Abstract:
:Cell division is a process by which a mother cell divides into genetically identical sister cells, although sister cells often display considerable diversity. In this report, over 350 sister embryonic stem cells (ESCs) were isolated through a microdissection method, and then expression levels of 48 key genes were examined for each sister cell. Our system revealed considerable diversities between sister ESCs at both pluripotent and differentiated states, whereas the similarity between sister ESCs was significantly elevated in a 2i (MEK and GSK3b inhibitors) condition, which is believed to mimic the ground state of pluripotency. DNA methyltransferase 3a/3b were downregulated in 2i-grown ESCs, and the loss of DNA methyltransferases was sufficient to generate nearly identical sister cells. These results suggest that DNA methylation is a major cause of the diversity between sister cells at the pluripotent states, and thus demethylation per se plays an important role in promoting ESC's self-renewal.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Jasnos L,Aksoy FB,Hersi HM,Wantuch S,Sawado Tdoi
10.1016/j.stemcr.2013.08.005subject
Has Abstractpub_date
2013-09-26 00:00:00pages
360-9issue
4issn
2213-6711pii
S2213-6711(13)00071-4journal_volume
1pub_type
杂志文章abstract::The International Society for Stem Cell Research 11(th) Annual Meeting was held in Boston in June 2013, bringing together just over 4000 attendees. An emphasis on therapeutic applications in many talks reflected the maturation of the stem cell field from its origins in basic science to one that is beginning to show th...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2013.12.004
更新日期:2013-01-01 00:00:00
abstract::Both glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells, whereas retinoic acid (RA) induces spermatogonial differentiation. In this study, we investigated the functional differences between FGF2 and GDNF in the germl...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.03.016
更新日期:2018-06-05 00:00:00
abstract::FAM122A is a highly conserved housekeeping gene, but its physiological and pathophysiological roles remain greatly elusive. Based on the fact that FAM122A is highly expressed in human CD71+ early erythroid cells, herein we report that FAM122A is downregulated during erythroid differentiation, while its overexpression ...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2020.07.010
更新日期:2020-09-08 00:00:00
abstract::Fanconi anemia (FA) causes bone marrow failure early during childhood, and recent studies indicate that a hematopoietic defect could begin in utero. We performed a unique kinetics study of hematopoiesis in Fancg-/- mouse embryos, between the early embryonic day 11.5 (E11.5) to E12.5 developmental window (when the high...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.10.001
更新日期:2018-11-13 00:00:00
abstract::Direct reprogramming of adult somatic cells into alternative cell types has been shown for several lineages. We previously showed that GATA4, MEF2C, and TBX5 (GMT) directly reprogrammed nonmyocyte mouse heart cells into induced cardiomyocyte-like cells (iCMs) in vitro and in vivo. However, GMT alone appears insufficie...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2013.07.005
更新日期:2013-08-22 00:00:00
abstract::The balance of self-renewal and differentiation in long-term repopulating hematopoietic stem cells (LT-HSC) must be strictly controlled to maintain blood homeostasis and to prevent leukemogenesis. Hematopoietic cytokines can induce differentiation in LT-HSCs; however, the molecular mechanism orchestrating this delicat...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2014.05.010
更新日期:2014-06-19 00:00:00
abstract::Mitochondria are critical to neurogenesis, but the mechanisms of mitochondria in neurogenesis have not been well explored. We fully characterized mitochondrial alterations and function in relation to the development of human induced pluripotent stem cell (hiPSC)-derived dopaminergic (DA) neurons. Following directed di...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.08.014
更新日期:2016-10-11 00:00:00
abstract::In addition to their promise in regenerative medicine, pluripotent stem cells have proved to be faithful models of many human diseases. In particular, patient-specific stem cell-derived cardiomyocytes recapitulate key features of several life-threatening cardiac arrhythmia syndromes. For both modeling and regenerative...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2014.01.003
更新日期:2014-02-06 00:00:00
abstract::Tight regulation of the balance between self-renewal and differentiation of neural stem cells is crucial to assure proper neural development. In this context, Notch signaling is a well-known promoter of stemness. In contrast, the bifunctional brain-enriched microRNA miR-9/9(∗) has been implicated in promoting neuronal...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.06.008
更新日期:2016-08-09 00:00:00
abstract::Human stem cell-derived models of development and neurodegenerative diseases are challenged by cellular immaturity in vitro. Microengineered organ-on-chip (or Organ-Chip) systems are designed to emulate microvolume cytoarchitecture and enable co-culture of distinct cell types. Brain microvascular endothelial cells (BM...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.02.012
更新日期:2018-04-10 00:00:00
abstract::Physical activity is a strong positive physiological modulator of adult neurogenesis in the hippocampal dentate gyrus. Although the underlying regulatory mechanisms are still unknown, systemic processes must be involved. Here we show that platelets are activated after acute periods of running, and that activated plate...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2019.02.009
更新日期:2019-04-09 00:00:00
abstract::Stem Cell Reports frequently receives manuscripts dealing with the topic of cancer stem cells. Many of the submissions on this topic have major shortcomings in their content or limits to the conclusions that can be drawn from the results presented. The purpose of this Commentary is to highlight some of the underlying ...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2020.01.011
更新日期:2020-02-11 00:00:00
abstract::A method to maintain and rebuild ureteric bud (UB)-like structures from UB cells in vitro could provide a useful tool for kidney regeneration. We aimed in our present study to establish a serum-free culture system that enables the expansion of UB progenitor cells, i.e., UB tip cells, and reconstruction of UB-like stru...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.12.011
