Axial plane optical microscopy.

Abstract:

:We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Li T,Ota S,Kim J,Wong ZJ,Wang Y,Yin X,Zhang X

doi

10.1038/srep07253

subject

Has Abstract

pub_date

2014-12-01 00:00:00

pages

7253

issn

2045-2322

pii

srep07253

journal_volume

4

pub_type

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