Cancer Stem Cells: Notes for Authors.


:Stem Cell Reports frequently receives manuscripts dealing with the topic of cancer stem cells. Many of the submissions on this topic have major shortcomings in their content or limits to the conclusions that can be drawn from the results presented. The purpose of this Commentary is to highlight some of the underlying issues so that authors can enhance the strength of their research contributions.


Stem Cell Reports


Stem cell reports


Eaves CJ,Pera MF




Has Abstract


2020-02-11 00:00:00












  • Prospective Isolation and Comparison of Human Germinal Matrix and Glioblastoma EGFR+ Populations with Stem Cell Properties.

    abstract::Characterization of non-neoplastic and malignant human stem cell populations in their native state can provide new insights into gliomagenesis. Here we developed a purification strategy to directly isolate EGFR+/- populations from human germinal matrix (GM) and adult subventricular zone autopsy tissues, and from de no...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Tome-Garcia J,Tejero R,Nudelman G,Yong RL,Sebra R,Wang H,Fowkes M,Magid M,Walsh M,Silva-Vargas V,Zaslavsky E,Friedel RH,Doetsch F,Tsankova NM

    更新日期:2017-05-09 00:00:00

  • Targeted Repair of p47-CGD in iPSCs by CRISPR/Cas9: Functional Correction without Cleavage in the Highly Homologous Pseudogenes.

    abstract::Mutations in the NADPH oxidase, which is crucial for the respiratory burst in phagocytes, result in chronic granulomatous disease (CGD). The only curative treatment option for CGD patients, who suffer from severe infections, is allogeneic bone marrow transplantation. Over 90% of patients with mutations in the p47phox ...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Klatt D,Cheng E,Philipp F,Selich A,Dahlke J,Schmidt RE,Schott JW,Büning H,Hoffmann D,Thrasher AJ,Schambach A

    更新日期:2019-10-08 00:00:00

  • Propagation of α-Synuclein Strains within Human Reconstructed Neuronal Network.

    abstract::Reappraisal of neuropathological studies suggests that pathological hallmarks of Alzheimer's disease and Parkinson's disease (PD) spread progressively along predictable neuronal pathways in the human brain through unknown mechanisms. Although there is much evidence supporting the prion-like propagation and amplificati...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Gribaudo S,Tixador P,Bousset L,Fenyi A,Lino P,Melki R,Peyrin JM,Perrier AL

    更新日期:2019-02-12 00:00:00

  • Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture.

    abstract::Human induced pluripotent stem cell (hiPSC) culture has become routine, yet the cost of pluripotent cell media, frequent medium changes, and the reproducibility of differentiation have remained restrictive. Here, we describe the formulation of a hiPSC culture medium (B8) as a result of the exhaustive optimization of m...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Kuo HH,Gao X,DeKeyser JM,Fetterman KA,Pinheiro EA,Weddle CJ,Fonoudi H,Orman MV,Romero-Tejeda M,Jouni M,Blancard M,Magdy T,Epting CL,George AL Jr,Burridge PW

    更新日期:2020-02-11 00:00:00

  • Enhanced Reconstitution of Human Erythropoiesis and Thrombopoiesis in an Immunodeficient Mouse Model with Kit(Wv) Mutations.

    abstract::In human-to-mouse xenograft models, reconstitution of human hematopoiesis is usually B-lymphoid dominant. Here we show that the introduction of homozygous Kit(Wv) mutations into C57BL/6.Rag2(null)Il2rg(null) mice with NOD-Sirpa (BRGS) strongly promoted human multi-lineage reconstitution. After xenotransplantation of h...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Yurino A,Takenaka K,Yamauchi T,Nunomura T,Uehara Y,Jinnouchi F,Miyawaki K,Kikushige Y,Kato K,Miyamoto T,Iwasaki H,Kunisaki Y,Akashi K

    更新日期:2016-09-13 00:00:00

  • GATA6 Plays an Important Role in the Induction of Human Definitive Endoderm, Development of the Pancreas, and Functionality of Pancreatic β Cells.

    abstract::Induced pluripotent stem cells were created from a pancreas agenesis patient with a mutation in GATA6. Using genome-editing technology, additional stem cell lines with mutations in both GATA6 alleles were generated and demonstrated a severe block in definitive endoderm induction, which could be rescued by re-expressio...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Tiyaboonchai A,Cardenas-Diaz FL,Ying L,Maguire JA,Sim X,Jobaliya C,Gagne AL,Kishore S,Stanescu DE,Hughes N,De Leon DD,French DL,Gadue P

    更新日期:2017-03-14 00:00:00

  • Induced Developmental Arrest of Early Hematopoietic Progenitors Leads to the Generation of Leukocyte Stem Cells.

