Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury.

abstract：:Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulat...

journal_title：Stem cell reports

authors： Wang S,Wang X,Ma L,Lin X,Zhang D,Li Z,Wu Y,Zheng C,Feng X,Liao S,Feng Y,Chen J,Hu X,Wang M,Han C

更新日期：2016-07-12 00:00:00

abstract：:Human pluripotent stem cells are a valuable resource for transplantation, yet our ability to profile xenografts is largely limited to low-throughput immunohistochemical analysis by difficulties in readily isolating grafts for transcriptomic and/or proteomic profiling. Here, we present a simple methodology utilizing di...

journal_title：Stem cell reports

authors： Bye CR,Penna V,de Luzy IR,Gantner CW,Hunt CPJ,Thompson LH,Parish CL

更新日期：2019-11-12 00:00:00

abstract：:Human stem cell models have the potential to provide platforms for phenotypic screens to identify candidate treatments and cellular pathways involved in the pathogenesis of neurodegenerative disorders. Amyloid precursor protein (APP) processing and the accumulation of APP-derived amyloid β (Aβ) peptides are key proces...

journal_title：Stem cell reports

authors： Brownjohn PW,Smith J,Portelius E,Serneels L,Kvartsberg H,De Strooper B,Blennow K,Zetterberg H,Livesey FJ

更新日期：2017-04-11 00:00:00

abstract：:Non-human primates (NHPs) can serve as a human-like model to study cell therapy using induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). However, whether the efficacy of NHP and human iPSC-CMs is mechanistically similar remains unknown. To examine this, RNU rats received intramyocardial injection of 1 × ...

journal_title：Stem cell reports

authors： Zhao X,Chen H,Xiao D,Yang H,Itzhaki I,Qin X,Chour T,Aguirre A,Lehmann K,Kim Y,Shukla P,Holmström A,Zhang JZ,Zhuge Y,Ndoye BC,Zhao M,Neofytou E,Zimmermann WH,Jain M,Wu JC

更新日期：2018-02-13 00:00:00

abstract：:There are currently hundreds of businesses across the United States offering direct-to-consumer stem cell treatments that have not been through regulatory approval by the Food and Drug Administration (FDA). Here, we provide a detailed characterization of nearly 170 stem cell businesses operating in the Southwest Unite...

journal_title：Stem cell reports

authors： Frow EK,Brafman DA,Muldoon A,Krum L,Williams P,Becker B,Nelson JP,Pritchett A

更新日期：2019-08-13 00:00:00

abstract：:Hematopoietic stem cells (HSCs) exist in a dormant state and progressively lose regenerative potency as they undergo successive divisions. Why this functional decline occurs and how this information is encoded is unclear. To better understand how this information is stored, we performed RNA sequencing on HSC populatio...

journal_title：Stem cell reports

authors： Bernitz JM,Rapp K,Daniel MG,Shcherbinin D,Yuan Y,Gomes A,Waghray A,Brosh R,Lachmann A,Ma'ayan A,Papatsenko D,Moore KA

更新日期：2020-04-14 00:00:00

abstract：:When comparing hepatic phenotypes between iPSC-derived hepatocyte-like cells from different liver disease patients, cell heterogeneity can confound interpretation. We proposed that homogeneous cell populations could be generated by fluorescence-activated cell sorting (FACS). Using cell-surface capture proteomics, we i...

journal_title：Stem cell reports

authors： Mallanna SK,Cayo MA,Twaroski K,Gundry RL,Duncan SA

更新日期：2016-09-13 00:00:00

abstract：:The in vitro derivation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) is complicated by the existence of multiple overlapping embryonic blood cell programs called primitive, erythromyeloid progenitor (EMP), and definitive. As HSCs are only generated during the definitive stage of hematopoiesis,...

journal_title：Stem cell reports

authors： Clarke RL,Robitaille AM,Moon RT,Keller G

更新日期：2015-08-11 00:00:00

abstract：:Precise methods for transgene regulation are important to study signaling pathways and cell lineages in biological systems where gene function is often recycled within and across lineages. We engineered a genetic toolset for flexible transgene regulation in these diverse cellular contexts. Specifically, we created an ...

journal_title：Stem cell reports

authors： Akhtar AA,Molina J,Dutra-Clarke M,Kim GB,Levy R,Schreiber-Stainthorp W,Danielpour M,Breunig JJ

更新日期：2015-03-10 00:00:00

abstract：:The transcription factor (TF) GATA2 plays a key role in organ development and cell fate control in the central nervous, urogenital, respiratory, and reproductive systems, and in primitive and definitive hematopoiesis. Here, we generate a knockin protein reporter mouse line expressing a GATA2VENUS fusion from the endog...

journal_title：Stem cell reports

authors： Ahmed N,Kunz L,Hoppe PS,Loeffler D,Etzrodt M,Ortega GC,Hilsenbeck O,Anastassiadis K,Schroeder T

更新日期：2020-08-11 00:00:00

abstract：:Deletion of caudal/cdx genes alters hox gene expression and causes defects in posterior tissues and hematopoiesis. Yet, the defects in hox gene expression only partially explain these phenotypes. To gain deeper insight into Cdx4 function, we performed chromatin immunoprecipitation sequencing (ChIP-seq) combined with g...

