当前位置： SCI文献检索 > Stem Cell Reports期刊下所有文献 > High-Level Precise Knockin of iPSCs by Simultaneous Reprogramming and Genome Editing of Human Peripheral Blood Mononuclear Cells.

High-Level Precise Knockin of iPSCs by Simultaneous Reprogramming and Genome Editing of Human Peripheral Blood Mononuclear Cells.

Abstract：

:We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use of an optimized CRISPR-Cas9 system together with a double-cut donor increases homology-directed repair-mediated precise gene knockin efficiency by 5- to 10-fold. Here, we report the integration of blood cell reprogramming and genome editing in a single step. We found that expression of Cas9 and KLF4 using a single vector significantly increases genome editing efficiency, and addition of SV40LT further enhances knockin efficiency. After these optimizations, genome editing efficiency of up to 40% in the bulk iPSC population can be achieved without any selection. Most of the edited cells show characteristics of iPSCs and genome integrity. Our improved approach, which integrates reprogramming and genome editing, should expedite both basic research and clinical applications of precision and regenerative medicine.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Wen W,Cheng X,Fu Y,Meng F,Zhang JP,Zhang L,Li XL,Yang Z,Xu J,Zhang F,Botimer GD,Yuan W,Sun C,Cheng T,Zhang XB

doi

10.1016/j.stemcr.2018.04.013

subject

Has Abstract

pub_date

2018-06-05 00:00:00

pages

1821-1834

issue

6

issn

2213-6711

pii

S2213-6711(18)30181-4

journal_volume

10

pub_type

杂志文章

相关文献

Stem Cell Reports文献大全
  • Adult Neural Progenitor Cells Transplanted into Spinal Cord Injury Differentiate into Oligodendrocytes, Enhance Myelination, and Contribute to Recovery.

    abstract：:Long-term survival and integration of neural progenitor cells (NPCs) transplanted following spinal cord injury (SCI) have been observed. However, questions concerning the differentiation choice, the mechanism of action, and the contribution of NPCs to functional recovery remains unanswered. Therefore, we investigated ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.03.013

    authors： Sankavaram SR,Hakim R,Covacu R,Frostell A,Neumann S,Svensson M,Brundin L

    更新日期：2019-05-14 00:00:00

  • A Novel GATA2 Protein Reporter Mouse Reveals Hematopoietic Progenitor Cell Types.

    abstract：:The transcription factor (TF) GATA2 plays a key role in organ development and cell fate control in the central nervous, urogenital, respiratory, and reproductive systems, and in primitive and definitive hematopoiesis. Here, we generate a knockin protein reporter mouse line expressing a GATA2VENUS fusion from the endog...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.06.008

    authors： Ahmed N,Kunz L,Hoppe PS,Loeffler D,Etzrodt M,Ortega GC,Hilsenbeck O,Anastassiadis K,Schroeder T

    更新日期：2020-08-11 00:00:00

  • Rapid Crypt Cell Remodeling Regenerates the Intestinal Stem Cell Niche after Notch Inhibition.

    abstract：:Intestinal crypts have great capacity for repair and regeneration after intestinal stem cell (ISC) injury. Here, we define the cellular remodeling process resulting from ISC niche interruption by transient Notch pathway inhibition in adult mice. Although ISCs were retained, lineage tracing demonstrated a marked reduct...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.05.010

    authors： Bohin N,Keeley TM,Carulli AJ,Walker EM,Carlson EA,Gao J,Aifantis I,Siebel CW,Rajala MW,Myers MG Jr,Jones JC,Brindley CD,Dempsey PJ,Samuelson LC

    更新日期：2020-07-14 00:00:00

  • PGC Reversion to Pluripotency Involves Erasure of DNA Methylation from Imprinting Control Centers followed by Locus-Specific Re-methylation.

