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In Vitro Differentiated Human Stem Cell-Derived Neurons Reproduce Synaptic Synchronicity Arising during Neurodevelopment.

Abstract：

:Neurons differentiated from induced pluripotent stem cells (iPSCs) typically show regular spiking and synaptic activity but lack more complex network activity critical for brain development, such as periodic depolarizations including simultaneous involvement of glutamatergic and GABAergic neurotransmission. We generated human iPSC-derived neurons exhibiting spontaneous oscillatory activity after cultivation of up to 6 months, which resembles early oscillations observed in rodent neurons. This behavior was found in neurons generated using a more "native" embryoid body protocol, in contrast to a "fast" protocol based on NGN2 overexpression. A comparison with published data indicates that EB-derived neurons reach the maturity of neurons of the third trimester and NGN2-derived neurons of the second trimester of human gestation. Co-culturing NGN2-derived neurons with astrocytes only led to a partial compensation and did not reliably induce complex network activity. Our data will help selection of the appropriate iPSC differentiation assay to address specific questions related to neurodevelopmental disorders.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Rosa F,Dhingra A,Uysal B,Mendis GDC,Loeffler H,Elsen G,Mueller S,Schwarz N,Castillo-Lizardo M,Cuddy C,Becker F,Heutink P,Reid CA,Petrou S,Lerche H,Maljevic S

doi

10.1016/j.stemcr.2020.05.015

subject

Has Abstract

pub_date

2020-07-14 00:00:00

pages

22-37

issue

1

issn

2213-6711

pii

S2213-6711(20)30187-9

journal_volume

15

pub_type

杂志文章

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