Abstract:
:Neural crest cells (NCC) are stem cells that generate different lineages, including neuroendocrine, melanocytic, cartilage, and bone. The differentiation potential of NCC varies according to the level from which cells emerge along the neural tube. For example, only anterior "cranial" NCC form craniofacial bone, whereas solely posterior "trunk" NCC contribute to sympathoadrenal cells. Importantly, the isolation of human fetal NCC carries ethical and scientific challenges, as NCC induction typically occur before pregnancy is detectable. As a result, current knowledge of NCC biology derives primarily from non-human organisms. Important differences between human and non-human NCC, such as expression of HNK1 in human but not mouse NCC, suggest a need to study human NCC directly. Here, we demonstrate that current protocols to differentiate human pluripotent stem cells (PSC) to NCC are biased toward cranial NCC. Addition of retinoic acid drove trunk-related markers and HOX genes characteristic of a posterior identity. Subsequent treatment with bone morphogenetic proteins (BMPs) enhanced differentiation to sympathoadrenal cells. Our approach provides methodology for detailed studies of human NCC, and clarifies roles for retinoids and BMPs in the differentiation of human PSC to trunk NCC and to sympathoadrenal lineages.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Huang M,Miller ML,McHenry LK,Zheng T,Zhen Q,Ilkhanizadeh S,Conklin BR,Bronner ME,Weiss WAdoi
10.1038/srep19727subject
Has Abstractpub_date
2016-01-27 00:00:00pages
19727issn
2045-2322pii
srep19727journal_volume
6pub_type
杂志文章abstract::A biofilm has a unique structure composed of microorganisms, extracellular polymeric substances (EPSs), etc., and it is layered on a substrate in water. In material science, it is important to detect the biofilm formed on a surface to prevent biofouling. EPSs, the major component of the biofilm, mainly consist of poly...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44167-6
更新日期:2019-05-30 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-62685-6
更新日期:2020-04-02 00:00:00
abstract::A unique combination of useful properties in boron-carbide, such as extreme hardness, excellent fracture toughness, a low density, a high melting point, thermoelectricity, semi-conducting behavior, catalytic activity and a remarkably good chemical stability, makes it an ideal material for a wide range of technological...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep19330
更新日期:2016-01-18 00:00:00
abstract::To improve the performance of solar photovoltaic devices one should mitigate three types of losses: optical, electrical and thermal. However, further reducing the optical and electrical losses in modern photovoltaic devices is becoming increasingly costly. Therefore, there is a rising interest in minimizing the therma...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31257-0
更新日期:2018-09-03 00:00:00
abstract::This prospective randomized double-masked study investigated the effects of 20 mg lutein supplementation with two different capsules (beeswax or glycerol fatty acid esters) for 6 months on the fellow eyes of 39 Japanese patients with unilateral age-related macular degeneration, and assessed the factors associated with...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-020-62483-0
更新日期:2020-04-06 00:00:00
abstract::The mammalian Major Histocompatibility Complex (MHC) region contains several gene families characterized by highly polymorphic loci with extensive nucleotide diversity, copy number variation of paralogous genes, and long repetitive sequences. This structural complexity has made it difficult to construct a reliable ref...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45518
更新日期:2017-03-31 00:00:00
abstract::Here, we combine elicitors and transcriptomics to investigate the inducible biosynthesis of the ginsenoside from the Panax quinquefolium. Treatment of P. quinquefolium adventitious root with methyl jasmonate (MJ) results in an increase in ginsenoside content (43.66 mg/g compared to 8.32 mg/g in control group). Therefo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37263
更新日期:2016-11-23 00:00:00
abstract::Adiponectin regulates glucose and lipid metabolism, acting against metabolic syndrome and atherosclerosis. Accumulating evidence suggest that adiponectin acts on the brain including hypothalamic arcuate nucleus (ARC), where proopiomelanocortin (POMC) neurons play key roles in feeding regulation. Several studies have e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30796
更新日期:2016-08-09 00:00:00
abstract::This study was performed to evaluate clinical characteristics of branch retinal vein occlusion (BRVO) patients with increased retinal haemorrhage during macular oedema (MO) treatment. Patients were divided into increased and non-increased retinal haemorrhage groups. The former group was sub-divided based on the degree...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67395-7
更新日期:2020-06-24 00:00:00
abstract::Giant magnetoresistive (GMR) biosensors consisting of many rectangular stripes are being developed for high sensitivity medical diagnostics of diseases at early stages, but many aspects of the sensing mechanism remain to be clarified. Using e-beam patterned masks on the sensors, we showed that the magnetic nanoparticl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep18692
更新日期:2016-01-05 00:00:00
abstract::Parasitic lice (order Phthiraptera) infest birds and mammals. The typical animal mitochondrial (mt) genome organization, which consists of a single chromosome with 37 genes, was found in chewing lice in the suborders Amblycera and Ischnocera. The sucking lice (suborder Anoplura) known, however, have fragmented mt geno...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17389
更新日期:2015-11-30 00:00:00
abstract::The combustion of candles exhibits a variety of dynamical behaviors. Binding several candles together will result in flickering of candle flames, which is generally described as a nonlinear oscillator. The impact on the frequency of the flame by several factors, such as the arrangement, the number and the asymmetry of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36754-w
更新日期:2019-01-23 00:00:00
abstract::Fuel substrate supply and oxidative phosphorylation are key determinants of muscle performance. Numerous studies of mammalian mitochondria are carried out (i) with substrate supply that limits electron flow, and (ii) far below physiological temperature. To analyze potentially implicated biases, we studied mitochondria...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02789-8
更新日期:2017-06-06 00:00:00