    abstract：:Primordial germ cells (PGCs) are fate restricted to differentiate into gametes in vivo. However, when removed from their embryonic niche, PGCs undergo reversion to pluripotent embryonic germ cells (EGCs) in vitro. One of the major differences between EGCs and embryonic stem cells (ESCs) is variable methylation at impr...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.07.006

    authors： Oliveros-Etter M,Li Z,Nee K,Hosohama L,Hargan-Calvopina J,Lee SA,Joti P,Yu J,Clark AT

    更新日期：2015-09-08 00:00:00

  • Mapping the Cell-Surface N-Glycoproteome of Human Hepatocytes Reveals Markers for Selecting a Homogeneous Population of iPSC-Derived Hepatocytes.

    abstract：:When comparing hepatic phenotypes between iPSC-derived hepatocyte-like cells from different liver disease patients, cell heterogeneity can confound interpretation. We proposed that homogeneous cell populations could be generated by fluorescence-activated cell sorting (FACS). Using cell-surface capture proteomics, we i...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.07.016

    authors： Mallanna SK,Cayo MA,Twaroski K,Gundry RL,Duncan SA

    更新日期：2016-09-13 00:00:00

  • LNGFR(+)THY-1(+)VCAM-1(hi+) cells reveal functionally distinct subpopulations in mesenchymal stem cells.

    abstract：:Human mesenchymal stem cells (hMSCs), which conventionally are isolated based on their adherence to plastic, are heterogeneous and have poor growth and differentiation, limiting our ability to investigate their intrinsic characteristics. We report an improved prospective clonal isolation technique and reveal that the ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.06.001

    authors： Mabuchi Y,Morikawa S,Harada S,Niibe K,Suzuki S,Renault-Mihara F,Houlihan DD,Akazawa C,Okano H,Matsuzaki Y

    更新日期：2013-07-11 00:00:00

  • Direct reprogramming of human fibroblasts toward a cardiomyocyte-like state.

    abstract：:Direct reprogramming of adult somatic cells into alternative cell types has been shown for several lineages. We previously showed that GATA4, MEF2C, and TBX5 (GMT) directly reprogrammed nonmyocyte mouse heart cells into induced cardiomyocyte-like cells (iCMs) in vitro and in vivo. However, GMT alone appears insufficie...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2013.07.005

    authors： Fu JD,Stone NR,Liu L,Spencer CI,Qian L,Hayashi Y,Delgado-Olguin P,Ding S,Bruneau BG,Srivastava D

    更新日期：2013-08-22 00:00:00

  • FAM122A Inhibits Erythroid Differentiation through GATA1.

    abstract：:FAM122A is a highly conserved housekeeping gene, but its physiological and pathophysiological roles remain greatly elusive. Based on the fact that FAM122A is highly expressed in human CD71+ early erythroid cells, herein we report that FAM122A is downregulated during erythroid differentiation, while its overexpression ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.07.010

    authors： Chen J,Zhou Q,Liu MH,Yang YS,Wang YQ,Huang Y,Chen GQ

    更新日期：2020-09-08 00:00:00

  • Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke.

    abstract：:Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate new neurons capable of func...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.10.011

    authors： Kannangara TS,Carter A,Xue Y,Dhaliwal JS,Béïque JC,Lagace DC

    更新日期：2018-12-11 00:00:00

  • Five transcription factors and FGF pathway inhibition efficiently induce erythroid differentiation in the epiblast.

    abstract：:Primitive erythropoiesis follows a stereotypic developmental program of mesoderm ventralization and internalization, hemangioblast formation and migration, and erythroid lineage specification. Induction of erythropoiesis is inefficient in either ES/iPS cells in vitro or nonhemangioblast cell populations in vivo. Using...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2014.01.019

    authors： Weng W,Sheng G

    更新日期：2014-03-06 00:00:00

  • Function of Arl4aa in the Initiation of Hematopoiesis in Zebrafish by Maintaining Golgi Complex Integrity in Hemogenic Endothelium.

