Cancer Stem Cells: Notes for Authors.


:Stem Cell Reports frequently receives manuscripts dealing with the topic of cancer stem cells. Many of the submissions on this topic have major shortcomings in their content or limits to the conclusions that can be drawn from the results presented. The purpose of this Commentary is to highlight some of the underlying issues so that authors can enhance the strength of their research contributions.


Stem Cell Reports


Stem cell reports


Eaves CJ,Pera MF




Has Abstract


2020-02-11 00:00:00












  • Induced pluripotent stem cell generation-associated point mutations arise during the initial stages of the conversion of these cells.

    abstract::A large number of point mutations have been identified in induced pluripotent stem cell (iPSC) genomes to date. Whether these mutations are associated with iPSC generation is an important and controversial issue. In this study, we approached this critical issue in different ways, including an assessment of iPSCs versu...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Sugiura M,Kasama Y,Araki R,Hoki Y,Sunayama M,Uda M,Nakamura M,Ando S,Abe M

    更新日期:2014-01-02 00:00:00

  • Human Teratoma-Derived Hematopoiesis Is a Highly Polyclonal Process Supported by Human Umbilical Vein Endothelial Cells.

    abstract::Hematopoietic stem cells (HSCs) ensure a life-long regeneration of the blood system and are therefore an important source for transplantation and gene therapy. The teratoma environment supports the complex development of functional HSCs from human pluripotent stem cells, which is difficult to recapitulate in culture. ...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Philipp F,Selich A,Rothe M,Hoffmann D,Rittinghausen S,Morgan MA,Klatt D,Glage S,Lienenklaus S,Neuhaus V,Sewald K,Braun A,Schambach A

    更新日期:2018-11-13 00:00:00

  • Detecting Genetic Mosaicism in Cultures of Human Pluripotent Stem Cells.

    abstract::Genetic changes in human pluripotent stem cells (hPSCs) gained during culture can confound experimental results and potentially jeopardize the outcome of clinical therapies. Particularly common changes in hPSCs are trisomies of chromosomes 1, 12, 17, and 20. Thus, hPSCs should be regularly screened for such aberration...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Baker D,Hirst AJ,Gokhale PJ,Juarez MA,Williams S,Wheeler M,Bean K,Allison TF,Moore HD,Andrews PW,Barbaric I

    更新日期:2016-11-08 00:00:00

  • Direct reprogramming of human fibroblasts toward a cardiomyocyte-like state.

    abstract::Direct reprogramming of adult somatic cells into alternative cell types has been shown for several lineages. We previously showed that GATA4, MEF2C, and TBX5 (GMT) directly reprogrammed nonmyocyte mouse heart cells into induced cardiomyocyte-like cells (iCMs) in vitro and in vivo. However, GMT alone appears insufficie...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Fu JD,Stone NR,Liu L,Spencer CI,Qian L,Hayashi Y,Delgado-Olguin P,Ding S,Bruneau BG,Srivastava D

    更新日期:2013-08-22 00:00:00

  • Five transcription factors and FGF pathway inhibition efficiently induce erythroid differentiation in the epiblast.

    abstract::Primitive erythropoiesis follows a stereotypic developmental program of mesoderm ventralization and internalization, hemangioblast formation and migration, and erythroid lineage specification. Induction of erythropoiesis is inefficient in either ES/iPS cells in vitro or nonhemangioblast cell populations in vivo. Using...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Weng W,Sheng G

    更新日期:2014-03-06 00:00:00

  • Sox2-mediated regulation of adult neural crest precursors and skin repair.

    abstract::Nerve-derived neural crest cells are essential for regeneration in certain animals, such as newts. Here, we asked whether they play a similar role during mammalian tissue repair, focusing on Sox2-positive neural crest precursors in skin. In adult skin, Sox2 was expressed in nerve-terminal-associated neural crest precu...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Johnston AP,Naska S,Jones K,Jinno H,Kaplan DR,Miller FD

    更新日期:2013-06-04 00:00:00

  • A Quantitative Proteomic Analysis of Hemogenic Endothelium Reveals Differential Regulation of Hematopoiesis by SOX17.

    abstract::The in vitro derivation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) is complicated by the existence of multiple overlapping embryonic blood cell programs called primitive, erythromyeloid progenitor (EMP), and definitive. As HSCs are only generated during the definitive stage of hematopoiesis,...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Clarke RL,Robitaille AM,Moon RT,Keller G