    abstract::Self-renewal potential and multipotency are hallmarks of a stem cell. It is generally accepted that acquisition of such stemness requires rejuvenation of somatic cells through reprogramming of their genetic and epigenetic status.We show here that a simple block of cell differentiation is sufficient to induce and maint...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Ikawa T,Masuda K,Huijskens MJAJ,Satoh R,Kakugawa K,Agata Y,Miyai T,Germeraad WTV,Katsura Y,Kawamoto H

    更新日期:2015-11-10 00:00:00

  • LNGFR(+)THY-1(+)VCAM-1(hi+) cells reveal functionally distinct subpopulations in mesenchymal stem cells.

    abstract::Human mesenchymal stem cells (hMSCs), which conventionally are isolated based on their adherence to plastic, are heterogeneous and have poor growth and differentiation, limiting our ability to investigate their intrinsic characteristics. We report an improved prospective clonal isolation technique and reveal that the ...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Mabuchi Y,Morikawa S,Harada S,Niibe K,Suzuki S,Renault-Mihara F,Houlihan DD,Akazawa C,Okano H,Matsuzaki Y

    更新日期:2013-07-11 00:00:00

  • Identifying division symmetry of mouse embryonic stem cells: negative impact of DNA methyltransferases on symmetric self-renewal.

    abstract::Cell division is a process by which a mother cell divides into genetically identical sister cells, although sister cells often display considerable diversity. In this report, over 350 sister embryonic stem cells (ESCs) were isolated through a microdissection method, and then expression levels of 48 key genes were exam...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Jasnos L,Aksoy FB,Hersi HM,Wantuch S,Sawado T

    更新日期:2013-09-26 00:00:00

  • Sox100B Regulates Progenitor-Specific Gene Expression and Cell Differentiation in the Adult Drosophila Intestine.

    abstract::Robust production of terminally differentiated cells from self-renewing resident stem cells is essential to maintain proper tissue architecture and physiological functions, especially in high-turnover tissues. However, the transcriptional networks that precisely regulate cell transition and differentiation are poorly ...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Meng FW,Rojas Villa SE,Biteau B

    更新日期:2020-02-11 00:00:00

  • Cytokine-regulated GADD45G induces differentiation and lineage selection in hematopoietic stem cells.

    abstract::The balance of self-renewal and differentiation in long-term repopulating hematopoietic stem cells (LT-HSC) must be strictly controlled to maintain blood homeostasis and to prevent leukemogenesis. Hematopoietic cytokines can induce differentiation in LT-HSCs; however, the molecular mechanism orchestrating this delicat...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Thalheimer FB,Wingert S,De Giacomo P,Haetscher N,Rehage M,Brill B,Theis FJ,Hennighausen L,Schroeder T,Rieger MA

    更新日期:2014-06-19 00:00:00

  • Human iPSC-Derived Endothelial Cells and Microengineered Organ-Chip Enhance Neuronal Development.

    abstract::Human stem cell-derived models of development and neurodegenerative diseases are challenged by cellular immaturity in vitro. Microengineered organ-on-chip (or Organ-Chip) systems are designed to emulate microvolume cytoarchitecture and enable co-culture of distinct cell types. Brain microvascular endothelial cells (BM...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Sances S,Ho R,Vatine G,West D,Laperle A,Meyer A,Godoy M,Kay PS,Mandefro B,Hatata S,Hinojosa C,Wen N,Sareen D,Hamilton GA,Svendsen CN

    更新日期:2018-04-10 00:00:00

  • An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs.

    abstract::Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput ima...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Larribère L,Galach M,Novak D,Arévalo K,Volz HC,Stark HJ,Boukamp P,Boutros M,Utikal J

    更新日期:2017-10-10 00:00:00

  • Transcriptional Profiling of Xenogeneic Transplants: Examining Human Pluripotent Stem Cell-Derived Grafts in the Rodent Brain.

    abstract::Human pluripotent stem cells are a valuable resource for transplantation, yet our ability to profile xenografts is largely limited to low-throughput immunohistochemical analysis by difficulties in readily isolating grafts for transcriptomic and/or proteomic profiling. Here, we present a simple methodology utilizing di...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Bye CR,Penna V,de Luzy IR,Gantner CW,Hunt CPJ,Thompson LH,Parish CL

    更新日期:2019-11-12 00:00:00

  • Enhanced Energetic State and Protection from Oxidative Stress in Human Myoblasts Overexpressing BMI1.

