Genetic Analysis of the IncX4 Plasmids: Implications for a Unique Pattern in the mcr-1 Acquisition.

Abstract:

:IncX4 plasmids are associated with the dissemination of the mcr-1 genes in Enterobacteriaceae. We screened IncX4 plasmids among 2,470 isolates of Enterobacteriaceae and determined the mcr-1 positive isolates. Forty-three isolates were observed to carry IncX4 type plasmid, among which 13 were identified to carry mcr-1 gene. Three representative mcr-1-positive IncX4 plasmids were selected for high-throughput sequencing. Comparative genomics showed that the mcr-1-carrying IncX4 plasmids exhibit remarkable similarity in the backbone, and the major distinction lies in the region containing mcr-1. The major variable regions of all the IncX4 plasmids were fully characterized by PCR-RFLP. The results revealed that the mcr-1 was located on the Variable Region I of IncX4 plasmids in 11 E. coli isolates. Among them, nine E. coli strains possess an epidemic pCSZ4-like IncX4 plasmid containing mcr-1. ISApl1 was presumably involved in the transposition of the mcr-1 cassette and then was lost. Similar genetic contexts were found in different plasmids, even the E. coli chromosome, implying the acquisition of mcr-1 by a unique common mechanism.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Sun J,Fang LX,Wu Z,Deng H,Yang RS,Li XP,Li SM,Liao XP,Feng Y,Liu YH

doi

10.1038/s41598-017-00095-x

subject

Has Abstract

pub_date

2017-03-24 00:00:00

pages

424

issue

1

issn

2045-2322

pii

10.1038/s41598-017-00095-x

journal_volume

7

pub_type

杂志文章
  • Cardiac endothelial cell-derived exosomes induce specific regulatory B cells.

    abstract::The mechanism of immune tolerance is to be further understood. The present study aims to investigate the role of the Cardiac endothelial cell (CEC)-derived exosomes in the induction of regulatory B cells. In this study, CECs were isolated from the mouse heart. Exosomes were purified from the culture supernatant of the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep07583

    authors: Song J,Chen X,Wang M,Xing Y,Zheng Z,Hu S

    更新日期:2014-12-23 00:00:00

  • The Japanese herbal medicine Hangeshashinto enhances oral keratinocyte migration to facilitate healing of chemotherapy-induced oral ulcerative mucositis.

    abstract::Chemotherapy often induces oral ulcerative mucositis (OUM) in patients with cancer, characterized by severe painful inflammation. Mouth-washing with the Japanese herbal medicine hangeshashinto (HST) ameliorates chemotherapy-induced OUM in patients with colorectal cancer. Previously, we demonstrated that HST decreased ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-57192-2

    authors: Miyano K,Eto M,Hitomi S,Matsumoto T,Hasegawa S,Hirano A,Nagabuchi K,Asai N,Uzu M,Nonaka M,Omiya Y,Kaneko A,Ono K,Fujii H,Higami Y,Kono T,Uezono Y

    更新日期:2020-01-17 00:00:00

  • Polymorphism A118G of opioid receptor mu 1 (OPRM1) is associated with emergence of suicidal ideation at antidepressant onset in a large naturalistic cohort of depressed outpatients.

    abstract::Antidepressants have been the object of an international controversy for about thirty years. Some patients are inclined to develop suicidal ideation (SI) at antidepressant onset; this phenomenon is known as Treatment Emergent Suicidal Ideation (TESI), and it has conducted regulatory bodies to prompt warnings on antide...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1038/s41598-019-39622-3

    authors: Nobile B,Ramoz N,Jaussent I,Gorwood P,Olié E,Castroman JL,Guillaume S,Courtet P

    更新日期:2019-02-22 00:00:00

  • Generating trunk neural crest from human pluripotent stem cells.

