An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs.

abstract：:A period of mild brain overgrowth with an unknown etiology has been identified as one of the most common phenotypes in autism. Here, we test the hypothesis that maternal inflammation during critical periods of embryonic development can cause brain overgrowth and autism-associated behaviors as a result of altered neura...

journal_title：Stem cell reports

authors： Le Belle JE,Sperry J,Ngo A,Ghochani Y,Laks DR,López-Aranda M,Silva AJ,Kornblum HI

更新日期：2014-11-11 00:00:00

abstract：:Generation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) could potentially provide unlimited HSCs for clinical transplantation, a curative treatment for numerous blood diseases. However, to date, bona fide HSC generation has been largely unsuccessful in vitro. We have previously described proof...

journal_title：Stem cell reports

authors： Tsukada M,Ota Y,Wilkinson AC,Becker HJ,Osato M,Nakauchi H,Yamazaki S

更新日期：2017-10-10 00:00:00

abstract：:Alzheimer's disease (AD) induces memory and cognitive impairment in the absence of motor and sensory deficits during its early and middle course. A major unresolved question is the basis for this selective neuronal vulnerability. Aβ, which plays a central role in AD pathogenesis, is generated throughout the brain, yet...

journal_title：Stem cell reports

authors： Muratore CR,Zhou C,Liao M,Fernandez MA,Taylor WM,Lagomarsino VN,Pearse RV 2nd,Rice HC,Negri JM,He A,Srikanth P,Callahan DG,Shin T,Zhou M,Bennett DA,Noggle S,Love JC,Selkoe DJ,Young-Pearse TL

更新日期：2017-12-12 00:00:00

abstract：:Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nerv...

journal_title：Stem cell reports

authors： Rigamonti A,Repetti GG,Sun C,Price FD,Reny DC,Rapino F,Weisinger K,Benkler C,Peterson QP,Davidow LS,Hansson EM,Rubin LL

更新日期：2016-06-14 00:00:00

abstract：:The rigorous characterization of distinct induced pluripotent stem cells (iPSC) derived from multiple reprogramming technologies, somatic sources, and donors is required to understand potential sources of variability and downstream potential. To achieve this goal, the Progenitor Cell Biology Consortium performed compr...

journal_title：Stem cell reports

authors： Salomonis N,Dexheimer PJ,Omberg L,Schroll R,Bush S,Huo J,Schriml L,Ho Sui S,Keddache M,Mayhew C,Shanmukhappa SK,Wells J,Daily K,Hubler S,Wang Y,Zambidis E,Margolin A,Hide W,Hatzopoulos AK,Malik P,Cancelas JA,Aro

更新日期：2016-07-12 00:00:00

abstract：:Intestinal crypts have great capacity for repair and regeneration after intestinal stem cell (ISC) injury. Here, we define the cellular remodeling process resulting from ISC niche interruption by transient Notch pathway inhibition in adult mice. Although ISCs were retained, lineage tracing demonstrated a marked reduct...

journal_title：Stem cell reports

authors： Bohin N,Keeley TM,Carulli AJ,Walker EM,Carlson EA,Gao J,Aifantis I,Siebel CW,Rajala MW,Myers MG Jr,Jones JC,Brindley CD,Dempsey PJ,Samuelson LC

更新日期：2020-07-14 00:00:00

abstract：:Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate new neurons capable of func...

journal_title：Stem cell reports

authors： Kannangara TS,Carter A,Xue Y,Dhaliwal JS,Béïque JC,Lagace DC

更新日期：2018-12-11 00:00:00

abstract：:Transcriptome analysis has uncovered a series of long noncoding RNAs (lncRNAs) transcribed during cell differentiation, but how lncRNA is integrated with known transcriptional regulatory network is poorly understood. Here, we utilize human definitive endoderm differentiation as a model system and decipher the function...