journal_title：Stem cell reports

authors： Paik EJ,Mahony S,White RM,Price EN,Dibiase A,Dorjsuren B,Mosimann C,Davidson AJ,Gifford D,Zon LI

更新日期：2013-11-07 00:00:00

abstract：:The microenvironment is a critical mediator of stem cell survival, proliferation, migration, and differentiation. The majority of preclinical studies involving transplantation of neural stem cells (NSCs) into the CNS have focused on injured or degenerating microenvironments, leaving a dearth of information as to how N...

journal_title：Stem cell reports

authors： Sontag CJ,Uchida N,Cummings BJ,Anderson AJ

更新日期：2014-04-18 00:00:00

abstract：:We identified osteoclast defects in craniometaphyseal dysplasia (CMD) using an easy-to-use protocol for differentiating osteoclasts from human induced pluripotent stem cells (hiPSCs). CMD is a rare genetic bone disorder, characterized by life-long progressive thickening of craniofacial bones and abnormal shape of long...

journal_title：Stem cell reports

authors： Chen IP,Luxmi R,Kanaujiya J,Hao Z,Reichenberger EJ

更新日期：2017-11-14 00:00:00

abstract：:Thyroid hormone (TH) transporters are required for the transmembrane passage of TH in target cells. In humans, inactivating mutations in the TH transporter MCT8 cause the Allan-Herndon-Dudley syndrome, characterized by severe neuromuscular symptoms and an abnormal TH serum profile, which is fully replicated in Mct8 kn...

journal_title：Stem cell reports

authors： Mayerl S,Schmidt M,Doycheva D,Darras VM,Hüttner SS,Boelen A,Visser TJ,Kaether C,Heuer H,von Maltzahn J

更新日期：2018-06-05 00:00:00

abstract：:Pluripotent stem cells have been proposed as an unlimited source of pancreatic β cells for studying and treating diabetes. However, the long, multi-step differentiation protocols used to generate functional β cells inevitably exhibit considerable variability, particularly when applied to pluripotent cells from diverse...

journal_title：Stem cell reports

authors： Trott J,Tan EK,Ong S,Titmarsh DM,Denil SLIJ,Giam M,Wong CK,Wang J,Shboul M,Eio M,Cooper-White J,Cool SM,Rancati G,Stanton LW,Reversade B,Dunn NR

更新日期：2017-06-06 00:00:00

abstract：:Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using...

journal_title：Stem cell reports

authors： Sugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi M

更新日期：2017-11-14 00:00:00

abstract：:Pluripotent cells emanate from the inner cell mass (ICM) of the blastocyst and when cultivated under optimal conditions immortalize as embryonic stem cells (ESCs). The fundamental mechanism underlying ESC derivation has, however, remained elusive. Recently, we have devised a highly efficient approach for establishing ...

journal_title：Stem cell reports

authors： Totonchi M,Hassani SN,Sharifi-Zarchi A,Tapia N,Adachi K,Arand J,Greber B,Sabour D,Araúzo-Bravo MJ,Walter J,Pakzad M,Gourabi H,Schöler HR,Baharvand H

更新日期：2017-10-10 00:00:00

abstract：:Generation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) could potentially provide unlimited HSCs for clinical transplantation, a curative treatment for numerous blood diseases. However, to date, bona fide HSC generation has been largely unsuccessful in vitro. We have previously described proof...

journal_title：Stem cell reports

authors： Tsukada M,Ota Y,Wilkinson AC,Becker HJ,Osato M,Nakauchi H,Yamazaki S

更新日期：2017-10-10 00:00:00

abstract：:Genetically engineered human pluripotent stem cells (hPSCs) have been proposed as a source for transplantation therapies and are rapidly becoming valuable tools for human disease modeling. However, many applications are limited due to the lack of robust differentiation paradigms that allow for the isolation of defined...

journal_title：Stem cell reports

authors： Forster R,Chiba K,Schaeffer L,Regalado SG,Lai CS,Gao Q,Kiani S,Farin HF,Clevers H,Cost GJ,Chan A,Rebar EJ,Urnov FD,Gregory PD,Pachter L,Jaenisch R,Hockemeyer D

更新日期：2014-06-03 00:00:00

abstract：:Primary tauopathies are characterized neuropathologically by inclusions containing abnormal forms of the microtubule-associated protein tau (MAPT) and clinically by diverse neuropsychiatric, cognitive, and motor impairments. Autosomal dominant mutations in the MAPT gene cause heterogeneous forms of frontotemporal loba...

journal_title：Stem cell reports

authors： Karch CM,Kao AW,Karydas A,Onanuga K,Martinez R,Argouarch A,Wang C,Huang C,Sohn PD,Bowles KR,Spina S,Silva MC,Marsh JA,Hsu S,Pugh DA,Ghoshal N,Norton J,Huang Y,Lee SE,Seeley WW,Theofilas P,Grinberg LT,Moreno F