    abstract：:ADP-ribosylation factor-like 4aa (Arl4aa) is a member of the ADP-ribosylation factor family. It is expressed in hematopoietic tissue during embryonic development, but its function was unknown. Zebrafish arl4aa is preferentially expressed in the ventral wall of the dorsal aorta (VDA) at 24 and 36 hpf and in caudal hema...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2020.02.012

    authors： Guo Y,Cheng BYL,Wang D,Ma ACH,He BL,Man TK,Cheung MPL,Shi X,Ng NKL,Leung AYH

    更新日期：2020-04-14 00:00:00

  • Functional Properties of Engineered Heart Slices Incorporating Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

    abstract：:Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great promise for cardiac studies, but their structural and functional immaturity precludes their use as faithful models of adult myocardium. Here we describe engineered heart slices (EHS), preparations of decellularized porcine myocardium rep...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2019.04.002

    authors： Blazeski A,Lowenthal J,Zhu R,Ewoldt J,Boheler KR,Tung L

    更新日期：2019-05-14 00:00:00

  • An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs.

    abstract：:Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput ima...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.08.023

    authors： Larribère L,Galach M,Novak D,Arévalo K,Volz HC,Stark HJ,Boukamp P,Boutros M,Utikal J

    更新日期：2017-10-10 00:00:00

  • Differentiation-Defective Human Induced Pluripotent Stem Cells Reveal Strengths and Limitations of the Teratoma Assay and In Vitro Pluripotency Assays.

    abstract：:The ability to form teratomas in vivo containing multiple somatic cell types is regarded as functional evidence of pluripotency for human pluripotent stem cells (hPSCs). Since the Teratoma assay is animal dependent, laborious, and only qualitative, the PluriTest and the hPSC ScoreCard assay have been developed as in v...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.03.009

    authors： Bouma MJ,van Iterson M,Janssen B,Mummery CL,Salvatori DCF,Freund C

    更新日期：2017-05-09 00:00:00

  • Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development.

    abstract：:Human stem cell-derived models of development and neurodegenerative diseases are challenged by cellular immaturity in vitro. Microengineered organ-on-chip (or Organ-Chip) systems are designed to emulate microvolume cytoarchitecture and enable co-culture of distinct cell types. Brain microvascular endothelial cells (BM...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.02.012

    authors： Sances S,Ho R,Vatine G,West D,Laperle A,Meyer A,Godoy M,Kay PS,Mandefro B,Hatata S,Hinojosa C,Wen N,Sareen D,Hamilton GA,Svendsen CN

    更新日期：2018-04-10 00:00:00

  • Transplanted Donor- or Stem Cell-Derived Cone Photoreceptors Can Both Integrate and Undergo Material Transfer in an Environment-Dependent Manner.

    abstract：:Human vision relies heavily upon cone photoreceptors, and their loss results in permanent visual impairment. Transplantation of healthy photoreceptors can restore visual function in models of inherited blindness, a process previously understood to arise by donor cell integration within the host retina. However, we and...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.12.008

    authors： Waldron PV,Di Marco F,Kruczek K,Ribeiro J,Graca AB,Hippert C,Aghaizu ND,Kalargyrou AA,Barber AC,Grimaldi G,Duran Y,Blackford SJI,Kloc M,Goh D,Zabala Aldunate E,Sampson RD,Bainbridge JWB,Smith AJ,Gonzalez-Cordero A,S

    更新日期：2018-02-13 00:00:00

  • Enhanced Reconstitution of Human Erythropoiesis and Thrombopoiesis in an Immunodeficient Mouse Model with Kit(Wv) Mutations.

    abstract：:In human-to-mouse xenograft models, reconstitution of human hematopoiesis is usually B-lymphoid dominant. Here we show that the introduction of homozygous Kit(Wv) mutations into C57BL/6.Rag2(null)Il2rg(null) mice with NOD-Sirpa (BRGS) strongly promoted human multi-lineage reconstitution. After xenotransplantation of h...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.07.002

    authors： Yurino A,Takenaka K,Yamauchi T,Nunomura T,Uehara Y,Jinnouchi F,Miyawaki K,Kikushige Y,Kato K,Miyamoto T,Iwasaki H,Kunisaki Y,Akashi K

