Enhanced Energetic State and Protection from Oxidative Stress in Human Myoblasts Overexpressing BMI1.

abstract：:Fanconi anemia (FA) causes bone marrow failure early during childhood, and recent studies indicate that a hematopoietic defect could begin in utero. We performed a unique kinetics study of hematopoiesis in Fancg-/- mouse embryos, between the early embryonic day 11.5 (E11.5) to E12.5 developmental window (when the high...

journal_title：Stem cell reports

authors： Domenech C,Maillard L,Rousseau A,Guidez F,Petit L,Pla M,Clay D,Guimiot F,Sanfilippo S,Jacques S,de la Grange P,Robil N,Soulier J,Souyri M

更新日期：2018-11-13 00:00:00

abstract：:Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput ima...

journal_title：Stem cell reports

authors： Larribère L,Galach M,Novak D,Arévalo K,Volz HC,Stark HJ,Boukamp P,Boutros M,Utikal J

更新日期：2017-10-10 00:00:00

abstract：:Developing effective therapeutics for complex diseases such as late-onset, sporadic Alzheimer's disease (SAD) is difficult due to genetic and environmental heterogeneity in the human population and the limitations of existing animal models. Here, we used hiPSC-derived neurons to test a compound that stabilizes the ret...

journal_title：Stem cell reports

authors： Young JE,Fong LK,Frankowski H,Petsko GA,Small SA,Goldstein LSB

更新日期：2018-03-13 00:00:00

abstract：:We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use of an optimized CRISPR-Cas9 system together with a double-cut donor increases homology-directed repai...

journal_title：Stem cell reports

authors： Wen W,Cheng X,Fu Y,Meng F,Zhang JP,Zhang L,Li XL,Yang Z,Xu J,Zhang F,Botimer GD,Yuan W,Sun C,Cheng T,Zhang XB

更新日期：2018-06-05 00:00:00

abstract：:Genetic changes in human pluripotent stem cells (hPSCs) gained during culture can confound experimental results and potentially jeopardize the outcome of clinical therapies. Particularly common changes in hPSCs are trisomies of chromosomes 1, 12, 17, and 20. Thus, hPSCs should be regularly screened for such aberration...

journal_title：Stem cell reports

authors： Baker D,Hirst AJ,Gokhale PJ,Juarez MA,Williams S,Wheeler M,Bean K,Allison TF,Moore HD,Andrews PW,Barbaric I

更新日期：2016-11-08 00:00:00

abstract：:Induced pluripotent stem cells were created from a pancreas agenesis patient with a mutation in GATA6. Using genome-editing technology, additional stem cell lines with mutations in both GATA6 alleles were generated and demonstrated a severe block in definitive endoderm induction, which could be rescued by re-expressio...

journal_title：Stem cell reports

authors： Tiyaboonchai A,Cardenas-Diaz FL,Ying L,Maguire JA,Sim X,Jobaliya C,Gagne AL,Kishore S,Stanescu DE,Hughes N,De Leon DD,French DL,Gadue P

更新日期：2017-03-14 00:00:00

abstract：:Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during...

journal_title：Stem cell reports

authors： Pasqualini FS,Sheehy SP,Agarwal A,Aratyn-Schaus Y,Parker KK

更新日期：2015-03-10 00:00:00

abstract：:Direct reprogramming has been widely explored to generate various types of neurons for neurobiological research and translational medicine applications, but there is still no efficient reprogramming method to generate retinal ganglion cell (RGC)-like neurons, which are the sole projection neurons in the retina. Here, ...

journal_title：Stem cell reports

authors： Wang J,He Q,Zhang K,Sun H,Zhang G,Liang H,Guo J,Hao L,Ke J,Chen S

更新日期：2020-11-10 00:00:00

abstract：:The recent development of targeted murine reporter alleles as proxies for intestinal stem cell activity has led to significant advances in our understanding of somatic stem cell hierarchies and dynamics. Analysis of these reporters has led to a model in which an indispensable reserve stem cell at the top of the hierar...

journal_title：Stem cell reports

authors： Li N,Yousefi M,Nakauka-Ddamba A,Jain R,Tobias J,Epstein JA,Jensen ST,Lengner CJ

更新日期：2014-11-11 00:00:00

abstract：:We earlier showed that outside-in integrin signaling through POSTN-ITGAV interaction plays an important role in regulating adult hematopoietic stem cell (HSC) quiescence. Here, we show that Itgav deletion results in increased frequency of phenotypic HSCs in fetal liver (FL) due to faster proliferation. Systemic deleti...

journal_title：Stem cell reports

authors： Biswas A,Roy IM,Babu PC,Manesia J,Schouteden S,Vijayakurup V,Anto RJ,Huelsken J,Lacy-Hulbert A,Verfaillie CM,Khurana S