    更新日期:2015-08-11 00:00:00

  • Functional Properties of Engineered Heart Slices Incorporating Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

    abstract::Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) hold great promise for cardiac studies, but their structural and functional immaturity precludes their use as faithful models of adult myocardium. Here we describe engineered heart slices (EHS), preparations of decellularized porcine myocardium rep...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Blazeski A,Lowenthal J,Zhu R,Ewoldt J,Boheler KR,Tung L

    更新日期:2019-05-14 00:00:00

  • Cell-to-Cell Adhesion and Neurogenesis in Human Cortical Development: A Study Comparing 2D Monolayers with 3D Organoid Cultures.

    abstract::Organoids (ORGs) are increasingly used as models of cerebral cortical development. Here, we compared transcriptome and cellular phenotypes between telencephalic ORGs and monolayers (MONs) generated in parallel from three biologically distinct induced pluripotent stem cell (iPSC) lines. Multiple readouts revealed incre...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Scuderi S,Altobelli GG,Cimini V,Coppola G,Vaccarino FM

    更新日期:2021-01-20 00:00:00

  • Enhanced Reconstitution of Human Erythropoiesis and Thrombopoiesis in an Immunodeficient Mouse Model with Kit(Wv) Mutations.

    abstract::In human-to-mouse xenograft models, reconstitution of human hematopoiesis is usually B-lymphoid dominant. Here we show that the introduction of homozygous Kit(Wv) mutations into C57BL/6.Rag2(null)Il2rg(null) mice with NOD-Sirpa (BRGS) strongly promoted human multi-lineage reconstitution. After xenotransplantation of h...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Yurino A,Takenaka K,Yamauchi T,Nunomura T,Uehara Y,Jinnouchi F,Miyawaki K,Kikushige Y,Kato K,Miyamoto T,Iwasaki H,Kunisaki Y,Akashi K

    更新日期:2016-09-13 00:00:00

  • HDAC1 and HDAC2 Modulate TGF-β Signaling during Endothelial-to-Hematopoietic Transition.

    abstract::The first hematopoietic stem and progenitor cells are generated during development from hemogenic endothelium (HE) through trans-differentiation. The molecular mechanisms underlying this endothelial-to-hematopoietic transition (EHT) remain poorly understood. Here, we explored the role of the epigenetic regulators HDAC...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Thambyrajah R,Fadlullah MZH,Proffitt M,Patel R,Cowley SM,Kouskoff V,Lacaud G

    更新日期:2018-04-10 00:00:00

  • Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System.

    abstract::Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nerv...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Rigamonti A,Repetti GG,Sun C,Price FD,Reny DC,Rapino F,Weisinger K,Benkler C,Peterson QP,Davidow LS,Hansson EM,Rubin LL

    更新日期:2016-06-14 00:00:00

  • Characterizing Direct-to-Consumer Stem Cell Businesses in the Southwest United States.

    abstract::There are currently hundreds of businesses across the United States offering direct-to-consumer stem cell treatments that have not been through regulatory approval by the Food and Drug Administration (FDA). Here, we provide a detailed characterization of nearly 170 stem cell businesses operating in the Southwest Unite...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Frow EK,Brafman DA,Muldoon A,Krum L,Williams P,Becker B,Nelson JP,Pritchett A

    更新日期:2019-08-13 00:00:00

  • In Vivo Generation of Engraftable Murine Hematopoietic Stem Cells by Gfi1b, c-Fos, and Gata2 Overexpression within Teratoma.

    abstract::Generation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) could potentially provide unlimited HSCs for clinical transplantation, a curative treatment for numerous blood diseases. However, to date, bona fide HSC generation has been largely unsuccessful in vitro. We have previously described proof...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Tsukada M,Ota Y,Wilkinson AC,Becker HJ,Osato M,Nakauchi H,Yamazaki S

    更新日期:2017-10-10 00:00:00

  • GATA6-AS1 Regulates GATA6 Expression to Modulate Human Endoderm Differentiation.

    abstract::Transcriptome analysis has uncovered a series of long noncoding RNAs (lncRNAs) transcribed during cell differentiation, but how lncRNA is integrated with known transcriptional regulatory network is poorly understood. Here, we utilize human definitive endoderm differentiation as a model system and decipher the function...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Yang J,Lu P,Li M,Yan C,Zhang T,Jiang W

