Abstract:
:We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450-500 nm) is readily observed by fluorescence microscopy and correlates with the expression of pluripotency markers (OCT4, SOX2, and NANOG). It allows easy identification and isolation of undifferentiated human pluripotent stem cells, high-throughput fluorescence sorting and subsequent propagation. The fluorescence appears early during somatic reprogramming. We show that the blue fluorescence arises from the sequestration of retinyl esters in cytoplasmic lipid bodies. The retinoid-sequestering lipid bodies are specific to human and mouse pluripotent stem cells of the primed or epiblast-like state and absent in naive mouse embryonic stem cells. Retinol, present in widely used stem cell culture media, is sequestered as retinyl ester specifically by primed pluripotent cells and also can induce the formation of these lipid bodies.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Muthusamy T,Mukherjee O,Menon R,Megha PB,Panicker MMdoi
10.1016/j.stemcr.2014.05.004subject
Has Abstractpub_date
2014-06-12 00:00:00pages
169-84issue
1issn
2213-6711pii
S2213-6711(14)00145-3journal_volume
3pub_type
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