Abstract:
:Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great promise for cardiac studies, but their structural and functional immaturity precludes their use as faithful models of adult myocardium. Here we describe engineered heart slices (EHS), preparations of decellularized porcine myocardium repopulated with hiPSC-CMs that exhibit structural and functional improvements over standard culture. EHS exhibited multicellular, aligned bundles of elongated CMs with organized sarcomeres, positive inotropic responses to isoproterenol, anisotropic conduction of action potentials, and electrophysiological functionality for more than 200 days. We developed a new drug assay, GRIDS, that serves as a "fingerprint" of cardiac drug sensitivity for a range of pacing rates and drug concentrations. GRIDS maps characterized differences in drug sensitivity between EHS and monolayers more clearly than changes in action potential durations or conduction velocities. EHS represent a tissue-like model for long-term culture, structural, and functional improvement, and higher fidelity drug response of hiPSC-CMs.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Blazeski A,Lowenthal J,Zhu R,Ewoldt J,Boheler KR,Tung Ldoi
10.1016/j.stemcr.2019.04.002subject
Has Abstractpub_date
2019-05-14 00:00:00pages
982-995issue
5issn
2213-6711pii
S2213-6711(19)30124-9journal_volume
12pub_type
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