    abstract::Neural crest cells (NCC) are stem cells that generate different lineages, including neuroendocrine, melanocytic, cartilage, and bone. The differentiation potential of NCC varies according to the level from which cells emerge along the neural tube. For example, only anterior "cranial" NCC form craniofacial bone, wherea...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19727

    authors: Huang M,Miller ML,McHenry LK,Zheng T,Zhen Q,Ilkhanizadeh S,Conklin BR,Bronner ME,Weiss WA

    更新日期:2016-01-27 00:00:00

  • Non-invasive Potential Circulating mRNA Markers for Colorectal Adenoma Using Targeted Sequencing.

    abstract::We have developed an optimized protocol for plasma targeted mRNA sequencing in our previous study. Here, we performed plasma targeted mRNA sequencing for 40 colorectal adenoma patients and 39 colonoscopy-proven normal controls in order to find potential circulating mRNA markers for colorectal adenoma. Results showed t...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-49445-x

    authors: Xue VW,Cheung MT,Chan PT,Luk LLY,Lee VH,Au TC,Yu AC,Cho WCS,Tsang HFA,Chan AK,Wong SCC

    更新日期:2019-09-10 00:00:00

  • Effect of acute pesticide exposure on bee spatial working memory using an analogue of the radial-arm maze.

    abstract::Pesticides, including neonicotinoids, typically target pest insects by being neurotoxic. Inadvertent exposure to foraging insect pollinators is usually sub-lethal, but may affect cognition. One cognitive trait, spatial working memory, may be important in avoiding previously-visited flowers and other spatial tasks such...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38957

    authors: Samuelson EE,Chen-Wishart ZP,Gill RJ,Leadbeater E

    更新日期:2016-12-13 00:00:00

  • Distribution and characteristics of rodent picornaviruses in China.

    abstract::Rodents are important reservoir hosts of many important zoonotic viruses. The family Picornaviridae contains clinically important pathogens that infect humans and animals, and increasing numbers of rodent picornaviruses have recently been associated with zoonoses. We collected 574 pharyngeal and anal swab specimens fr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep34381

    authors: Du J,Lu L,Liu F,Su H,Dong J,Sun L,Zhu Y,Ren X,Yang F,Guo F,Liu Q,Wu Z,Jin Q

    更新日期:2016-09-29 00:00:00

  • Lévy Walk Navigation in Complex Networks: A Distinct Relation between Optimal Transport Exponent and Network Dimension.

    abstract::We investigate, for the first time, navigation on networks with a Lévy walk strategy such that the step probability scales as pij ~ dij(-α), where dij is the Manhattan distance between nodes i and j, and α is the transport exponent. We find that the optimal transport exponent α(opt) of such a diffusion process is dete...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep17309

    authors: Weng T,Small M,Zhang J,Hui P

    更新日期:2015-11-25 00:00:00

  • The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata.

    abstract::SeviL is a recently isolated lectin found to bind to the linear saccharides of the ganglioside GM1b (Neu5Ac[Formula: see text](2-3)Gal[Formula: see text](1-3)GalNAc[Formula: see text](1-4)Gal[Formula: see text](1-4)Glc) and its precursor, asialo-GM1 (Gal[Formula: see text](1-3)GalNAc[Formula: see text](1-4)Gal[Formula...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-78926-7

    authors: Kamata K,Mizutani K,Takahashi K,Marchetti R,Silipo A,Addy C,Park SY,Fujii Y,Fujita H,Konuma T,Ikegami T,Ozeki Y,Tame JRH

    更新日期:2020-12-16 00:00:00

  • FGF21 ameliorates the neurocontrol of blood pressure in the high fructose-drinking rats.

    abstract::Fibroblast growth factor-21 (FGF21) is closely related to various metabolic and cardiovascular disorders. However, the direct targets and mechanisms linking FGF21 to blood pressure control and hypertension are still elusive. Here we demonstrated a novel regulatory function of FGF21 in the baroreflex afferent pathway (...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep29582

    authors: He JL,Zhao M,Xia JJ,Guan J,Liu Y,Wang LQ,Song DX,Qu MY,Zuo M,Wen X,Yu X,Huo R,Pan ZW,Ban T,Zhang Y,Zhu JX,Shou W,Qiao GF,Li BY