    更新日期:2020-09-08 00:00:00

  • Enhanced Energetic State and Protection from Oxidative Stress in Human Myoblasts Overexpressing BMI1.

    abstract::The Polycomb group gene BMI1 is essential for efficient muscle regeneration in a mouse model of Duchenne muscular dystrophy, and its enhanced expression in adult skeletal muscle satellite cells ameliorates the muscle strength in this model. Here, we show that the impact of mild BMI1 overexpression observed in mouse mo...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Dibenedetto S,Niklison-Chirou M,Cabrera CP,Ellis M,Robson LG,Knopp P,Tedesco FS,Ragazzi M,Di Foggia V,Barnes MR,Radunovic A,Marino S

    更新日期:2017-08-08 00:00:00

  • Transgenesis and Genome Editing of Mouse Spermatogonial Stem Cells by Lentivirus Pseudotyped with Sendai Virus F Protein.

    abstract::Spermatogonial stem cells (SSCs) serve as a resource for producing genetically modified animals. However, genetic manipulation of SSCs has met with limited success. Here, we show efficient gene transfer into SSCs via a lentivirus (FV-LV) using a fusion protein (F), a Sendai virus (SV) envelope protein involved in viri...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Shinohara T,Kanatsu-Shinohara M

    更新日期:2020-03-10 00:00:00

  • Craniometaphyseal Dysplasia Mutations in ANKH Negatively Affect Human Induced Pluripotent Stem Cell Differentiation into Osteoclasts.

    abstract::We identified osteoclast defects in craniometaphyseal dysplasia (CMD) using an easy-to-use protocol for differentiating osteoclasts from human induced pluripotent stem cells (hiPSCs). CMD is a rare genetic bone disorder, characterized by life-long progressive thickening of craniofacial bones and abnormal shape of long...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Chen IP,Luxmi R,Kanaujiya J,Hao Z,Reichenberger EJ

    更新日期:2017-11-14 00:00:00

  • Human Pluripotent Stem Cell-Derived Cardiac Tissue-like Constructs for Repairing the Infarcted Myocardium.

    abstract::High-purity cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) are promising for drug development and myocardial regeneration. However, most hiPSC-derived CMs morphologically and functionally resemble immature rather than adult CMs, which could hamper their application. Here, we obtained h...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Li J,Minami I,Shiozaki M,Yu L,Yajima S,Miyagawa S,Shiba Y,Morone N,Fukushima S,Yoshioka M,Li S,Qiao J,Li X,Wang L,Kotera H,Nakatsuji N,Sawa Y,Chen Y,Liu L

    更新日期:2017-11-14 00:00:00

  • Targeted Repair of p47-CGD in iPSCs by CRISPR/Cas9: Functional Correction without Cleavage in the Highly Homologous Pseudogenes.

    abstract::Mutations in the NADPH oxidase, which is crucial for the respiratory burst in phagocytes, result in chronic granulomatous disease (CGD). The only curative treatment option for CGD patients, who suffer from severe infections, is allogeneic bone marrow transplantation. Over 90% of patients with mutations in the p47phox ...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Klatt D,Cheng E,Philipp F,Selich A,Dahlke J,Schmidt RE,Schott JW,Büning H,Hoffmann D,Thrasher AJ,Schambach A

    更新日期:2019-10-08 00:00:00

  • Context-Dependent Functions of NANOG Phosphorylation in Pluripotency and Reprogramming.

    abstract::The core pluripotency transcription factor NANOG is critical for embryonic stem cell (ESC) self-renewal and somatic cell reprogramming. Although NANOG is phosphorylated at multiple residues, the role of NANOG phosphorylation in ESC self-renewal is incompletely understood, and no information exists regarding its functi...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Saunders A,Li D,Faiola F,Huang X,Fidalgo M,Guallar D,Ding J,Yang F,Xu Y,Zhou H,Wang J

    更新日期:2017-05-09 00:00:00

  • Early depletion of primordial germ cells in zebrafish promotes testis formation.

    abstract::As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation. We applied morpholin...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Tzung KW,Goto R,Saju JM,Sreenivasan R,Saito T,Arai K,Yamaha E,Hossain MS,Calvert MEK,Orbán L

    更新日期:2015-01-13 00:00:00

  • Injury to the spinal cord niche alters the engraftment dynamics of human neural stem cells.

