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Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming.

Abstract：

:Reprogramming of somatic cells produces induced pluripotent stem cells (iPSCs) that are invaluable resources for biomedical research. Here, we extended the previous transcriptome studies by performing RNA-seq on cells defined by a combination of multiple cellular surface markers. We found that transcriptome changes during early reprogramming occur independently from the opening of closed chromatin by OCT4, SOX2, KLF4, and MYC (OSKM). Furthermore, our data identify multiple spliced forms of genes uniquely expressed at each progressive stage of reprogramming. In particular, we found a pluripotency-specific spliced form of CCNE1 that is specific to human and significantly enhances reprogramming. In addition, single nucleotide polymorphism (SNP) expression analysis reveals that monoallelic gene expression is induced in the intermediate stages of reprogramming, while biallelic expression is recovered upon completion of reprogramming. Our transcriptome data provide unique opportunities in understanding human iPSC reprogramming.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Tanaka Y,Hysolli E,Su J,Xiang Y,Kim KY,Zhong M,Li Y,Heydari K,Euskirchen G,Snyder MP,Pan X,Weissman SM,Park IH

doi

10.1016/j.stemcr.2015.04.009

subject

Has Abstract

pub_date

2015-06-09 00:00:00

pages

1125-39

issue

6

issn

2213-6711

pii

S2213-6711(15)00121-6

journal_volume

4

pub_type

杂志文章

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