    更新日期：2016-09-13 00:00:00

  • Structural phenotyping of stem cell-derived cardiomyocytes.

    abstract：:Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.01.020

    authors： Pasqualini FS,Sheehy SP,Agarwal A,Aratyn-Schaus Y,Parker KK

    更新日期：2015-03-10 00:00:00

  • ER Stress and Autophagic Perturbations Lead to Elevated Extracellular α-Synuclein in GBA-N370S Parkinson's iPSC-Derived Dopamine Neurons.

    abstract：:Heterozygous mutations in the glucocerebrosidase gene (GBA) represent the strongest common genetic risk factor for Parkinson's disease (PD), the second most common neurodegenerative disorder. However, the molecular mechanisms underlying this association are still poorly understood. Here, we have analyzed ten independe...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.01.013

    authors： Fernandes HJ,Hartfield EM,Christian HC,Emmanoulidou E,Zheng Y,Booth H,Bogetofte H,Lang C,Ryan BJ,Sardi SP,Badger J,Vowles J,Evetts S,Tofaris GK,Vekrellis K,Talbot K,Hu MT,James W,Cowley SA,Wade-Martins R

    更新日期：2016-03-08 00:00:00

  • Propagation of α-Synuclein Strains within Human Reconstructed Neuronal Network.

    abstract：:Reappraisal of neuropathological studies suggests that pathological hallmarks of Alzheimer's disease and Parkinson's disease (PD) spread progressively along predictable neuronal pathways in the human brain through unknown mechanisms. Although there is much evidence supporting the prion-like propagation and amplificati...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.12.007

    authors： Gribaudo S,Tixador P,Bousset L,Fenyi A,Lino P,Melki R,Peyrin JM,Perrier AL

    更新日期：2019-02-12 00:00:00

  • Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming.

    abstract：:Reprogramming of somatic cells produces induced pluripotent stem cells (iPSCs) that are invaluable resources for biomedical research. Here, we extended the previous transcriptome studies by performing RNA-seq on cells defined by a combination of multiple cellular surface markers. We found that transcriptome changes du...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.04.009

    authors： Tanaka Y,Hysolli E,Su J,Xiang Y,Kim KY,Zhong M,Li Y,Heydari K,Euskirchen G,Snyder MP,Pan X,Weissman SM,Park IH

    更新日期：2015-06-09 00:00:00

  • Histone H3 Lysine 9 Acetylation Obstructs ATM Activation and Promotes Ionizing Radiation Sensitivity in Normal Stem Cells.

    abstract：:Dynamic spatiotemporal modification of chromatin around DNA damage is vital for efficient DNA repair. Normal stem cells exhibit an attenuated DNA damage response (DDR), inefficient DNA repair, and high radiosensitivity. The impact of unique chromatin characteristics of stem cells in DDR regulation is not yet recognize...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.11.004

    authors： Meyer B,Fabbrizi MR,Raj S,Zobel CL,Hallahan DE,Sharma GG

    更新日期：2016-12-13 00:00:00

  • Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells.

    abstract：:Pro-fibrotic microenvironments of scars and tumors characterized by increased stiffness stimulate mesenchymal stromal cells (MSCs) to express α-smooth muscle actin (α-SMA). We investigated whether incorporation of α-SMA into contractile stress fibers regulates human MSC fate. Sorted α-SMA-positive MSCs exhibited high ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.05.004

    authors： Talele NP,Fradette J,Davies JE,Kapus A,Hinz B

    更新日期：2015-06-09 00:00:00

  • Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System.

    abstract：:Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nerv...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.05.010

    authors： Rigamonti A,Repetti GG,Sun C,Price FD,Reny DC,Rapino F,Weisinger K,Benkler C,Peterson QP,Davidow LS,Hansson EM,Rubin LL

    更新日期：2016-06-14 00:00:00

  • HDAC1 and HDAC2 Modulate TGF-β Signaling during Endothelial-to-Hematopoietic Transition.

