Abstract：
:Isolation of low abundance proteins or rare cells from complex mixtures, such as blood, is required for many diagnostic, therapeutic and research applications. Current affinity-based protein or cell separation methods use binary 'bind-elute' separations and are inefficient when applied to the isolation of multiple low-abundance proteins or cell types. We present a method for rapid and multiplexed, yet inexpensive, affinity-based isolation of both proteins and cells, using a size-coded mixture of multiple affinity-capture microbeads and an inertial microfluidic particle sorter device. In a single binding step, different targets-cells or proteins-bind to beads of different sizes, which are then sorted by flowing them through a spiral microfluidic channel. This technique performs continuous-flow, high throughput affinity-separation of milligram-scale protein samples or millions of cells in minutes after binding. We demonstrate the simultaneous isolation of multiple antibodies from serum and multiple cell types from peripheral blood mononuclear cells or whole blood. We use the technique to isolate low abundance antibodies specific to different HIV antigens and rare HIV-specific cells from blood obtained from HIV+ patients.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sarkar A,Hou HW,Mahan AE,Han J,Alter Gdoi
10.1038/srep23589subject
Has Abstractpub_date
2016-03-30 00:00:00pages
23589issn
2045-2322pii
srep23589journal_volume
6pub_type
杂志文章abstract：:In this work, four water-soluble polythiophene derivatives (PT, PT-DDA, PT-ADA, and PT-ADA-PPR) with different pendant moieties were synthesized via oxidative copolymerization by FeCl3. By increasing the hydrophobic ability of side chain moieties, there is a gradually blue shift for the maximum absorption wavelength a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07617
更新日期：2015-01-05 00:00:00
abstract：:The ERK signalling pathway regulates key cell fate decisions in the intestinal epithelium and is frequently dysregulated in colorectal cancers (CRCs). Variations in the dynamics of ERK activation can induce different biological outcomes and are regulated by multiple mechanisms, including activation of negative feedbac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20176-9
更新日期：2018-01-29 00:00:00
abstract：:The ultimate goal of epileptology is the complete abolishment of epileptic seizures. This might be achieved by a system that predicts seizure onset combined with a system that interferes with the process that leads to the onset of a seizure. Seizure prediction remains, as of yet, unresolved in absence-epilepsy, due to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02626-y
更新日期：2017-05-29 00:00:00
abstract：:Aluminum-based quasicrystals typically form across narrow composition ranges within binary to quaternary alloys, which makes their fabrication and characterization challenging. Here, we use combinatorial approaches together with fast characterization techniques to study a wide compositional range including known quasi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-43666-w
更新日期：2019-05-09 00:00:00
abstract：:After spinal cord injury (SCI) chronic inflammation hampers regeneration. Influencing the local microenvironment after SCI may provide a strategy to modulate inflammation and the immune response. The objectives of this work were to determine whether bone or spinal cord derived ECM hydrogels can deliver human mesenchym...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50367-x
更新日期：2019-09-30 00:00:00
abstract：:Transcript levels are the primary factor determining protein levels, but for the majority of genes, fold changes in transcript levels are larger than the corresponding changes in protein levels, a phenomenon that is termed "protein homeostasis". However, this phenomenon is not well characterized in the context of envi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44305-0
更新日期：2019-05-24 00:00:00
abstract：:Bruton tyrosine kinase (BTK) is a key enzyme in B-cell development whose improper regulation causes severe immunodeficiency diseases. Design of selective BTK therapeutics would benefit from improved, in-silico structural modeling of the kinase's solution ensemble. However, this remains challenging due to the immense c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10697-0
更新日期：2017-11-15 00:00:00
abstract：:mRNAs lacking 5' untranslated regions (leaderless mRNAs) are molecular relics of an ancient translation initiation pathway. Nevertheless, they still represent a significant portion of transcriptome in some taxons, including a number of eukaryotic species. In bacteria and archaea, the leaderless mRNAs can bind non-diss...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37905
更新日期：2016-11-28 00:00:00
abstract：:Infection of humans with Chlamydia trachomatis, a bacterial pathogen with a unique intracellular replication cycle, may cause a variety of clinical manifestations. These are linked to various serovars of the pathogen; trachoma to serovars A-C, oculogenital infections to serovars D-K, and lymphogranuloma venereum to se...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54886-5
更新日期：2019-12-04 00:00:00
abstract：:Nedd4-2 (NEDD4L in humans) is a ubiquitin protein ligase best known for its role in regulating ion channel internalization and turnover. Nedd4-2 deletion in mice causes perinatal lethality associated with increased epithelial sodium channel (ENaC) expression in lung and kidney. Abundant data suggest that Nedd4-2 plays...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep32957
更新日期：2016-09-08 00:00:00
abstract：:The purpose of this work is to make a case for epidemiological models with fractional exponent in the contribution of sub-populations to the incidence rate. More specifically, we question the standard assumption in the literature on epidemiological models, where the incidence rate dictating propagation of infections i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77849-7
更新日期：2020-11-30 00:00:00
abstract：:Pathologic diagnoses mainly depend on visual scoring by pathologists, a process that can be time-consuming, laborious, and susceptible to inter- and/or intra-observer variations. This study proposes a novel method to enhance pathologic scoring of renal allograft rejection. A fully automated system using a convolutiona...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41479-5
更新日期：2019-03-26 00:00:00
abstract：:Andrographolide is a traditional herb medicine, widely used in Asia for conditions involving inflammation. The andrographlide-lipoic acid conjugate, AL-1, has been found being able to alleviate inflammation in our previous reports. Although the anti-inflammatory activity of AL-1 contributes to its cytoprotective effec...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29716
