Abstract：
:Gold standard quantitative nucleic acid tests for diagnosis of viral diseases are currently limited to implementation in laboratories outside of the clinic. An instrument for conducting nucleic acid testing at the point-of-care (POC) that is easily operable by the clinician would reduce the required number of visits to the clinic and improve patient retention for proper treatment. Here we present a droplet magnetofluidic (DM) platform, which leverages functionalized magnetic particles to miniaturize and automate laboratory assays for use in the clinic at the POC. Our novel thermoformed disposable cartridge coupled to a portable multiaxial magnetofluidic instrument enables real-time PCR assays for quantitative and sensitive detection of nucleic acids from crude biosamples. Instead of laborious benchtop sample purification techniques followed by elution and spiking into PCR buffer, the user simply injects the biosample of interest into a cartridge with magnetic particles and loads the cartridge into the instrument. We demonstrate the utility of our platform with hepatitis C virus (HCV) RNA viral load quantitation from blood serum in approximately 1 hour. Clinical serum samples (n = 18) were directly processed on cartridges with no false positives and a limit of detection of 45 IU per 10 µl sample injection.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Shin DJ,Trick AY,Hsieh YH,Thomas DL,Wang THdoi
10.1038/s41598-018-28124-3subject
Has Abstractpub_date
2018-06-28 00:00:00pages
9793issue
1issn
2045-2322pii
10.1038/s41598-018-28124-3journal_volume
8pub_type
杂志文章abstract：:We evaluated the in-vitro effect of potassium on CD4+ T cells and the role of urinary potassium as a potential biomarker of disease activity in patients with ulcerative colitis (UC). This prospective observational cohort study included healthy controls (n = 18) and UC patients [n = 30, median age: 40 (IQR: 28-46) year...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18046-x
更新日期：2017-12-22 00:00:00
abstract：:E-mail has become the main carrier of spreading malicious software and been widely used for phishing, even high-level persistent threats. The e-mail accounts with high social reputation are primary targets to be attacked and utilized by attackers, suffering a lot of probing attacks for a long time. In this paper, in o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63191-5
更新日期：2020-04-29 00:00:00
abstract：:We propose an internal (on-chip) Wheatstone bridge configuration to evaluate the efficiency of near-field transducers (NFT) as used in heat-assisted magnetic recording (HAMR). The electric field enhancement between the transducer and the image plane is monitored by measuring the resistance of metal electrodes composin...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24423
更新日期：2016-04-14 00:00:00
abstract：:Grain boundary (GB) oxidation of proton-irradiated 304 nuclear grade stainless steel in primary water of pressurized water reactor was investigated. The investigation was conducted by studying microstructure of the oxide and oxide precursor formed at GB on an "atom-by-atom" basis by a combination of atom-probe tomogra...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80600-x
更新日期：2021-01-14 00:00:00
abstract：:Interleukin (IL)-27, a member of the IL-12 cytokine family, plays an important and diverse role in the function of the immune system. We have previously demonstrated that IL-27 is an anti-viral cytokine which inhibits HIV-1, HIV-2, Influenza virus and herpes simplex virus infection, and enhances the potential of react...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43441
更新日期：2017-02-27 00:00:00
abstract：:The individual impact of North Atlantic and Pacific Ocean Western Boundary Currents (OWBCs) on the tropospheric circulation has recently been studied in depth. However, their simultaneous role in shaping the hemisphere-scale wintertime troposphere/stratosphere-coupled circulation and its variability have not been cons...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39392-y
更新日期：2019-02-28 00:00:00
abstract：:When coming to identify new 2D materials, our intuition would suggest us to look from layered instead of 3D materials. However, since graphite can be hypothetically derived from diamond by stretching it along its [111] axis, many 3D materials can also potentially be explored as new candidates for 2D materials. Using a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24660
更新日期：2016-04-19 00:00:00
abstract：:Immobilized preys are routinely used in agro-ecological exposure studies to quantify predation of pests under field conditions, but this method has not been validated. Our purpose was to determine the validity of using immobilized adults of the major rice pest Nilaparvata lugens, brown plant hopper (BPH), as sentinels...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42210
更新日期：2017-02-17 00:00:00
abstract：:As mammalian herbivores feed, they often encounter noxious insects on plants. It is unknown how they handle such insects. We experimentally examined the behavioural responses of goats to the noxious spring-webworm (Ocnogyna loewii), and manipulated their sensory perception to reveal the process of insect detection. Go...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14940-6
更新日期：2017-11-01 00:00:00
