosterhuis JW,Mummery C,Looijenga LHJ

更新日期：2018-12-11 00:00:00

abstract：:The balance of self-renewal and differentiation in long-term repopulating hematopoietic stem cells (LT-HSC) must be strictly controlled to maintain blood homeostasis and to prevent leukemogenesis. Hematopoietic cytokines can induce differentiation in LT-HSCs; however, the molecular mechanism orchestrating this delicat...

journal_title：Stem cell reports

authors： Thalheimer FB,Wingert S,De Giacomo P,Haetscher N,Rehage M,Brill B,Theis FJ,Hennighausen L,Schroeder T,Rieger MA

更新日期：2014-06-19 00:00:00

abstract：:The environment surrounding stem cells has the ability to elicit profound, heritable epigenetic changes orchestrated by multiple epigenetic mechanisms, which can be modulated by the level of specific metabolites. In this review, we highlight the significance of metabolism in regulating stem cell homeostasis, cell stat...

journal_title：Stem cell reports

authors： Harvey A,Caretti G,Moresi V,Renzini A,Adamo S

更新日期：2019-10-08 00:00:00

abstract：:Heterozygous de novo mutations in GATA6 are the most frequent cause of pancreatic agenesis in humans. In mice, however, a similar phenotype requires the biallelic loss of Gata6 and its paralog Gata4. To elaborate the human-specific requirements for GATA6, we chose to model GATA6 loss in vitro by combining both gene-ed...

journal_title：Stem cell reports

authors： Chia CY,Madrigal P,Denil SLIJ,Martinez I,Garcia-Bernardo J,El-Khairi R,Chhatriwala M,Shepherd MH,Hattersley AT,Dunn NR,Vallier L

更新日期：2019-01-08 00:00:00

abstract：:Nerve-derived neural crest cells are essential for regeneration in certain animals, such as newts. Here, we asked whether they play a similar role during mammalian tissue repair, focusing on Sox2-positive neural crest precursors in skin. In adult skin, Sox2 was expressed in nerve-terminal-associated neural crest precu...

journal_title：Stem cell reports

authors： Johnston AP,Naska S,Jones K,Jinno H,Kaplan DR,Miller FD

更新日期：2013-06-04 00:00:00

abstract：:Developing effective therapeutics for complex diseases such as late-onset, sporadic Alzheimer's disease (SAD) is difficult due to genetic and environmental heterogeneity in the human population and the limitations of existing animal models. Here, we used hiPSC-derived neurons to test a compound that stabilizes the ret...

journal_title：Stem cell reports

authors： Young JE,Fong LK,Frankowski H,Petsko GA,Small SA,Goldstein LSB

更新日期：2018-03-13 00:00:00

abstract：:Thyroid hormone (TH) transporters are required for the transmembrane passage of TH in target cells. In humans, inactivating mutations in the TH transporter MCT8 cause the Allan-Herndon-Dudley syndrome, characterized by severe neuromuscular symptoms and an abnormal TH serum profile, which is fully replicated in Mct8 kn...

journal_title：Stem cell reports

authors： Mayerl S,Schmidt M,Doycheva D,Darras VM,Hüttner SS,Boelen A,Visser TJ,Kaether C,Heuer H,von Maltzahn J

更新日期：2018-06-05 00:00:00

abstract：:Direct reprogramming has been widely explored to generate various types of neurons for neurobiological research and translational medicine applications, but there is still no efficient reprogramming method to generate retinal ganglion cell (RGC)-like neurons, which are the sole projection neurons in the retina. Here, ...

journal_title：Stem cell reports

authors： Wang J,He Q,Zhang K,Sun H,Zhang G,Liang H,Guo J,Hao L,Ke J,Chen S

更新日期：2020-11-10 00:00:00

abstract：:Deletion of caudal/cdx genes alters hox gene expression and causes defects in posterior tissues and hematopoiesis. Yet, the defects in hox gene expression only partially explain these phenotypes. To gain deeper insight into Cdx4 function, we performed chromatin immunoprecipitation sequencing (ChIP-seq) combined with g...

journal_title：Stem cell reports

authors： Paik EJ,Mahony S,White RM,Price EN,Dibiase A,Dorjsuren B,Mosimann C,Davidson AJ,Gifford D,Zon LI

更新日期：2013-11-07 00:00:00

abstract：:The first hematopoietic stem and progenitor cells are generated during development from hemogenic endothelium (HE) through trans-differentiation. The molecular mechanisms underlying this endothelial-to-hematopoietic transition (EHT) remain poorly understood. Here, we explored the role of the epigenetic regulators HDAC...

