Abstract：
:Human stem cell-derived models of development and neurodegenerative diseases are challenged by cellular immaturity in vitro. Microengineered organ-on-chip (or Organ-Chip) systems are designed to emulate microvolume cytoarchitecture and enable co-culture of distinct cell types. Brain microvascular endothelial cells (BMECs) share common signaling pathways with neurons early in development, but their contribution to human neuronal maturation is largely unknown. To study this interaction and influence of microculture, we derived both spinal motor neurons and BMECs from human induced pluripotent stem cells and observed increased calcium transient function and Chip-specific gene expression in Organ-Chips compared with 96-well plates. Seeding BMECs in the Organ-Chip led to vascular-neural interaction and specific gene activation that further enhanced neuronal function and in vivo-like signatures. The results show that the vascular system has specific maturation effects on spinal cord neural tissue, and the use of Organ-Chips can move stem cell models closer to an in vivo condition.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Sances S,Ho R,Vatine G,West D,Laperle A,Meyer A,Godoy M,Kay PS,Mandefro B,Hatata S,Hinojosa C,Wen N,Sareen D,Hamilton GA,Svendsen CNdoi
10.1016/j.stemcr.2018.02.012subject
Has Abstractpub_date
2018-04-10 00:00:00pages
1222-1236issue
4issn
2213-6711pii
S2213-6711(18)30103-6journal_volume
10pub_type
杂志文章abstract：:A method to maintain and rebuild ureteric bud (UB)-like structures from UB cells in vitro could provide a useful tool for kidney regeneration. We aimed in our present study to establish a serum-free culture system that enables the expansion of UB progenitor cells, i.e., UB tip cells, and reconstruction of UB-like stru...
journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
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journal_title：Stem cell reports
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2016.05.010
更新日期：2016-06-14 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.07.010
更新日期：2020-09-08 00:00:00
abstract：:The recent development of targeted murine reporter alleles as proxies for intestinal stem cell activity has led to significant advances in our understanding of somatic stem cell hierarchies and dynamics. Analysis of these reporters has led to a model in which an indispensable reserve stem cell at the top of the hierar...
journal_title：Stem cell reports
pub_type： 杂志文章
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更新日期：2014-11-11 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.11.002
更新日期：2018-12-11 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.11.016
更新日期：2021-01-12 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
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更新日期：2016-11-08 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.12.019
更新日期：2021-01-20 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2013.04.004
更新日期：2013-06-04 00:00:00
abstract：:Reappraisal of neuropathological studies suggests that pathological hallmarks of Alzheimer's disease and Parkinson's disease (PD) spread progressively along predictable neuronal pathways in the human brain through unknown mechanisms. Although there is much evidence supporting the prion-like propagation and amplificati...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.12.007
更新日期：2019-02-12 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2015.07.014
更新日期：2015-09-08 00:00:00
abstract：:In placental mammals, balanced expression of X-linked genes is accomplished by X chromosome inactivation (XCI) in female cells. In humans, random XCI is initiated early during embryonic development. To investigate whether reprogramming of female human fibroblasts into induced pluripotent stem cells (iPSCs) leads to re...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2014.12.012
更新日期：2015-02-10 00:00:00
abstract：:Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput ima...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2017.08.023
更新日期：2017-10-10 00:00:00
abstract：:Thick-filament sarcomere mutations are a common cause of hypertrophic cardiomyopathy (HCM), a disorder of heart muscle thickening associated with sudden cardiac death and heart failure, with unclear mechanisms. We engineered four isogenic induced pluripotent stem cell (iPSC) models of β-myosin heavy chain and myosin-b...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2018.11.015
更新日期：2019-01-08 00:00:00
abstract：:Teratoma formation is the gold standard assay for testing the capacity of human pluripotent stem cells to differentiate into all embryonic germ layers. Although widely used, little effort has been made to transform this qualitative assay into a quantitative one. Using gene expression data from a wide variety of cells,...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2015.05.006
更新日期：2015-06-09 00:00:00
abstract：:The in vitro derivation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) is complicated by the existence of multiple overlapping embryonic blood cell programs called primitive, erythromyeloid progenitor (EMP), and definitive. As HSCs are only generated during the definitive stage of hematopoiesis,...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2015.07.008
更新日期：2015-08-11 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
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更新日期：2013-09-26 00:00:00
abstract：:ADP-ribosylation factor-like 4aa (Arl4aa) is a member of the ADP-ribosylation factor family. It is expressed in hematopoietic tissue during embryonic development, but its function was unknown. Zebrafish arl4aa is preferentially expressed in the ventral wall of the dorsal aorta (VDA) at 24 and 36 hpf and in caudal hema...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.02.012
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abstract：:Pluripotent stem cells have been proposed as an unlimited source of pancreatic β cells for studying and treating diabetes. However, the long, multi-step differentiation protocols used to generate functional β cells inevitably exhibit considerable variability, particularly when applied to pluripotent cells from diverse...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2017.05.019
更新日期：2017-06-06 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2015.05.007
更新日期：2015-07-14 00:00:00
abstract：:Microglia have recently been established as key regulators of brain development. However, their role in neuronal subtype specification remains largely unknown. Using three different co-culture setups, we show that microglia-secreted factors enhance dopaminergic differentiation of somatic and induced pluripotent stem c...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.12.011
更新日期：2021-01-06 00:00:00
abstract：:Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulat...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2016.05.013
更新日期：2016-07-12 00:00:00
abstract：:Spermatogonial stem cells (SSCs) serve as a resource for producing genetically modified animals. However, genetic manipulation of SSCs has met with limited success. Here, we show efficient gene transfer into SSCs via a lentivirus (FV-LV) using a fusion protein (F), a Sendai virus (SV) envelope protein involved in viri...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.02.001
更新日期：2020-03-10 00:00:00
abstract：:Human embryonic stem cells (hESCs) provide a platform for studying human development and understanding mechanisms underlying diseases. Retinoblastoma-1 (RB1) is a key regulator of cell cycling, of which biallelic inactivation initiates retinoblastoma, the most common congenital intraocular malignancy. We developed a m...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2017.03.005
更新日期：2017-05-09 00:00:00
abstract：:Hematopoietic stem cells (HSCs) exist in a dormant state and progressively lose regenerative potency as they undergo successive divisions. Why this functional decline occurs and how this information is encoded is unclear. To better understand how this information is stored, we performed RNA sequencing on HSC populatio...
journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2020.03.005
更新日期：2020-04-14 00:00:00
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journal_title：Stem cell reports
pub_type： 杂志文章
doi：10.1016/j.stemcr.2017.08.025
更新日期：2017-11-14 00:00:00
abstract：:The environment surrounding stem cells has the ability to elicit profound, heritable epigenetic changes orchestrated by multiple epigenetic mechanisms, which can be modulated by the level of specific metabolites. In this review, we highlight the significance of metabolism in regulating stem cell homeostasis, cell stat...
journal_title：Stem cell reports
pub_type： 杂志文章,评审
doi：10.1016/j.stemcr.2019.09.003
更新日期：2019-10-08 00:00:00