更新日期：2020-08-11 00:00:00

abstract：:Primordial germ cells (PGCs) are fate restricted to differentiate into gametes in vivo. However, when removed from their embryonic niche, PGCs undergo reversion to pluripotent embryonic germ cells (EGCs) in vitro. One of the major differences between EGCs and embryonic stem cells (ESCs) is variable methylation at impr...

journal_title：Stem cell reports

authors： Oliveros-Etter M,Li Z,Nee K,Hosohama L,Hargan-Calvopina J,Lee SA,Joti P,Yu J,Clark AT

更新日期：2015-09-08 00:00:00

abstract：:In this article, Scott describes how a new counseling profession could support clinical sites and patients enrolling in stem cell clinical trials. A possible model is proposed, along with a curriculum that would provide counselors with the tools to address challenges facing the clinical stem cell field. Finally, a can...

journal_title：Stem cell reports

authors： Scott CT

更新日期：2015-01-13 00:00:00

abstract：:Pro-fibrotic microenvironments of scars and tumors characterized by increased stiffness stimulate mesenchymal stromal cells (MSCs) to express α-smooth muscle actin (α-SMA). We investigated whether incorporation of α-SMA into contractile stress fibers regulates human MSC fate. Sorted α-SMA-positive MSCs exhibited high ...

journal_title：Stem cell reports

authors： Talele NP,Fradette J,Davies JE,Kapus A,Hinz B

更新日期：2015-06-09 00:00:00

abstract：:The differentiation of pluripotent stem cells to hepatocytes is well established, yet current methods suffer from several drawbacks. These include a lack of definition and reproducibility, which in part stems from continued reliance on recombinant growth factors. This has remained a stumbling block for the translation...

journal_title：Stem cell reports

authors： Siller R,Greenhough S,Naumovska E,Sullivan GJ

更新日期：2015-05-12 00:00:00

abstract：:Spermatogonial stem cells (SSCs) serve as a resource for producing genetically modified animals. However, genetic manipulation of SSCs has met with limited success. Here, we show efficient gene transfer into SSCs via a lentivirus (FV-LV) using a fusion protein (F), a Sendai virus (SV) envelope protein involved in viri...

journal_title：Stem cell reports

authors： Shinohara T,Kanatsu-Shinohara M

更新日期：2020-03-10 00:00:00

abstract：:Microglia have recently been established as key regulators of brain development. However, their role in neuronal subtype specification remains largely unknown. Using three different co-culture setups, we show that microglia-secreted factors enhance dopaminergic differentiation of somatic and induced pluripotent stem c...

journal_title：Stem cell reports

authors： Schmidt SI,Bogetofte H,Ritter L,Agergaard JB,Hammerich D,Kabiljagic AA,Wlodarczyk A,Lopez SG,Sørensen MD,Jørgensen ML,Okarmus J,Serrano AM,Kristensen BW,Freude K,Owens T,Meyer M

更新日期：2021-01-06 00:00:00

abstract：:Primary tauopathies are characterized neuropathologically by inclusions containing abnormal forms of the microtubule-associated protein tau (MAPT) and clinically by diverse neuropsychiatric, cognitive, and motor impairments. Autosomal dominant mutations in the MAPT gene cause heterogeneous forms of frontotemporal loba...

journal_title：Stem cell reports

authors： Karch CM,Kao AW,Karydas A,Onanuga K,Martinez R,Argouarch A,Wang C,Huang C,Sohn PD,Bowles KR,Spina S,Silva MC,Marsh JA,Hsu S,Pugh DA,Ghoshal N,Norton J,Huang Y,Lee SE,Seeley WW,Theofilas P,Grinberg LT,Moreno F

更新日期：2019-11-12 00:00:00

abstract：:The balance between factors leading to proliferation and differentiation of cortical neural precursors (CNPs) determines the correct cortical development. In this work, we show that GDNF and its receptor GFRα1 are expressed in the neocortex during the period of cortical neurogenesis. We show that the GDNF/GFRα1 comple...

journal_title：Stem cell reports

authors： Bonafina A,Fontanet PA,Paratcha G,Ledda F

更新日期：2018-03-13 00:00:00

abstract：:Pig embryonic stem cells (pESCs) have been considered an important candidate for preclinical research on human therapies. However, the lack of understanding of pig pluripotent networks has hampered establishment of authentic pESCs. Here, we report that FGF2, ACTVIN, and WNT signaling are essential to sustain pig pluri...

journal_title：Stem cell reports

authors： Choi KH,Lee DK,Kim SW,Woo SH,Kim DY,Lee CK

更新日期：2019-07-09 00:00:00

abstract：:Cardiomyocytes derived from human pluripotent stem cells (hPSCs) are a promising cell source for regenerative medicine, disease modeling, and drug discovery, all of which require enriched cardiomyocytes, ideally ones with mature phenotypes. However, current methods are typically performed in 2D environments that produ...

journal_title：Stem cell reports

authors： Nguyen DC,Hookway TA,Wu Q,Jha R,Preininger MK,Chen X,Easley CA,Spearman P,Deshpande SR,Maher K,Wagner MB,McDevitt TC,Xu C