journal_title：Stem cell reports

authors： Thambyrajah R,Fadlullah MZH,Proffitt M,Patel R,Cowley SM,Kouskoff V,Lacaud G

更新日期：2018-04-10 00:00:00

abstract：:Human vision relies heavily upon cone photoreceptors, and their loss results in permanent visual impairment. Transplantation of healthy photoreceptors can restore visual function in models of inherited blindness, a process previously understood to arise by donor cell integration within the host retina. However, we and...

journal_title：Stem cell reports

authors： Waldron PV,Di Marco F,Kruczek K,Ribeiro J,Graca AB,Hippert C,Aghaizu ND,Kalargyrou AA,Barber AC,Grimaldi G,Duran Y,Blackford SJI,Kloc M,Goh D,Zabala Aldunate E,Sampson RD,Bainbridge JWB,Smith AJ,Gonzalez-Cordero A,S

更新日期：2018-02-13 00:00:00

abstract：:Direct reprogramming of adult somatic cells into alternative cell types has been shown for several lineages. We previously showed that GATA4, MEF2C, and TBX5 (GMT) directly reprogrammed nonmyocyte mouse heart cells into induced cardiomyocyte-like cells (iCMs) in vitro and in vivo. However, GMT alone appears insufficie...

journal_title：Stem cell reports

authors： Fu JD,Stone NR,Liu L,Spencer CI,Qian L,Hayashi Y,Delgado-Olguin P,Ding S,Bruneau BG,Srivastava D

更新日期：2013-08-22 00:00:00

abstract：:The microenvironment is a critical mediator of stem cell survival, proliferation, migration, and differentiation. The majority of preclinical studies involving transplantation of neural stem cells (NSCs) into the CNS have focused on injured or degenerating microenvironments, leaving a dearth of information as to how N...

journal_title：Stem cell reports

authors： Sontag CJ,Uchida N,Cummings BJ,Anderson AJ

更新日期：2014-04-18 00:00:00

abstract：:Microglia have recently been established as key regulators of brain development. However, their role in neuronal subtype specification remains largely unknown. Using three different co-culture setups, we show that microglia-secreted factors enhance dopaminergic differentiation of somatic and induced pluripotent stem c...

journal_title：Stem cell reports

authors： Schmidt SI,Bogetofte H,Ritter L,Agergaard JB,Hammerich D,Kabiljagic AA,Wlodarczyk A,Lopez SG,Sørensen MD,Jørgensen ML,Okarmus J,Serrano AM,Kristensen BW,Freude K,Owens T,Meyer M

更新日期：2021-01-06 00:00:00

abstract：:Differentiation of human embryonic stem cells into pancreatic β cells holds great promise for the treatment of diabetes. Recent advances have led to the production of glucose-responsive insulin-secreting cells in vitro, but resulting cells remain less mature than their adult primary β cell counterparts. The barrier(s)...

journal_title：Stem cell reports

authors： Zhou X,Nair GG,Russ HA,Belair CD,Li ML,Shveygert M,Hebrok M,Blelloch R

更新日期：2020-01-14 00:00:00

abstract：:The chromatin of naive embryonic stem cells (ESCs) has a largely open configuration, as evident by the lack of condensed heterochromatin and the hypomethylation of DNA. Several molecular mechanisms promoting this constellation were previously identified. Here we present evidence for an important epigenetic function of...

journal_title：Stem cell reports

authors： Rahjouei A,Pirouz M,Di Virgilio M,Kamin D,Kessel M

更新日期：2017-04-11 00:00:00

abstract：:B-lymphocyte-induced nuclear maturation protein 1 (BLIMP1) was previously reported to define a sebaceous gland (SG) progenitor population in the epidermis. However, the recent identification of multiple stem cell populations in the hair follicle junctional zone has led us to re-evaluate its function. We show, in agree...

journal_title：Stem cell reports

authors： Kretzschmar K,Cottle DL,Donati G,Chiang MF,Quist SR,Gollnick HP,Natsuga K,Lin KI,Watt FM

更新日期：2014-10-14 00:00:00

abstract：:Non-human primates (NHPs) can serve as a human-like model to study cell therapy using induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). However, whether the efficacy of NHP and human iPSC-CMs is mechanistically similar remains unknown. To examine this, RNU rats received intramyocardial injection of 1 × ...

