Cardiac endothelial cell-derived exosomes induce specific regulatory B cells.

Abstract:

:The mechanism of immune tolerance is to be further understood. The present study aims to investigate the role of the Cardiac endothelial cell (CEC)-derived exosomes in the induction of regulatory B cells. In this study, CECs were isolated from the mouse heart. Exosomes were purified from the culture supernatant of the primary endothelial cells. The suppressor functions of the regulatory B cells were determined by flow cytometry. The results showed that the CEC-derived exosomes carried integrin αvβ6. Exposure to lipopolysaccharide (LPS) induced B cells to express the latent transforming growth factor (TGF)-β, the latter was converted to the active form, TGF-β, by the exosome-derived αvβ6. The B cells released TGF-β in response to re-exposure to the exosomes in the culture, which suppressed the effector T cell proliferation. We conclude that CEC-derived exosomes have the capacity to induce B cells with immune suppressor functions.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Song J,Chen X,Wang M,Xing Y,Zheng Z,Hu S

doi

10.1038/srep07583

subject

Has Abstract

pub_date

2014-12-23 00:00:00

pages

7583

issn

2045-2322

pii

srep07583

journal_volume

4

pub_type

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