Abstract:
:We established two human embryonic stem cell (hESC) lines with a GGGGCC expansion in the C9orf72 gene (C9), and compared them with haploidentical and unrelated C9 induced pluripotent stem cells (iPSCs). We found a marked difference in C9 methylation between the cells. hESCs and parental fibroblasts are entirely unmethylated while the iPSCs are hypermethylated. In addition, we show that the expansion alters promoter usage and interferes with the proper splicing of intron 1, eventually leading to the accumulation of repeat-containing mRNA following neural differentiation. These changes are attenuated in C9 iPSCs, presumably owing to hypermethylation. Altogether, this study highlights the importance of neural differentiation in the pathogenesis of disease and points to the potential role of hypermethylation as a neuroprotective mechanism against pathogenic mRNAs, envisaging a milder phenotype in C9 iPSCs.
journal_name
Stem Cell Reportsjournal_title
Stem cell reportsauthors
Cohen-Hadad Y,Altarescu G,Eldar-Geva T,Levi-Lahad E,Zhang M,Rogaeva E,Gotkine M,Bartok O,Ashwal-Fluss R,Kadener S,Epsztejn-Litman S,Eiges Rdoi
10.1016/j.stemcr.2016.09.011subject
Has Abstractpub_date
2016-11-08 00:00:00pages
927-940issue
5issn
2213-6711pii
S2213-6711(16)30217-Xjournal_volume
7pub_type
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