journal_title：Stem cell reports

authors： Yang J,Lu P,Li M,Yan C,Zhang T,Jiang W

更新日期：2020-09-08 00:00:00

abstract：:The ability to form teratomas in vivo containing multiple somatic cell types is regarded as functional evidence of pluripotency for human pluripotent stem cells (hPSCs). Since the Teratoma assay is animal dependent, laborious, and only qualitative, the PluriTest and the hPSC ScoreCard assay have been developed as in v...

journal_title：Stem cell reports

authors： Bouma MJ,van Iterson M,Janssen B,Mummery CL,Salvatori DCF,Freund C

更新日期：2017-05-09 00:00:00

abstract：:Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 106 hiPSCs per layer yiel...

journal_title：Stem cell reports

authors： Tohyama S,Fujita J,Fujita C,Yamaguchi M,Kanaami S,Ohno R,Sakamoto K,Kodama M,Kurokawa J,Kanazawa H,Seki T,Kishino Y,Okada M,Nakajima K,Tanosaki S,Someya S,Hirano A,Kawaguchi S,Kobayashi E,Fukuda K

更新日期：2017-11-14 00:00:00

abstract：:Genetic changes in human pluripotent stem cells (hPSCs) gained during culture can confound experimental results and potentially jeopardize the outcome of clinical therapies. Particularly common changes in hPSCs are trisomies of chromosomes 1, 12, 17, and 20. Thus, hPSCs should be regularly screened for such aberration...

journal_title：Stem cell reports

authors： Baker D,Hirst AJ,Gokhale PJ,Juarez MA,Williams S,Wheeler M,Bean K,Allison TF,Moore HD,Andrews PW,Barbaric I

更新日期：2016-11-08 00:00:00

abstract：:The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with r...

journal_title：Stem cell reports

authors： Naldaiz-Gastesi N,Goicoechea M,Alonso-Martín S,Aiastui A,López-Mayorga M,García-Belda P,Lacalle J,San José C,Araúzo-Bravo MJ,Trouilh L,Anton-Leberre V,Herrero D,Matheu A,Bernad A,García-Verdugo JM,Carvajal JJ,Relaix F,L

更新日期：2016-09-13 00:00:00

abstract：:Mitochondria are critical to neurogenesis, but the mechanisms of mitochondria in neurogenesis have not been well explored. We fully characterized mitochondrial alterations and function in relation to the development of human induced pluripotent stem cell (hiPSC)-derived dopaminergic (DA) neurons. Following directed di...

journal_title：Stem cell reports

authors： Fang D,Qing Y,Yan S,Chen D,Yan SS

更新日期：2016-10-11 00:00:00

abstract：:Human pluripotent stem cell-derived vascular smooth muscle cells (hPSC-VSMCs) are of great value for disease modeling, drug screening, cell therapies, and tissue engineering. However, producing a high quantity of hPSC-VSMCs with current cell culture technologies remains very challenging. Here, we report a scalable met...

journal_title：Stem cell reports

authors： Lin H,Qiu X,Du Q,Li Q,Wang O,Akert L,Wang Z,Anderson D,Liu K,Gu L,Zhang C,Lei Y

更新日期：2019-01-08 00:00:00

abstract：:In placental mammals, balanced expression of X-linked genes is accomplished by X chromosome inactivation (XCI) in female cells. In humans, random XCI is initiated early during embryonic development. To investigate whether reprogramming of female human fibroblasts into induced pluripotent stem cells (iPSCs) leads to re...

journal_title：Stem cell reports

authors： Barakat TS,Ghazvini M,de Hoon B,Li T,Eussen B,Douben H,van der Linden R,van der Stap N,Boter M,Laven JS,Galjaard RJ,Grootegoed JA,de Klein A,Gribnau J

更新日期：2015-02-10 00:00:00

abstract：:Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulat...

journal_title：Stem cell reports

authors： Wang S,Wang X,Ma L,Lin X,Zhang D,Li Z,Wu Y,Zheng C,Feng X,Liao S,Feng Y,Chen J,Hu X,Wang M,Han C