journal_title：Stem cell reports

authors： Zhao X,Chen H,Xiao D,Yang H,Itzhaki I,Qin X,Chour T,Aguirre A,Lehmann K,Kim Y,Shukla P,Holmström A,Zhang JZ,Zhuge Y,Ndoye BC,Zhao M,Neofytou E,Zimmermann WH,Jain M,Wu JC

更新日期：2018-02-13 00:00:00

abstract：:As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation. We applied morpholin...

journal_title：Stem cell reports

authors： Tzung KW,Goto R,Saju JM,Sreenivasan R,Saito T,Arai K,Yamaha E,Hossain MS,Calvert MEK,Orbán L

更新日期：2015-01-13 00:00:00

abstract：:Dynamic spatiotemporal modification of chromatin around DNA damage is vital for efficient DNA repair. Normal stem cells exhibit an attenuated DNA damage response (DDR), inefficient DNA repair, and high radiosensitivity. The impact of unique chromatin characteristics of stem cells in DDR regulation is not yet recognize...

journal_title：Stem cell reports

authors： Meyer B,Fabbrizi MR,Raj S,Zobel CL,Hallahan DE,Sharma GG

更新日期：2016-12-13 00:00:00

abstract：:In human-to-mouse xenograft models, reconstitution of human hematopoiesis is usually B-lymphoid dominant. Here we show that the introduction of homozygous Kit(Wv) mutations into C57BL/6.Rag2(null)Il2rg(null) mice with NOD-Sirpa (BRGS) strongly promoted human multi-lineage reconstitution. After xenotransplantation of h...

journal_title：Stem cell reports

authors： Yurino A,Takenaka K,Yamauchi T,Nunomura T,Uehara Y,Jinnouchi F,Miyawaki K,Kikushige Y,Kato K,Miyamoto T,Iwasaki H,Kunisaki Y,Akashi K

更新日期：2016-09-13 00:00:00

abstract：:Teratoma formation is the gold standard assay for testing the capacity of human pluripotent stem cells to differentiate into all embryonic germ layers. Although widely used, little effort has been made to transform this qualitative assay into a quantitative one. Using gene expression data from a wide variety of cells,...

journal_title：Stem cell reports

authors： Avior Y,Biancotti JC,Benvenisty N

更新日期：2015-06-09 00:00:00

abstract：:Mitochondria are critical to neurogenesis, but the mechanisms of mitochondria in neurogenesis have not been well explored. We fully characterized mitochondrial alterations and function in relation to the development of human induced pluripotent stem cell (hiPSC)-derived dopaminergic (DA) neurons. Following directed di...

journal_title：Stem cell reports

authors： Fang D,Qing Y,Yan S,Chen D,Yan SS

更新日期：2016-10-11 00:00:00

abstract：:Fanconi anemia (FA) causes bone marrow failure early during childhood, and recent studies indicate that a hematopoietic defect could begin in utero. We performed a unique kinetics study of hematopoiesis in Fancg-/- mouse embryos, between the early embryonic day 11.5 (E11.5) to E12.5 developmental window (when the high...

journal_title：Stem cell reports

authors： Domenech C,Maillard L,Rousseau A,Guidez F,Petit L,Pla M,Clay D,Guimiot F,Sanfilippo S,Jacques S,de la Grange P,Robil N,Soulier J,Souyri M

更新日期：2018-11-13 00:00:00

abstract：:Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 106 hiPSCs per layer yiel...

journal_title：Stem cell reports

authors： Tohyama S,Fujita J,Fujita C,Yamaguchi M,Kanaami S,Ohno R,Sakamoto K,Kodama M,Kurokawa J,Kanazawa H,Seki T,Kishino Y,Okada M,Nakajima K,Tanosaki S,Someya S,Hirano A,Kawaguchi S,Kobayashi E,Fukuda K

更新日期：2017-11-14 00:00:00

abstract：:Neural progenitor cells (NPCs) transplanted into sites of spinal cord injury (SCI) extend large numbers of axons into the caudal host spinal cord. We determined the precise locations of neurons in the graft that extend axons into the caudal host spinal cord using AAV9-Cre-initiated retrograde tracing into floxed-TdTom...

journal_title：Stem cell reports

authors： Lu P,Gomes-Leal W,Anil S,Dobkins G,Huie JR,Ferguson AR,Graham L,Tuszynski M

更新日期：2019-07-09 00:00:00