更新日期:2017-02-14 00:00:00
abstract::Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2017.10.003
更新日期:2017-11-14 00:00:00
abstract::We describe the use of a characteristic blue fluorescence to identify and isolate pluripotent human embryonic stem cells and human-induced pluripotent stem cells. The blue fluorescence emission (450-500 nm) is readily observed by fluorescence microscopy and correlates with the expression of pluripotency markers (OCT4,...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2014.05.004
更新日期:2014-06-12 00:00:00
abstract::We established two human embryonic stem cell (hESC) lines with a GGGGCC expansion in the C9orf72 gene (C9), and compared them with haploidentical and unrelated C9 induced pluripotent stem cells (iPSCs). We found a marked difference in C9 methylation between the cells. hESCs and parental fibroblasts are entirely unmeth...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.09.011
更新日期:2016-11-08 00:00:00
abstract::The recent development of targeted murine reporter alleles as proxies for intestinal stem cell activity has led to significant advances in our understanding of somatic stem cell hierarchies and dynamics. Analysis of these reporters has led to a model in which an indispensable reserve stem cell at the top of the hierar...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2014.09.011
更新日期:2014-11-11 00:00:00
abstract::Differentiation of human embryonic stem cells into pancreatic β cells holds great promise for the treatment of diabetes. Recent advances have led to the production of glucose-responsive insulin-secreting cells in vitro, but resulting cells remain less mature than their adult primary β cell counterparts. The barrier(s)...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2019.11.009
更新日期:2020-01-14 00:00:00
abstract::Thick-filament sarcomere mutations are a common cause of hypertrophic cardiomyopathy (HCM), a disorder of heart muscle thickening associated with sudden cardiac death and heart failure, with unclear mechanisms. We engineered four isogenic induced pluripotent stem cell (iPSC) models of β-myosin heavy chain and myosin-b...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.11.015
更新日期:2019-01-08 00:00:00
abstract::Mammalian haploid embryonic stem cells (haESCs) hold great promise for functional genetic studies and forward screening. However, all established haploid cells are prone to spontaneous diploidization during long-term culture, rendering application challenging. Here, we report a genome-wide loss-of-function screening t...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2020.11.016
更新日期:2021-01-12 00:00:00
abstract::Induced pluripotent stem cells were created from a pancreas agenesis patient with a mutation in GATA6. Using genome-editing technology, additional stem cell lines with mutations in both GATA6 alleles were generated and demonstrated a severe block in definitive endoderm induction, which could be rescued by re-expressio...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.12.026
更新日期:2017-03-14 00:00:00
abstract::Human stem cell models have the potential to provide platforms for phenotypic screens to identify candidate treatments and cellular pathways involved in the pathogenesis of neurodegenerative disorders. Amyloid precursor protein (APP) processing and the accumulation of APP-derived amyloid β (Aβ) peptides are key proces...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2017.02.006
更新日期:2017-04-11 00:00:00
abstract::Although pluripotent stem cells can generate various types of differentiated cells, it is unclear why lineage-committed stem/progenitor cells derived from pluripotent stem cells are decelerated and why the differentiation-resistant propensity of embryonic stem cell (ESC)/induced pluripotent stem cell (iPSC)-derived ce...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.12.018
更新日期:2019-02-12 00:00:00
abstract::There are currently hundreds of businesses across the United States offering direct-to-consumer stem cell treatments that have not been through regulatory approval by the Food and Drug Administration (FDA). Here, we provide a detailed characterization of nearly 170 stem cell businesses operating in the Southwest Unite...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2019.07.001
更新日期:2019-08-13 00:00:00
abstract::Reprogramming of somatic cells produces induced pluripotent stem cells (iPSCs) that are invaluable resources for biomedical research. Here, we extended the previous transcriptome studies by performing RNA-seq on cells defined by a combination of multiple cellular surface markers. We found that transcriptome changes du...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2015.04.009
更新日期:2015-06-09 00:00:00
abstract::Understanding the cellular properties controlling neural stem and progenitor cell (NSPC) fate choice will improve their therapeutic potential. The electrophysiological measure whole-cell membrane capacitance reflects fate bias in the neural lineage but the cellular properties underlying membrane capacitance are poorly...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2018.08.011
更新日期:2018-10-09 00:00:00
abstract::Spermatogonial stem cells (SSCs) serve as a resource for producing genetically modified animals. However, genetic manipulation of SSCs has met with limited success. Here, we show efficient gene transfer into SSCs via a lentivirus (FV-LV) using a fusion protein (F), a Sendai virus (SV) envelope protein involved in viri...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2020.02.001
更新日期:2020-03-10 00:00:00
abstract::The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with r...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2016.08.002
更新日期:2016-09-13 00:00:00
abstract::Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2015.01.020
更新日期:2015-03-10 00:00:00
abstract::Cardiomyocytes derived from human pluripotent stem cells (hPSCs) are a promising cell source for regenerative medicine, disease modeling, and drug discovery, all of which require enriched cardiomyocytes, ideally ones with mature phenotypes. However, current methods are typically performed in 2D environments that produ...
journal_title:Stem cell reports
pub_type: 杂志文章
doi:10.1016/j.stemcr.2014.06.002
更新日期:2014-08-12 00:00:00