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Engineered Microenvironment for Manufacturing Human Pluripotent Stem Cell-Derived Vascular Smooth Muscle Cells.

Abstract：

:Human pluripotent stem cell-derived vascular smooth muscle cells (hPSC-VSMCs) are of great value for disease modeling, drug screening, cell therapies, and tissue engineering. However, producing a high quantity of hPSC-VSMCs with current cell culture technologies remains very challenging. Here, we report a scalable method for manufacturing hPSC-VSMCs in alginate hydrogel microtubes (i.e., AlgTubes), which protect cells from hydrodynamic stresses and limit cell mass to <400 μm to ensure efficient mass transport. The tubes provide cells a friendly microenvironment, leading to extremely high culture efficiency. We have shown that hPSC-VSMCs can be generated in 10 days with high viability, high purity, and high yield (∼5.0 × 108 cells/mL). Phenotype and gene expression showed that VSMCs made in AlgTubes and VSMCs made in 2D cultures were similar overall. However, AlgTube-VSMCs had higher expression of genes related to vasculature development and angiogenesis, and 2D-VSMCs had higher expression of genes related to cell death and biosynthetic processes.

journal_name

Stem Cell Reports

journal_title

Stem cell reports

authors

Lin H,Qiu X,Du Q,Li Q,Wang O,Akert L,Wang Z,Anderson D,Liu K,Gu L,Zhang C,Lei Y

doi

10.1016/j.stemcr.2018.11.009

subject

Has Abstract

pub_date

2019-01-08 00:00:00

pages

84-97

issue

1

issn

2213-6711

pii

S2213-6711(18)30477-6

journal_volume

12

pub_type

杂志文章

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