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Microscale generation of cardiospheres promotes robust enrichment of cardiomyocytes derived from human pluripotent stem cells.

Abstract：

:Cardiomyocytes derived from human pluripotent stem cells (hPSCs) are a promising cell source for regenerative medicine, disease modeling, and drug discovery, all of which require enriched cardiomyocytes, ideally ones with mature phenotypes. However, current methods are typically performed in 2D environments that produce immature cardiomyocytes within heterogeneous populations. Here, we generated 3D aggregates of cardiomyocytes (cardiospheres) from 2D differentiation cultures of hPSCs using microscale technology and rotary orbital suspension culture. Nearly 100% of the cardiospheres showed spontaneous contractility and synchronous intracellular calcium transients. Strikingly, from starting heterogeneous populations containing ∼10%-40% cardiomyocytes, the cell population within the generated cardiospheres featured ∼80%-100% cardiomyocytes, corresponding to an enrichment factor of up to 7-fold. Furthermore, cardiomyocytes from cardiospheres exhibited enhanced structural maturation in comparison with those from a parallel 2D culture. Thus, generation of cardiospheres represents a simple and robust method for enrichment of cardiomyocytes in microtissues that have the potential use in regenerative medicine as well as other applications.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Nguyen DC,Hookway TA,Wu Q,Jha R,Preininger MK,Chen X,Easley CA,Spearman P,Deshpande SR,Maher K,Wagner MB,McDevitt TC,Xu C

doi

10.1016/j.stemcr.2014.06.002

subject

Has Abstract

pub_date

2014-08-12 00:00:00

pages

260-8

issue

2

issn

2213-6711

pii

S2213-6711(14)00188-X

journal_volume

3

pub_type

杂志文章

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