    abstract::The Polycomb group gene BMI1 is essential for efficient muscle regeneration in a mouse model of Duchenne muscular dystrophy, and its enhanced expression in adult skeletal muscle satellite cells ameliorates the muscle strength in this model. Here, we show that the impact of mild BMI1 overexpression observed in mouse mo...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Dibenedetto S,Niklison-Chirou M,Cabrera CP,Ellis M,Robson LG,Knopp P,Tedesco FS,Ragazzi M,Di Foggia V,Barnes MR,Radunovic A,Marino S

    更新日期:2017-08-08 00:00:00

  • Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid.

    abstract::In this study we dissected retinal organoid morphogenesis in human embryonic stem cell (hESC)-derived cultures and established a convenient method for isolating large quantities of retinal organoids for modeling human retinal development and disease. Epithelialized cysts were generated via floating culture of clumps o...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Lowe A,Harris R,Bhansali P,Cvekl A,Liu W

    更新日期:2016-05-10 00:00:00

  • Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury.

    abstract::Neural progenitor cells (NPCs) transplanted into sites of spinal cord injury (SCI) extend large numbers of axons into the caudal host spinal cord. We determined the precise locations of neurons in the graft that extend axons into the caudal host spinal cord using AAV9-Cre-initiated retrograde tracing into floxed-TdTom...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Lu P,Gomes-Leal W,Anil S,Dobkins G,Huie JR,Ferguson AR,Graham L,Tuszynski M

    更新日期:2019-07-09 00:00:00

  • Maternal inflammation contributes to brain overgrowth and autism-associated behaviors through altered redox signaling in stem and progenitor cells.

    abstract::A period of mild brain overgrowth with an unknown etiology has been identified as one of the most common phenotypes in autism. Here, we test the hypothesis that maternal inflammation during critical periods of embryonic development can cause brain overgrowth and autism-associated behaviors as a result of altered neura...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Le Belle JE,Sperry J,Ngo A,Ghochani Y,Laks DR,López-Aranda M,Silva AJ,Kornblum HI

    更新日期:2014-11-11 00:00:00

  • Modeling Human Bile Acid Transport and Synthesis in Stem Cell-Derived Hepatocytes with a Patient-Specific Mutation.

    abstract::The bile salt export pump (BSEP) is responsible for the export of bile acid from hepatocytes. Impaired transcellular transport of bile acids in hepatocytes with mutations in BSEP causes cholestasis. Compensatory mechanisms to regulate the intracellular bile acid concentration in human hepatocytes with BSEP deficiency ...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Hayashi H,Osaka S,Sakabe K,Fukami A,Kishimoto E,Aihara E,Sabu Y,Mizutani A,Kusuhara H,Naritaka N,Zhang W,Huppert SS,Sakabe M,Nakamura T,Hu YC,Mayhew C,Setchell K,Takebe T,Asai A

    更新日期:2020-12-30 00:00:00

  • Craniometaphyseal Dysplasia Mutations in ANKH Negatively Affect Human Induced Pluripotent Stem Cell Differentiation into Osteoclasts.

    abstract::We identified osteoclast defects in craniometaphyseal dysplasia (CMD) using an easy-to-use protocol for differentiating osteoclasts from human induced pluripotent stem cells (hiPSCs). CMD is a rare genetic bone disorder, characterized by life-long progressive thickening of craniofacial bones and abnormal shape of long...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Chen IP,Luxmi R,Kanaujiya J,Hao Z,Reichenberger EJ

    更新日期:2017-11-14 00:00:00

  • Isolation of Human Colon Stem Cells Using Surface Expression of PTK7.

    abstract::Insertion of reporter cassettes into the Lgr5 locus has enabled the characterization of mouse intestinal stem cells (ISCs). However, low cell surface abundance of LGR5 protein and lack of high-affinity anti-LGR5 antibodies represent a roadblock to efficiently isolate human colonic stem cells (hCoSCs). We set out to id...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Jung P,Sommer C,Barriga FM,Buczacki SJ,Hernando-Momblona X,Sevillano M,Duran-Frigola M,Aloy P,Selbach M,Winton DJ,Batlle E

    更新日期:2015-12-08 00:00:00

  • Characterizing Direct-to-Consumer Stem Cell Businesses in the Southwest United States.

    abstract::There are currently hundreds of businesses across the United States offering direct-to-consumer stem cell treatments that have not been through regulatory approval by the Food and Drug Administration (FDA). Here, we provide a detailed characterization of nearly 170 stem cell businesses operating in the Southwest Unite...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Frow EK,Brafman DA,Muldoon A,Krum L,Williams P,Becker B,Nelson JP,Pritchett A

    更新日期:2019-08-13 00:00:00

  • Histone H3 Lysine 9 Acetylation Obstructs ATM Activation and Promotes Ionizing Radiation Sensitivity in Normal Stem Cells.