更新日期：2014-08-12 00:00:00

abstract：:Human mesenchymal stem cells (hMSCs), which conventionally are isolated based on their adherence to plastic, are heterogeneous and have poor growth and differentiation, limiting our ability to investigate their intrinsic characteristics. We report an improved prospective clonal isolation technique and reveal that the ...

journal_title：Stem cell reports

authors： Mabuchi Y,Morikawa S,Harada S,Niibe K,Suzuki S,Renault-Mihara F,Houlihan DD,Akazawa C,Okano H,Matsuzaki Y

更新日期：2013-07-11 00:00:00

abstract：:Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate new neurons capable of func...

journal_title：Stem cell reports

authors： Kannangara TS,Carter A,Xue Y,Dhaliwal JS,Béïque JC,Lagace DC

更新日期：2018-12-11 00:00:00

abstract：:Mammalian haploid embryonic stem cells (haESCs) hold great promise for functional genetic studies and forward screening. However, all established haploid cells are prone to spontaneous diploidization during long-term culture, rendering application challenging. Here, we report a genome-wide loss-of-function screening t...

journal_title：Stem cell reports

authors： Zhang G,Li X,Sun Y,Wang X,Liu G,Huang Y

更新日期：2021-01-12 00:00:00

abstract：:When comparing hepatic phenotypes between iPSC-derived hepatocyte-like cells from different liver disease patients, cell heterogeneity can confound interpretation. We proposed that homogeneous cell populations could be generated by fluorescence-activated cell sorting (FACS). Using cell-surface capture proteomics, we i...

journal_title：Stem cell reports

authors： Mallanna SK,Cayo MA,Twaroski K,Gundry RL,Duncan SA

更新日期：2016-09-13 00:00:00

abstract：:The hematopoietic marrow microenvironment is composed of multiple cell types embedded in an extracellular matrix (ECM). We have explored marrow ECM using mass spectrometry and found dermatopontin (DPT), a small non-collagenous ECM protein, to be present. We found that DPT cooperates with other ECM proteins to promote ...

journal_title：Stem cell reports

authors： Kramer AC,Blake AL,Taisto ME,Lehrke MJ,Webber BR,Lund TC

更新日期：2017-09-12 00:00:00

abstract：:Pluripotent cells emanate from the inner cell mass (ICM) of the blastocyst and when cultivated under optimal conditions immortalize as embryonic stem cells (ESCs). The fundamental mechanism underlying ESC derivation has, however, remained elusive. Recently, we have devised a highly efficient approach for establishing ...

journal_title：Stem cell reports

authors： Totonchi M,Hassani SN,Sharifi-Zarchi A,Tapia N,Adachi K,Arand J,Greber B,Sabour D,Araúzo-Bravo MJ,Walter J,Pakzad M,Gourabi H,Schöler HR,Baharvand H

更新日期：2017-10-10 00:00:00

abstract：:The transition from naive to primed state of pluripotent stem cells is hallmarked by epithelial-mesenchymal transition, metabolic switch from oxidative phosphorylation to aerobic glycolysis, and changes in the epigenetic landscape. Since these changes are also seen as putative hallmarks of neoplastic cell transformati...

journal_title：Stem cell reports

authors： Altshuler A,Verbuk M,Bhattacharya S,Abramovich I,Haklai R,Hanna JH,Kloog Y,Gottlieb E,Shalom-Feuerstein R

更新日期：2018-03-13 00:00:00

abstract：:High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically and functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained h...

journal_title：Stem cell reports

authors： Li J,Minami I,Shiozaki M,Yu L,Yajima S,Miyagawa S,Shiba Y,Morone N,Fukushima S,Yoshioka M,Li S,Qiao J,Li X,Wang L,Kotera H,Nakatsuji N,Sawa Y,Chen Y,Liu L

更新日期：2017-11-14 00:00:00

abstract：:Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 106 hiPSCs per layer yiel...

journal_title：Stem cell reports

authors： Tohyama S,Fujita J,Fujita C,Yamaguchi M,Kanaami S,Ohno R,Sakamoto K,Kodama M,Kurokawa J,Kanazawa H,Seki T,Kishino Y,Okada M,Nakajima K,Tanosaki S,Someya S,Hirano A,Kawaguchi S,Kobayashi E,Fukuda K

更新日期：2017-11-14 00:00:00

abstract：:Reappraisal of neuropathological studies suggests that pathological hallmarks of Alzheimer's disease and Parkinson's disease (PD) spread progressively along predictable neuronal pathways in the human brain through unknown mechanisms. Although there is much evidence supporting the prion-like propagation and amplificati...

journal_title：Stem cell reports

authors： Gribaudo S,Tixador P,Bousset L,Fenyi A,Lino P,Melki R,Peyrin JM,Perrier AL

更新日期：2019-02-12 00:00:00