更新日期：2016-07-12 00:00:00

abstract：:The transcription factor (TF) GATA2 plays a key role in organ development and cell fate control in the central nervous, urogenital, respiratory, and reproductive systems, and in primitive and definitive hematopoiesis. Here, we generate a knockin protein reporter mouse line expressing a GATA2VENUS fusion from the endog...

journal_title：Stem cell reports

authors： Ahmed N,Kunz L,Hoppe PS,Loeffler D,Etzrodt M,Ortega GC,Hilsenbeck O,Anastassiadis K,Schroeder T

更新日期：2020-08-11 00:00:00

abstract：:Genetically engineered human pluripotent stem cells (hPSCs) have been proposed as a source for transplantation therapies and are rapidly becoming valuable tools for human disease modeling. However, many applications are limited due to the lack of robust differentiation paradigms that allow for the isolation of defined...

journal_title：Stem cell reports

authors： Forster R,Chiba K,Schaeffer L,Regalado SG,Lai CS,Gao Q,Kiani S,Farin HF,Clevers H,Cost GJ,Chan A,Rebar EJ,Urnov FD,Gregory PD,Pachter L,Jaenisch R,Hockemeyer D

更新日期：2014-06-03 00:00:00

abstract：:Diminished mitochondrial function is causally related to some heart diseases. Here, we developed a human disease model based on cardiomyocytes from human embryonic stem cells (hESCs), in which an important pathway of mitochondrial gene expression was inactivated. Repression of PGC-1α, which is normally induced during ...

journal_title：Stem cell reports

authors： Birket MJ,Casini S,Kosmidis G,Elliott DA,Gerencser AA,Baartscheer A,Schumacher C,Mastroberardino PG,Elefanty AG,Stanley EG,Mummery CL

更新日期：2013-12-12 00:00:00

abstract：:Differentiation of human embryonic stem cells into pancreatic β cells holds great promise for the treatment of diabetes. Recent advances have led to the production of glucose-responsive insulin-secreting cells in vitro, but resulting cells remain less mature than their adult primary β cell counterparts. The barrier(s)...

journal_title：Stem cell reports

authors： Zhou X,Nair GG,Russ HA,Belair CD,Li ML,Shveygert M,Hebrok M,Blelloch R

更新日期：2020-01-14 00:00:00

abstract：:Human induced pluripotent stem cell (hiPSC) culture has become routine, yet the cost of pluripotent cell media, frequent medium changes, and the reproducibility of differentiation have remained restrictive. Here, we describe the formulation of a hiPSC culture medium (B8) as a result of the exhaustive optimization of m...

journal_title：Stem cell reports

authors： Kuo HH,Gao X,DeKeyser JM,Fetterman KA,Pinheiro EA,Weddle CJ,Fonoudi H,Orman MV,Romero-Tejeda M,Jouni M,Blancard M,Magdy T,Epting CL,George AL Jr,Burridge PW

更新日期：2020-02-11 00:00:00

abstract：:Microglia, the immune cells of the brain, are crucial to proper development and maintenance of the CNS, and their involvement in numerous neurological disorders is increasingly being recognized. To improve our understanding of human microglial biology, we devised a chemically defined protocol to generate human microgl...

journal_title：Stem cell reports

authors： Douvaras P,Sun B,Wang M,Kruglikov I,Lallos G,Zimmer M,Terrenoire C,Zhang B,Gandy S,Schadt E,Freytes DO,Noggle S,Fossati V

更新日期：2017-06-06 00:00:00

abstract：:Unambiguous cell line authentication is essential to avoid loss of association between data and cells. The risk for loss of references increases with the rapidity that new human pluripotent stem cell (hPSC) lines are generated, exchanged, and implemented. Ideally, a single name should be used as a generally applied re...

journal_title：Stem cell reports

authors： Kurtz A,Seltmann S,Bairoch A,Bittner MS,Bruce K,Capes-Davis A,Clarke L,Crook JM,Daheron L,Dewender J,Faulconbridge A,Fujibuchi W,Gutteridge A,Hei DJ,Kim YO,Kim JH,Kokocinski AK,Lekschas F,Lomax GP,Loring JF,Ludwig