    更新日期:2016-07-08 00:00:00

  • PD-L1 expression in lung adenosquamous carcinomas compared with the more common variants of non-small cell lung cancer.

    abstract::Lung adenosquamous cell carcinomas (ASCs) is a rare variant of NSCLC with a poorer prognosis and fewer treatment option than the more common variants. PD-L1 expression is reported to be the predictor of clinical response in trials of NSCLC. In our study, PD-L1 expression was evaluated via immunohistochemistry using a ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep46209

    authors: Shi X,Wu S,Sun J,Liu Y,Zeng X,Liang Z

    更新日期:2017-04-07 00:00:00

  • Axial plane optical microscopy.

    abstract::We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More im...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep07253

    authors: Li T,Ota S,Kim J,Wong ZJ,Wang Y,Yin X,Zhang X

    更新日期:2014-12-01 00:00:00

  • Phenology, mobility and behaviour of the arcto-alpine species Boloria napaea in its arctic habitat.

    abstract::Arctic and alpine environments present extreme, but different, challenges to survival. We therefore studied the ecological adaptation of the arctic-alpine fritillary Boloria napaea in northern Sweden and compared these results with the eastern Alps. Using mark-release-recapture, we analysed phenology, mobility, activi...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-40508-7

    authors: Ehl S,Holzhauer SIJ,Ryrholm N,Schmitt T

    更新日期:2019-03-07 00:00:00

  • Tuning bandgap and surface wettability of NiFe2O4 driven by phase transition.

    abstract::Stress variation induced bandgap tuning and surface wettability switching of spinel nickel ferrite (NiFe2O4, NFO) films were demonstrated and directly driven by phase transition via a post-annealing process. Firstly, the as-deposited NFO films showed hydrophilic surface with water contact angle (CA) value of 80 ± 1°. ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-19319-9

    authors: Tong SK,Chi PW,Kung SH,Wei DH

    更新日期:2018-01-22 00:00:00

  • Production and Characterization of Superhydrophobic and Antibacterial Coated Fabrics Utilizing ZnO Nanocatalyst.

    abstract::Dirt and microorganisms are the major problems in textiles which can generate unpleasant odor during their growth. Here, zinc oxide (ZnO) nanoparticles prepared by sol-gel method were loaded on the cotton fabrics using spin coating technique to enhance their antimicrobial properties and water repellency. The effects o...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-22324-7

    authors: Shaban M,Mohamed F,Abdallah S

    更新日期:2018-03-02 00:00:00

  • Interferon-alpha therapy for refractory kaposiform hemangioendothelioma: a single-center experience.

    abstract::Kaposiform hemangioendothelioma (KHE) is a relatively rare vascular tumor with an aggressive and infiltrating nature. Previous studies have revealed an exclusive relationship between KHE and Kasabach-Merritt Phenomenon (KMP), which is associated with high morbidity and mortality. No universally accepted treatment moda...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章

    doi:10.1038/srep36261

    authors: Wu HW,Wang X,Zhang L,Zhao HG,Wang YA,Su LX,Fan XD,Zheng JW

    更新日期:2016-10-31 00:00:00

  • Biosynthesis of CuO nanoparticles using aqueous extract of herbal tea (Stachys Lavandulifolia) flowers and evaluation of its catalytic activity.

    abstract::Plant derived biogenic synthesis of nanoparticles (NP) has been the recent trend in material science as featured sustainable catalysts. A great deal of the current nanocatalytic research has been oriented on the bio-inspired green catalysts based on their wide applicability. In this context, CuO NPs are synthesized fo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-81320-6

    authors: Veisi H,Karmakar B,Tamoradi T,Hemmati S,Hekmati M,Hamelian M

    更新日期:2021-01-21 00:00:00

  • Multiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidics.