    abstract::Dynamic spatiotemporal modification of chromatin around DNA damage is vital for efficient DNA repair. Normal stem cells exhibit an attenuated DNA damage response (DDR), inefficient DNA repair, and high radiosensitivity. The impact of unique chromatin characteristics of stem cells in DDR regulation is not yet recognize...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Meyer B,Fabbrizi MR,Raj S,Zobel CL,Hallahan DE,Sharma GG

    更新日期:2016-12-13 00:00:00

  • ER Stress and Autophagic Perturbations Lead to Elevated Extracellular α-Synuclein in GBA-N370S Parkinson's iPSC-Derived Dopamine Neurons.

    abstract::Heterozygous mutations in the glucocerebrosidase gene (GBA) represent the strongest common genetic risk factor for Parkinson's disease (PD), the second most common neurodegenerative disorder. However, the molecular mechanisms underlying this association are still poorly understood. Here, we have analyzed ten independe...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Fernandes HJ,Hartfield EM,Christian HC,Emmanoulidou E,Zheng Y,Booth H,Bogetofte H,Lang C,Ryan BJ,Sardi SP,Badger J,Vowles J,Evetts S,Tofaris GK,Vekrellis K,Talbot K,Hu MT,James W,Cowley SA,Wade-Martins R

    更新日期:2016-03-08 00:00:00

  • The Periostin/Integrin-αv Axis Regulates the Size of Hematopoietic Stem Cell Pool in the Fetal Liver.

    abstract::We earlier showed that outside-in integrin signaling through POSTN-ITGAV interaction plays an important role in regulating adult hematopoietic stem cell (HSC) quiescence. Here, we show that Itgav deletion results in increased frequency of phenotypic HSCs in fetal liver (FL) due to faster proliferation. Systemic deleti...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Biswas A,Roy IM,Babu PC,Manesia J,Schouteden S,Vijayakurup V,Anto RJ,Huelsken J,Lacy-Hulbert A,Verfaillie CM,Khurana S

    更新日期:2020-08-11 00:00:00

  • In Vitro Propagation and Branching Morphogenesis from Single Ureteric Bud Cells.

    abstract::A method to maintain and rebuild ureteric bud (UB)-like structures from UB cells in vitro could provide a useful tool for kidney regeneration. We aimed in our present study to establish a serum-free culture system that enables the expansion of UB progenitor cells, i.e., UB tip cells, and reconstruction of UB-like stru...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Yuri S,Nishikawa M,Yanagawa N,Jo OD,Yanagawa N

    更新日期:2017-02-14 00:00:00

  • Retinoic Acid Is Sufficient for the In Vitro Induction of Mouse Spermatocytes.

    abstract::Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulat...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Wang S,Wang X,Ma L,Lin X,Zhang D,Li Z,Wu Y,Zheng C,Feng X,Liao S,Feng Y,Chen J,Hu X,Wang M,Han C

    更新日期:2016-07-12 00:00:00

  • Microscale generation of cardiospheres promotes robust enrichment of cardiomyocytes derived from human pluripotent stem cells.

    abstract::Cardiomyocytes derived from human pluripotent stem cells (hPSCs) are a promising cell source for regenerative medicine, disease modeling, and drug discovery, all of which require enriched cardiomyocytes, ideally ones with mature phenotypes. However, current methods are typically performed in 2D environments that produ...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Nguyen DC,Hookway TA,Wu Q,Jha R,Preininger MK,Chen X,Easley CA,Spearman P,Deshpande SR,Maher K,Wagner MB,McDevitt TC,Xu C

    更新日期:2014-08-12 00:00:00

  • Development and Dynamic Regulation of Mitochondrial Network in Human Midbrain Dopaminergic Neurons Differentiated from iPSCs.

    abstract::Mitochondria are critical to neurogenesis, but the mechanisms of mitochondria in neurogenesis have not been well explored. We fully characterized mitochondrial alterations and function in relation to the development of human induced pluripotent stem cell (hiPSC)-derived dopaminergic (DA) neurons. Following directed di...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Fang D,Qing Y,Yan S,Chen D,Yan SS

    更新日期:2016-10-11 00:00:00

  • Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming.

    abstract::Reprogramming of somatic cells produces induced pluripotent stem cells (iPSCs) that are invaluable resources for biomedical research. Here, we extended the previous transcriptome studies by performing RNA-seq on cells defined by a combination of multiple cellular surface markers. We found that transcriptome changes du...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Tanaka Y,Hysolli E,Su J,Xiang Y,Kim KY,Zhong M,Li Y,Heydari K,Euskirchen G,Snyder MP,Pan X,Weissman SM,Park IH

    更新日期:2015-06-09 00:00:00