更新日期：2019-11-12 00:00:00

abstract：:Robust production of terminally differentiated cells from self-renewing resident stem cells is essential to maintain proper tissue architecture and physiological functions, especially in high-turnover tissues. However, the transcriptional networks that precisely regulate cell transition and differentiation are poorly ...

journal_title：Stem cell reports

authors： Meng FW,Rojas Villa SE,Biteau B

更新日期：2020-02-11 00:00:00

abstract：:Developing effective therapeutics for complex diseases such as late-onset, sporadic Alzheimer's disease (SAD) is difficult due to genetic and environmental heterogeneity in the human population and the limitations of existing animal models. Here, we used hiPSC-derived neurons to test a compound that stabilizes the ret...

journal_title：Stem cell reports

authors： Young JE,Fong LK,Frankowski H,Petsko GA,Small SA,Goldstein LSB

更新日期：2018-03-13 00:00:00

abstract：:Mesenchymal stem cells (MSCs) can donate mitochondria and rescue anthracycline-induced cardiomyocyte (CM) damage, although the underlying mechanisms remain elusive. We determined that the superior efficiency of mitochondrial transfer by human induced-pluripotent-stem-cell-derived MSCs (iPSC-MSCs) compared with bone ma...

journal_title：Stem cell reports

authors： Zhang Y,Yu Z,Jiang D,Liang X,Liao S,Zhang Z,Yue W,Li X,Chiu SM,Chai YH,Liang Y,Chow Y,Han S,Xu A,Tse HF,Lian Q

更新日期：2016-10-11 00:00:00

abstract：:Research in human induced pluripotent stem cells (hiPSCs) is rapidly developing and there are expectations that this research may obviate the need to use human embryonic stem cells (hESCs), the ethics of which has been a subject of controversy for more than 15 years. In this study, we investigated approximately 3,400 ...

journal_title：Stem cell reports

authors： Kobold S,Guhr A,Kurtz A,Löser P

更新日期：2015-05-12 00:00:00

abstract：:Nerve-derived neural crest cells are essential for regeneration in certain animals, such as newts. Here, we asked whether they play a similar role during mammalian tissue repair, focusing on Sox2-positive neural crest precursors in skin. In adult skin, Sox2 was expressed in nerve-terminal-associated neural crest precu...

journal_title：Stem cell reports

authors： Johnston AP,Naska S,Jones K,Jinno H,Kaplan DR,Miller FD

更新日期：2013-06-04 00:00:00

abstract：:Interleukin-6 (IL-6) is increased in maternal serum and amniotic fluid of children subsequently diagnosed with autism spectrum disorders. However, it is not clear how increased IL-6 alters brain development. Here, we show that IL-6 increases the prevalence of a specific platelet-derived growth factor (PDGF)-responsive...

journal_title：Stem cell reports

authors： Kumari E,Velloso FJ,Nasuhidehnavi A,Somasundaram A,Savanur VH,Buono KD,Levison SW

更新日期：2020-05-12 00:00:00

abstract：:Fanconi anemia (FA) causes bone marrow failure early during childhood, and recent studies indicate that a hematopoietic defect could begin in utero. We performed a unique kinetics study of hematopoiesis in Fancg-/- mouse embryos, between the early embryonic day 11.5 (E11.5) to E12.5 developmental window (when the high...

journal_title：Stem cell reports

authors： Domenech C,Maillard L,Rousseau A,Guidez F,Petit L,Pla M,Clay D,Guimiot F,Sanfilippo S,Jacques S,de la Grange P,Robil N,Soulier J,Souyri M

更新日期：2018-11-13 00:00:00

abstract：:Teratoma formation is the gold standard assay for testing the capacity of human pluripotent stem cells to differentiate into all embryonic germ layers. Although widely used, little effort has been made to transform this qualitative assay into a quantitative one. Using gene expression data from a wide variety of cells,...

journal_title：Stem cell reports

authors： Avior Y,Biancotti JC,Benvenisty N

更新日期：2015-06-09 00:00:00

abstract：:Microglia, the immune cells of the brain, are crucial to proper development and maintenance of the CNS, and their involvement in numerous neurological disorders is increasingly being recognized. To improve our understanding of human microglial biology, we devised a chemically defined protocol to generate human microgl...

journal_title：Stem cell reports

authors： Douvaras P,Sun B,Wang M,Kruglikov I,Lallos G,Zimmer M,Terrenoire C,Zhang B,Gandy S,Schadt E,Freytes DO,Noggle S,Fossati V

更新日期：2017-06-06 00:00:00

abstract：:In human-to-mouse xenograft models, reconstitution of human hematopoiesis is usually B-lymphoid dominant. Here we show that the introduction of homozygous Kit(Wv) mutations into C57BL/6.Rag2(null)Il2rg(null) mice with NOD-Sirpa (BRGS) strongly promoted human multi-lineage reconstitution. After xenotransplantation of h...

journal_title：Stem cell reports

authors： Yurino A,Takenaka K,Yamauchi T,Nunomura T,Uehara Y,Jinnouchi F,Miyawaki K,Kikushige Y,Kato K,Miyamoto T,Iwasaki H,Kunisaki Y,Akashi K

更新日期：2016-09-13 00:00:00