    abstract::Isolation of low abundance proteins or rare cells from complex mixtures, such as blood, is required for many diagnostic, therapeutic and research applications. Current affinity-based protein or cell separation methods use binary 'bind-elute' separations and are inefficient when applied to the isolation of multiple low...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep23589

    authors: Sarkar A,Hou HW,Mahan AE,Han J,Alter G

    更新日期:2016-03-30 00:00:00

  • Anaerobiosis influences virulence properties of Pseudomonas aeruginosa cystic fibrosis isolates and the interaction with Staphylococcus aureus.

    abstract::The airways of individuals with cystic fibrosis (CF) are abundantly colonised by Staphylococcus aureus and Pseudomonas aeruginosa. Co-infecting hypoxic regions of static mucus within CF airways, together with decreases in pulmonary function, mucus plugging and oxygen consumption by host neutrophils gives rise to regio...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-42952-x

    authors: Pallett R,Leslie LJ,Lambert PA,Milic I,Devitt A,Marshall LJ

    更新日期:2019-05-01 00:00:00

  • Preoperative prognostic nutritional index is a powerful predictor of prognosis in patients with stage III ovarian cancer.

    abstract::Many established inflammation- and nutrition-related factors have been investigated as potential independent prognostic factors in various cancers, including the C-reactive protein/albumin ratio (CAR), lymphocyte/monocyte ratio (LMR), modified Glasgow prognostic score (mGPS), body mass index (BMI), and prognostic nutr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10328-8

    authors: Zhang W,Ye B,Liang W,Ren Y

    更新日期:2017-08-25 00:00:00

  • Verteporfin inhibits growth of human glioma in vitro without light activation.

    abstract::Verteporfin (VP), a light-activated drug used in photodynamic therapy for the treatment of choroidal neovascular membranes, has also been shown to be an effective inhibitor of malignant cells. Recently, studies have demonstrated that, even without photo-activation, VP may still inhibit certain tumor cell lines, includ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-07632-8

    authors: Al-Moujahed A,Brodowska K,Stryjewski TP,Efstathiou NE,Vasilikos I,Cichy J,Miller JW,Gragoudas E,Vavvas DG

    更新日期:2017-08-08 00:00:00

  • Optimal donor for severe aplastic anemia patient requiring allogeneic hematopoietic stem cell transplantation: A large-sample study from China.

    abstract::HLA-haploidentical hematopoietic stem cell transplantation (HSCT) may be an option for severe aplastic anemia (SAA) patients. However, to date, no large-sample studies have been performed to determine which types of SAA patients are suitable for HLA-haploidentical HSCT. We retrospectively studied 189 consecutive patie...

    journal_title:Scientific reports

    pub_type: 杂志文章,多中心研究

    doi:10.1038/s41598-018-20853-9

    authors: Zeng Y,Wang S,Wang J,Liu L,Su Y,Lu Z,Zhang X,Zhang Y,Zhong JF,Peng L,Liu Q,Lu Y,Gao L,Zhang X

    更新日期:2018-02-06 00:00:00

  • Using proximity extension proteomics assay to discover novel biomarkers associated with circulating leptin levels in patients with type 2 diabetes.

    abstract::We aimed to discover novel associations between leptin and circulating proteins which could link leptin to the development of cardiovascular disease in patients with type 2 diabetes (T2DM). In a discovery phase, we investigated associations between 88 plasma proteins, assessed with a proximity extension assay, and pla...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-69473-2

    authors: Vavruch C,Nowak C,Feldreich T,Östgren CJ,Sundström J,Söderberg S,Lind L,Nyström F,Ärnlöv J

    更新日期:2020-08-04 00:00:00

  • Anticandidal and In vitro Anti-Proliferative Activity of Sonochemically synthesized Indium Tin Oxide Nanoparticles.

    abstract::The present work demonstrates the synthesis, characterization and biological activities of different concentrations of tin doped indium oxide nanoparticles (Sn doped In2O3 NPs), i.e., (Sn/In = 5%, 10% and 15%). We have synthesized different size (38.11 nm, 18.46 nm and 10.21 nm) of Sn doped In2O3 NPs. by using an ultr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60295-w

    authors: Rehman S,Asiri SM,Khan FA,Jermy BR,Ravinayagam V,Alsalem Z,Jindan RA,Qurashi A

