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Prospective Isolation and Comparison of Human Germinal Matrix and Glioblastoma EGFR+ Populations with Stem Cell Properties.

Abstract：

:Characterization of non-neoplastic and malignant human stem cell populations in their native state can provide new insights into gliomagenesis. Here we developed a purification strategy to directly isolate EGFR+/- populations from human germinal matrix (GM) and adult subventricular zone autopsy tissues, and from de novo glioblastoma (GBM) resections, enriching for cells capable of binding EGF ligand (LBEGFR+), and uniquely compared their functional and molecular properties. LBEGFR+ populations in both GM and GBM encompassed all sphere-forming cells and displayed proliferative stem cell properties in vitro. In xenografts, LBEGFR+ GBM cells showed robust tumor initiation and progression to high-grade, infiltrative gliomas. Whole-transcriptome sequencing analysis confirmed enrichment of proliferative pathways in both developing and neoplastic freshly isolated EGFR+ populations, and identified both unique and shared sets of genes. The ability to prospectively isolate stem cell populations using native ligand-binding capacity opens new doors onto understanding both normal human development and tumor cell biology.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Tome-Garcia J,Tejero R,Nudelman G,Yong RL,Sebra R,Wang H,Fowkes M,Magid M,Walsh M,Silva-Vargas V,Zaslavsky E,Friedel RH,Doetsch F,Tsankova NM

doi

10.1016/j.stemcr.2017.03.019

subject

Has Abstract

pub_date

2017-05-09 00:00:00

pages

1421-1429

issue

5

issn

2213-6711

pii

S2213-6711(17)30125-X

journal_volume

8

pub_type

杂志文章

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