更新日期：2018-01-09 00:00:00

abstract：:Precise methods for transgene regulation are important to study signaling pathways and cell lineages in biological systems where gene function is often recycled within and across lineages. We engineered a genetic toolset for flexible transgene regulation in these diverse cellular contexts. Specifically, we created an ...

journal_title：Stem cell reports

authors： Akhtar AA,Molina J,Dutra-Clarke M,Kim GB,Levy R,Schreiber-Stainthorp W,Danielpour M,Breunig JJ

更新日期：2015-03-10 00:00:00

abstract：:Mammalian haploid embryonic stem cells (haESCs) hold great promise for functional genetic studies and forward screening. However, all established haploid cells are prone to spontaneous diploidization during long-term culture, rendering application challenging. Here, we report a genome-wide loss-of-function screening t...

journal_title：Stem cell reports

authors： Zhang G,Li X,Sun Y,Wang X,Liu G,Huang Y

更新日期：2021-01-12 00:00:00

abstract：:Emerging evidence suggests that Parkinson's disease (PD), besides being an age-associated disorder, might also have a neurodevelopment component. Disruption of mitochondrial homeostasis has been highlighted as a crucial cofactor in its etiology. Here, we show that PD patient-specific human neuroepithelial stem cells (...

journal_title：Stem cell reports

authors： Walter J,Bolognin S,Antony PMA,Nickels SL,Poovathingal SK,Salamanca L,Magni S,Perfeito R,Hoel F,Qing X,Jarazo J,Arias-Fuenzalida J,Ignac T,Monzel AS,Gonzalez-Cano L,Pereira de Almeida L,Skupin A,Tronstad KJ,Schwamborn

更新日期：2019-05-14 00:00:00

abstract：:Research in human induced pluripotent stem cells (hiPSCs) is rapidly developing and there are expectations that this research may obviate the need to use human embryonic stem cells (hESCs), the ethics of which has been a subject of controversy for more than 15 years. In this study, we investigated approximately 3,400 ...

journal_title：Stem cell reports

authors： Kobold S,Guhr A,Kurtz A,Löser P

更新日期：2015-05-12 00:00:00

abstract：:Teratoma formation is the gold standard assay for testing the capacity of human pluripotent stem cells to differentiate into all embryonic germ layers. Although widely used, little effort has been made to transform this qualitative assay into a quantitative one. Using gene expression data from a wide variety of cells,...

journal_title：Stem cell reports

authors： Avior Y,Biancotti JC,Benvenisty N

更新日期：2015-06-09 00:00:00

abstract：:Hepatic steatosis is a highly prevalent liver disease, yet research is hampered by the lack of tractable cellular and animal models. Steatosis also occurs in cats, where it can cause severe hepatic failure. Previous studies demonstrate the potential of liver organoids for modeling genetic diseases. To examine the poss...

journal_title：Stem cell reports

authors： Kruitwagen HS,Oosterhoff LA,Vernooij IGWH,Schrall IM,van Wolferen ME,Bannink F,Roesch C,van Uden L,Molenaar MR,Helms JB,Grinwis GCM,Verstegen MMA,van der Laan LJW,Huch M,Geijsen N,Vries RG,Clevers H,Rothuizen J,Schota

更新日期：2017-04-11 00:00:00

abstract：:Several studies have reported an association between the farnesoid X receptor alpha (FXRα) and estrogenic signaling pathways. Fxrα could thus be involved in the reprotoxic effects of endocrine disruptors such as bisphenol-A (BPA). To test this hypothesis, mice were exposed to BPA and/or stigmasterol (S), an FXRα antag...

journal_title：Stem cell reports

authors： Sèdes L,Desdoits-Lethimonier C,Rouaisnel B,Holota H,Thirouard L,Lesne L,Damon-Soubeyrand C,Martinot E,Saru JP,Mazaud-Guittot S,Caira F,Beaudoin C,Jégou B,Volle DH

更新日期：2018-10-09 00:00:00