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Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner.

Abstract：

:Human vision relies heavily upon cone photoreceptors, and their loss results in permanent visual impairment. Transplantation of healthy photoreceptors can restore visual function in models of inherited blindness, a process previously understood to arise by donor cell integration within the host retina. However, we and others recently demonstrated that donor rod photoreceptors engage in material transfer with host photoreceptors, leading to the host cells acquiring proteins otherwise expressed only by donor cells. We sought to determine whether stem cell- and donor-derived cones undergo integration and/or material transfer. We find that material transfer accounts for a significant proportion of rescued cells following cone transplantation into non-degenerative hosts. Strikingly, however, substantial numbers of cones integrated into the Nrl-/- and Prph2rd2/rd2, but not Nrl-/-;RPE65R91W/R91W, murine models of retinal degeneration. This confirms the occurrence of photoreceptor integration in certain models of retinal degeneration and demonstrates the importance of the host environment in determining transplantation outcome.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Waldron PV,Di Marco F,Kruczek K,Ribeiro J,Graca AB,Hippert C,Aghaizu ND,Kalargyrou AA,Barber AC,Grimaldi G,Duran Y,Blackford SJI,Kloc M,Goh D,Zabala Aldunate E,Sampson RD,Bainbridge JWB,Smith AJ,Gonzalez-Cordero A,S

doi

10.1016/j.stemcr.2017.12.008

subject

Has Abstract

pub_date

2018-02-13 00:00:00

pages

406-421

issue

2

issn

2213-6711

pii

S2213-6711(17)30556-8

journal_volume

10

pub_type

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