    abstract::The microenvironment is a critical mediator of stem cell survival, proliferation, migration, and differentiation. The majority of preclinical studies involving transplantation of neural stem cells (NSCs) into the CNS have focused on injured or degenerating microenvironments, leaving a dearth of information as to how N...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Sontag CJ,Uchida N,Cummings BJ,Anderson AJ

    更新日期:2014-04-18 00:00:00

  • Single-cell analysis of proxy reporter allele-marked epithelial cells establishes intestinal stem cell hierarchy.

    abstract::The recent development of targeted murine reporter alleles as proxies for intestinal stem cell activity has led to significant advances in our understanding of somatic stem cell hierarchies and dynamics. Analysis of these reporters has led to a model in which an indispensable reserve stem cell at the top of the hierar...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Li N,Yousefi M,Nakauka-Ddamba A,Jain R,Tobias J,Epstein JA,Jensen ST,Lengner CJ

    更新日期:2014-11-11 00:00:00

  • High-Level Precise Knockin of iPSCs by Simultaneous Reprogramming and Genome Editing of Human Peripheral Blood Mononuclear Cells.

    abstract::We have developed an improved episomal vector system for efficient generation of integration-free induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells. More recently, we reported that the use of an optimized CRISPR-Cas9 system together with a double-cut donor increases homology-directed repai...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Wen W,Cheng X,Fu Y,Meng F,Zhang JP,Zhang L,Li XL,Yang Z,Xu J,Zhang F,Botimer GD,Yuan W,Sun C,Cheng T,Zhang XB

    更新日期:2018-06-05 00:00:00

  • FAM122A Inhibits Erythroid Differentiation through GATA1.

    abstract::FAM122A is a highly conserved housekeeping gene, but its physiological and pathophysiological roles remain greatly elusive. Based on the fact that FAM122A is highly expressed in human CD71+ early erythroid cells, herein we report that FAM122A is downregulated during erythroid differentiation, while its overexpression ...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Chen J,Zhou Q,Liu MH,Yang YS,Wang YQ,Huang Y,Chen GQ

    更新日期:2020-09-08 00:00:00

  • Developmental IL-6 Exposure Favors Production of PDGF-Responsive Multipotential Progenitors at the Expense of Neural Stem Cells and Other Progenitors.

    abstract::Interleukin-6 (IL-6) is increased in maternal serum and amniotic fluid of children subsequently diagnosed with autism spectrum disorders. However, it is not clear how increased IL-6 alters brain development. Here, we show that IL-6 increases the prevalence of a specific platelet-derived growth factor (PDGF)-responsive...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Kumari E,Velloso FJ,Nasuhidehnavi A,Somasundaram A,Savanur VH,Buono KD,Levison SW

    更新日期:2020-05-12 00:00:00

  • Sox100B Regulates Progenitor-Specific Gene Expression and Cell Differentiation in the Adult Drosophila Intestine.

    abstract::Robust production of terminally differentiated cells from self-renewing resident stem cells is essential to maintain proper tissue architecture and physiological functions, especially in high-turnover tissues. However, the transcriptional networks that precisely regulate cell transition and differentiation are poorly ...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Meng FW,Rojas Villa SE,Biteau B

    更新日期:2020-02-11 00:00:00

  • Origins of Neural Progenitor Cell-Derived Axons Projecting Caudally after Spinal Cord Injury.

    abstract::Neural progenitor cells (NPCs) transplanted into sites of spinal cord injury (SCI) extend large numbers of axons into the caudal host spinal cord. We determined the precise locations of neurons in the graft that extend axons into the caudal host spinal cord using AAV9-Cre-initiated retrograde tracing into floxed-TdTom...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Lu P,Gomes-Leal W,Anil S,Dobkins G,Huie JR,Ferguson AR,Graham L,Tuszynski M

    更新日期:2019-07-09 00:00:00

  • Modeling Developmental and Tumorigenic Aspects of Trilateral Retinoblastoma via Human Embryonic Stem Cells.

    abstract::Human embryonic stem cells (hESCs) provide a platform for studying human development and understanding mechanisms underlying diseases. Retinoblastoma-1 (RB1) is a key regulator of cell cycling, of which biallelic inactivation initiates retinoblastoma, the most common congenital intraocular malignancy. We developed a m...

    journal_title:Stem cell reports

    pub_type: 杂志文章


    authors: Avior Y,Lezmi E,Yanuka D,Benvenisty N

    更新日期:2017-05-09 00:00:00