    abstract：:The first hematopoietic stem and progenitor cells are generated during development from hemogenic endothelium (HE) through trans-differentiation. The molecular mechanisms underlying this endothelial-to-hematopoietic transition (EHT) remain poorly understood. Here, we explored the role of the epigenetic regulators HDAC...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.03.011

    authors： Thambyrajah R,Fadlullah MZH,Proffitt M,Patel R,Cowley SM,Kouskoff V,Lacaud G

    更新日期：2018-04-10 00:00:00

  • GATA6 Cooperates with EOMES/SMAD2/3 to Deploy the Gene Regulatory Network Governing Human Definitive Endoderm and Pancreas Formation.

    abstract：:Heterozygous de novo mutations in GATA6 are the most frequent cause of pancreatic agenesis in humans. In mice, however, a similar phenotype requires the biallelic loss of Gata6 and its paralog Gata4. To elaborate the human-specific requirements for GATA6, we chose to model GATA6 loss in vitro by combining both gene-ed...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.12.003

    authors： Chia CY,Madrigal P,Denil SLIJ,Martinez I,Garcia-Bernardo J,El-Khairi R,Chhatriwala M,Shepherd MH,Hattersley AT,Dunn NR,Vallier L

    更新日期：2019-01-08 00:00:00

  • LincU Preserves Naïve Pluripotency by Restricting ERK Activity in Embryonic Stem Cells.

    abstract：:Although the functional roles of long noncoding RNAs (lncRNAs) have been increasingly identified, few lncRNAs that control the naïve state of embryonic stem cells (ESCs) are known. Here, we report a naïve-state-associated lncRNA, LincU, which is intrinsically activated by Nanog in mESCs. LincU-deficient mESCs exhibit ...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2018.06.010

    authors： Jiapaer Z,Li G,Ye D,Bai M,Li J,Guo X,Du Y,Su D,Jia W,Chen W,Wang G,Yu Y,Zhu F,Wan X,Kang J

    更新日期：2018-08-14 00:00:00

  • TeratoScore: Assessing the Differentiation Potential of Human Pluripotent Stem Cells by Quantitative Expression Analysis of Teratomas.

    abstract：:Teratoma formation is the gold standard assay for testing the capacity of human pluripotent stem cells to differentiate into all embryonic germ layers. Although widely used, little effort has been made to transform this qualitative assay into a quantitative one. Using gene expression data from a wide variety of cells,...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2015.05.006

    authors： Avior Y,Biancotti JC,Benvenisty N

    更新日期：2015-06-09 00:00:00

  • Reciprocal Regulation between Bifunctional miR-9/9(∗) and its Transcriptional Modulator Notch in Human Neural Stem Cell Self-Renewal and Differentiation.

    abstract：:Tight regulation of the balance between self-renewal and differentiation of neural stem cells is crucial to assure proper neural development. In this context, Notch signaling is a well-known promoter of stemness. In contrast, the bifunctional brain-enriched microRNA miR-9/9(∗) has been implicated in promoting neuronal...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2016.06.008

    authors： Roese-Koerner B,Stappert L,Berger T,Braun NC,Veltel M,Jungverdorben J,Evert BO,Peitz M,Borghese L,Brüstle O

    更新日期：2016-08-09 00:00:00

  • Context-Dependent Functions of NANOG Phosphorylation in Pluripotency and Reprogramming.

    abstract：:The core pluripotency transcription factor NANOG is critical for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Although NANOG is phosphorylated at multiple residues, the role of NANOG phosphorylation in ESC self-renewal is incompletely understood, and no information exists regarding its functi...

    journal_title：Stem cell reports

    pub_type： 杂志文章

    doi：10.1016/j.stemcr.2017.03.023

    authors： Saunders A,Li D,Faiola F,Huang X,Fidalgo M,Guallar D,Ding J,Yang F,Xu Y,Zhou H,Wang J

    更新日期：2017-05-09 00:00:00