abstract::Studies have assessed individual components of a western diet, but no study has assessed the long-term, cumulative effects of a western diet on aging and Alzheimer's disease (AD). Therefore, we have formulated the first western-style diet that mimics the fat, carbohydrate, protein, vitamin and mineral levels of wester...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21568
更新日期:2016-02-18 00:00:00
abstract::Electrochemical biosensors have been studied intensively for several decades. Numerous sensing concepts and related interface architectures have been developed. However, all such architectures suffer a trade-off: simple architectures favour usability, whereas complex architectures favour better performance. To overcom...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04169
更新日期:2014-02-25 00:00:00
abstract::Molecular chaperones assist proteins in achieving a functional structure and prevent them from misfolding into aggregates, including disease-associated deposits. The BRICHOS domain from familial dementia associated protein Bri2 (or ITM2B) probably chaperones its specific proprotein region with high β-sheet propensity ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66718-y
更新日期:2020-06-17 00:00:00
abstract::The use of agrochemicals in vegetable production could influence the selection for insecticide resistance in malaria vectors. Unfortunately, there is a dearth of information on the potential contribution of agrochemicals to insecticide resistance in Anopheles mosquitoes breeding on vegetable farms in southern Benin. A...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-63086-5
更新日期:2020-04-14 00:00:00
abstract::Dolphin morbillivirus (DMV) has been responsible for several outbreaks of systemic infection and has resulted in cetacean strandings in the Mediterranean. In August-October 2016, seven striped dolphins (Stenella coeruleoalba) stranded on the Sicilian coastline (Italy) tested positive for DMV. Tissue samples from brain...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46096-w
更新日期:2019-07-05 00:00:00
abstract::The S385Y/D469T/R520Q variant of E. coli transketolase was evolved previously with three successive smart libraries, each guided by different structural, bioinformatical or computational methods. Substrate-walking progressively shifted the target acceptor substrate from phosphorylated aldehydes, towards a non-phosphor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35716
更新日期:2016-10-21 00:00:00
abstract::Decarboxylation of fatty acids over non-noble metal catalysts without added hydrogen was studied. Ni/C catalysts were prepared and exhibited excellent activity and maintenance for decarboxylation. Thereafter, the effects of nickel loading, catalyst loading, temperature, and carbon number on the decarboxylation of fatt...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27820
更新日期:2016-06-13 00:00:00
abstract::One of the hallmarks of cancers is their ability to develop resistance against therapeutic agents. Therefore, developing effective in vitro strategies to identify drug resistance remains of paramount importance for successful treatment. One of the ways cancer cells achieve drug resistance is through the expression of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56273-6
更新日期:2019-12-27 00:00:00
abstract::Crops have different strategies to acquire poorly-available soil phosphorus (P) which are dependent on their architectural, morphological, and physiological root traits, but their capacity to enhance P acquisition varies with the type of fertilizer applied. The objective of this study was to examine how P-acquisition ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51204-x
更新日期:2019-10-16 00:00:00
abstract::The transition metal dichagenides and their metallic 1T structure are attracting contemporary attentions for applications in high-performance devices because their peculiar optical and electrical properties. The single and few layers 1T structure is generally obtained by mechanical or chemical exfoliation. This work p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45608
更新日期:2017-04-13 00:00:00
abstract::We propose a scheme of designing polygonal rotator with homogenous materials by using linear coordinate transformation. Our strategy is available for both time-harmonic electromagnetic field case and static field case. In particular, we found that only one anisotropic material is needed in static field case, and the d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51637-4
更新日期:2019-10-22 00:00:00
abstract::This study aimed to investigate the possible association between diabetes susceptibility gene transcription factor 7-like 2 (TCF7L2) and gestational diabetes mellitus (GDM) in a Chinese Han population. A total of 556 GDM patients and 500 Non-GDM were included. Eighteen single nucleotide polymorphisms (SNPs) were evalu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30686
更新日期:2016-07-28 00:00:00
abstract::Huntington's disease (HD) is caused by CAG repeat expansion in the huntingtin gene. The expanded polyglutamine (polyQ) repeat of the encoded protein leads to protein misfolding and aggregation, resulting in increased neuronal cell death. DNAJ co-chaperones play a crucial role in transferring misfolded/unfolded protein...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65046-5
更新日期:2020-05-18 00:00:00
abstract::Major depressive disorder (MDD) is a complex condition with unclear pathophysiology. Molecular disruptions within limbic brain regions and the periphery contribute to depression symptomatology and a more complete understanding the diversity of molecular changes that occur in these tissues may guide the development of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-75114-5
更新日期:2020-10-22 00:00:00
abstract::In this work, we report our study of protein expression in rat peri-infarct tissue, 48 h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12030
更新日期:2015-07-08 00:00:00
abstract::The anisotropic transport of thermal neutron in heterogeneous porous media is of great research interests in many fields. In this paper, it is the first time that a new model based on micron X-ray computed tomography (CT) has been proposed to simultaneously consider both the separation of matrix and pore and the distr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27488
更新日期:2016-06-08 00:00:00
abstract::Exercise physiology is different in males and females. Females are poorly studied due to the complexity of the estrous cycle and this bias has created an exercise sex gap. Here, we evaluated the impact of sexual dimorphism and of the estrous cycle on muscle strength and running power of C57BL/6 mice. Like men, male mi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29050-0
更新日期:2018-07-16 00:00:00