    更新日期:2020-02-24 00:00:00

  • Comprehensive analysis of The Cancer Genome Atlas reveals a unique gene and non-coding RNA signature of fibrolamellar carcinoma.

    abstract::Fibrolamellar carcinoma (FLC) is a unique liver cancer primarily affecting young adults and characterized by a fusion event between DNAJB1 and PRKACA. By analyzing RNA-sequencing data from The Cancer Genome Atlas (TCGA) for >9,100 tumors across ~30 cancer types, we show that the DNAJB1-PRKACA fusion is specific to FLC...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep44653

    authors: Dinh TA,Vitucci EC,Wauthier E,Graham RP,Pitman WA,Oikawa T,Chen M,Silva GO,Greene KG,Torbenson MS,Reid LM,Sethupathy P

    更新日期:2017-03-17 00:00:00

  • Tailoring thermal conductivity via three-dimensional porous alumina.

    abstract::Three-dimensional anodic alumina templates (3D-AAO) are an astonishing framework with open highly ordered three-dimensional skeleton structures. Since these templates are architecturally different from conventional solids or porous templates, they teem with opportunities for engineering thermal properties. By establis...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep38595

    authors: Abad B,Maiz J,Ruiz-Clavijo A,Caballero-Calero O,Martin-Gonzalez M

    更新日期:2016-12-09 00:00:00

  • Nonmonotonic particle-size-dependence of magnetoelectric coupling in strained nanosized particles of BiFeO3.

    abstract::Using high resolution powder x-ray and neutron diffraction experiments, we determined the off-centered displacement of the ions within a unit cell and magnetoelectric coupling in nanoscale BiFeO3 (≈20-200 nm). We found that both the off-centered displacement of the ions and magnetoelectric coupling exhibit nonmonotoni...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21803-1

    authors: Goswami S,Bhattacharya D,Ghosh CK,Ghosh B,Kaushik SD,Siruguri V,Krishna PSR

    更新日期:2018-02-27 00:00:00

  • Nano-sized Superlattice Clusters Created by Oxygen Ordering in Mechanically Alloyed Fe Alloys.

    abstract::Creating and maintaining precipitates coherent with the host matrix, under service conditions is one of the most effective approaches for successful development of alloys for high temperature applications; prominent examples include Ni- and Co-based superalloys and Al alloys. While ferritic alloys are among the most i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep11772

    authors: Hu YJ,Li J,Darling KA,Wang WY,VanLeeuwen BK,Liu XL,Kecskes LJ,Dickey EC,Liu ZK

    更新日期:2015-07-02 00:00:00

  • A key process of the nonstationary relationship between ENSO and the Western Pacific teleconnection pattern.

    abstract::Recent studies have discovered an intriguing nonstationary relationship between El Ninõ-Southern Oscillation (ENSO) and the Western Pacific (WP) teleconnection pattern, one of the most prominent winter atmospheric circulation patterns in the North Pacific, with a regime-dependent interdecadal modulation of significant...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-27906-z

    authors: Park YH,Kim BM,Pak G,Yamamoto M,Vivier F,Durand I

    更新日期:2018-06-22 00:00:00

  • Overexpression of LINC00152 correlates with poor patient survival and knockdown impairs cell proliferation in lung cancer.

    abstract::We employed RNA sequencing analysis to reveal dysregulated lncRNAs in lung cancer utilizing 461 lung adenocarcinomas and 156 normal lung tissues from 3 separate cohorts. We found that LINC00152 was highly overexpressed in lung tumors as compared to their adjacent normal tissues. Patients with high LINC00152 expression...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-03043-x

    authors: Feng S,Zhang J,Su W,Bai S,Xiao L,Chen X,Lin J,Reddy RM,Chang AC,Beer DG,Chen G

    更新日期:2017-06-07 00:00:00