更新日期：2016-07-20 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-59025-z
更新日期：2020-02-07 00:00:00
abstract：:Global climate change has resulted in a warmer Arctic, with projections indicating accelerated modifications to permafrost in the near future. The thermal, hydrological, and mechanical physics of permafrost thaw have been hypothesized to couple in a complex fashion but data collection efforts to study these feedbacks ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29292-y
更新日期：2018-07-19 00:00:00
abstract：:Bone marrow mesenchymal stem cells (BM-MSCs) are used in tissue engineering because of their migration characters. However, BM-MSCs have limitations in terms of reaching injuries and self-renewal. Therefore, enhancement of BM-MSC migration is important for therapeutic applications. Here, we assessed whether galectin-3...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26577
更新日期：2016-05-24 00:00:00
abstract：:Phytoene desaturases catalyse from two to six desaturation reactions on phytoene, generating a large diversity of molecules that can then be cyclised and produce, depending on the organism, many different carotenoids. We constructed a phylogenetic tree of a subset of phytoene desaturases from the CrtI family for which...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77876-4
更新日期：2020-12-03 00:00:00
abstract：:This study investigated a rabbit model of autologous simple oral mucosal epithelium transplantation (SOMET) for limbal stem cell deficiency (LSCD). LSCD was created in the SOMET group and the Control group. In the SOMET group, oral mucosa harvested from the buccal region was treated with dispase, cut into small pieces...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54571-7
更新日期：2019-12-02 00:00:00
abstract：:Brønsted-Lewis acidic ionic liquids (ILs) were applied to catalyze cellobiose to prepare levulinic acid (LA) in one pot under hydrothermal conditions. Under the optimum conditions, the highest LA yield of 67.51% was obtained when low [HO3S-(CH2)3-mim]Cl-FeCl3 (molar fraction of FeCl3 x = 0.60) was used. This indicated...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-38051-y
更新日期：2019-02-12 00:00:00
abstract：:Methods to repair bone defects arising from trauma, resection, or disease, continue to be sought after. Cyclic mechanical loading is well established to influence bone (re)modelling activity, in which bone formation and resorption are correlated to micro-scale strain. Based on this, the application of mechanical stimu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81132-8
更新日期：2021-01-21 00:00:00
abstract：:The ends of eukaryotic chromosomes are capped by telomeres which consist of tandem G-rich DNA repeats stabilized by the shelterin protein complex. Telomeres shorten progressively in most normal cells due to the end replication problem. In more than 85% of cancers however, the telomere length is maintained by telomeras...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06391
更新日期：2014-09-29 00:00:00
abstract：:The dorsal anterior cingulate cortex (dACC) has been identified as a core region affected by many disorders, representing a promising target for neuromodulation. High Definition-transcranial Direct Current Stimulation (HD-tDCS) is a non-invasive neuromodulation technique that has already shown promising outcomes and h...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-018-22730-x
更新日期：2018-03-13 00:00:00
abstract：:The strong electronegativity of O dictates that the ground state of singlet CO has positively charged C and negatively charged O, in agreement with ab initio charge analysis, but in disagreement with the dipole direction. Though this unusual phenomenon has been fairly studied, the study of electrostatic potential (EP)...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16307
更新日期：2015-11-06 00:00:00
abstract：:Retrotransposons can represent half of eukaryotic genomes. Retrotransposon dysregulation destabilizes genomes and has been linked to various human diseases. Emerging regulators of retromobility include RNA-DNA hybrid-containing structures known as R-loops. Accumulation of these structures at the transposons of yeast 1...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73018-y
更新日期：2020-09-29 00:00:00
abstract：:Research on Giardia lamblia has accumulated large information about its molecular cell biology and infection biology. However, giardiasis is still one of the commonest parasitic diarrheal diseases affecting humans. Additionally, an alarming increase in cases refractory to conventional treatment has been reported in lo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-45529-w
更新日期：2019-06-20 00:00:00
abstract：:Carbonic anhydrase plays a key role in CO2 transport, acid-base and ion regulation and metabolic processes in vertebrates. While several carbonic anhydrase isoforms have been identified in numerous vertebrate species, basal lineages such as the cyclostomes have remained largely unexamined. Here we investigate the repe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33954
更新日期：2016-10-05 00:00:00
abstract：:Some studies have demonstrated interactions of AD-risk single nucleotide polymorphisms (SNPs) in non-APOE regions with APOE genotype. Nevertheless, no study reported interactions of expression quantitative trait locus (eQTL) for APOE with APOE genotype. In present study, we included 9286 unrelated AD patients and 8479...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26398-1
更新日期：2018-05-23 00:00:00
abstract：:Here we report a photonic annealing process for yttria-stabilized zirconia films, which are one of the most well-known solid-state electrolytes for solid oxide fuel cells (SOFCs). Precursor films were coated using a wet-chemical method with a simple metal-organic precursor solution and directly annealed at standard pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12438-9
更新日期：2017-09-29 00:00:00
abstract：:Efforts for tissue engineering vascular grafts focuses on the tunica media and intima, although the tunica adventitia serves as the primary structural support for blood vessels. In surgery, during endarterectomies, surgeons can strip the vessel, leaving the adventitia as the main strength layer to close the vessel. He...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-21681-7
更新日期：2018-02-19 00:00:00
abstract：:Corals access inorganic seawater nutrients through their autotrophic endosymbiotic dinoflagellates, but also capture planktonic prey through heterotrophic feeding. Correlating NanoSIMS and TEM imaging, we visualized and quantified the subcellular fate of autotrophic and heterotrophic C and N in the coral Stylophora pi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31094-1
更新日期：2018-08-23 00:00:00