abstract：:The COVID-19 pandemic has exceeded over sixty-five million cases globally. Different approaches are followed to mitigate its impact and reduce its spreading in different countries, but limiting mobility and exposure have been de-facto precautions to reduce transmission. However, a full lockdown cannot be sustained for...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78779-0
更新日期：2020-12-10 00:00:00
abstract：:We present a new class of TiBw/Ti6Al4V composites with a network reinforcement architecture that exhibits a significant creep resistance compared to monolithic Ti6Al4V alloys. Creep tests performed at temperatures between 773 K and 923 K and stress range of 100 MPa-300 MPa indicate both a significant improvement of th...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep40823
更新日期：2017-01-17 00:00:00
abstract：:We study the dynamics of an ultrafast single photon pulse in a one-dimensional waveguide two-point coupled with a Jaynes-Cummings system. We find that for any single photon input the transmissivity depends periodically on the separation between the two coupling points. For a pulse containing many plane wave components...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33867
更新日期：2016-09-22 00:00:00
abstract：:Incompatible patterns viewed by each of the two eyes can provoke binocular rivalry, a competition of perception. Levelt's first law predicts that a highly visible stimulus will predominate over a less visible stimulus during binocular rivalry. In a behavioural study, we made a counterintuitive observation: high visibi...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/s41598-018-32703-9
更新日期：2018-09-26 00:00:00
abstract：:Stress-induced viscous flow is the characteristic of atomic movements during plastic deformation of metallic glasses in the absence of substantial temperature increase, which suggests that stress state plays an important role in mechanically induced crystallization in a metallic glass. However, it is poorly understood...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep19358
更新日期：2016-01-13 00:00:00
abstract：:Cellular interactions can be modeled as complex dynamical systems represented by weighted graphs. The functionality of such networks, including measures of robustness, reliability, performance, and efficiency, are intrinsically tied to the topology and geometry of the underlying graph. Utilizing recently proposed geom...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12323
更新日期：2015-07-14 00:00:00
abstract：:Type-II Diabetes Mellitus (T2DM) is one of the fastest growing public health issues of modern times, consuming 12% of worldwide health budgets and affecting an estimated 400 million people. A key pathological trait associated with this disease is the failure of normal glucose-stimulated insulin secretion (GSIS) from p...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39681-6
更新日期：2019-02-27 00:00:00
abstract：:People of north-eastern states of India consume raw areca-nut (RAN) and lime which could lead to oral, esophageal and gastric cancers. However, the incidence of these cancers are significantly lesser in those who consume pieces of Potentilla fulgens root along with RAN. Since evaluation of anticancer role, if any, of ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53747-5
更新日期：2019-11-18 00:00:00
abstract：:The epithelial-mesenchymal plasticity, in tight association with stemness, contributes to the mammary gland homeostasis, evolution of early neoplastic lesions and cancer dissemination. Focused on cell surfaceome, we used mouse models of pre-neoplastic mammary epithelial and cancer stem cells to reveal the connection b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67827-4
更新日期：2020-07-09 00:00:00
abstract：:Niemann-Pick type C1 (NPC1) disease is a rare autosomal recessive, neurodegenerative lysosomal storage disorder, which presents with a range of clinical phenotypes and hence diagnosis remains a challenge. In view of these difficulties, the search for a novel, NPC1-specific biomarker (or set of biomarkers) is a topic o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06264-2
更新日期：2017-07-24 00:00:00
abstract：:Severe sepsis or septic shock is one of the rising causes for mortality worldwide representing nearly 10% of intensive care unit admissions. Susceptibility to sepsis is identified to be mediated by innate pattern recognition receptors and responsive signaling pathways of the host. The c-Jun N-terminal Kinase (JNK)-med...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07214
更新日期：2014-11-27 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-60918-2
更新日期：2020-02-27 00:00:00
abstract：:Nonalcoholic steatohepatitis (NASH) is a hepatic phenotype of the metabolic syndrome, and increases the risk of cirrhosis and hepatocellular carcinoma (HCC). Although increasing evidence points to the therapeutic implications of certain types of anti-diabetic agents in NASH, it remains to be elucidated whether their e...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57935-6
更新日期：2020-01-22 00:00:00
abstract：:STIM1 and Orai1 are the main components of a widely conserved Calcium influx pathway known as store-operated calcium entry (SOCE). STIM1 is a calcium sensor, which oligomerizes and activates Orai channels when calcium levels drop inside the endoplasmic reticulum (ER). The series of molecular rearrangements that STIM1 ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29634
更新日期：2016-07-27 00:00:00
abstract：:Symblepharon due to chemical burns affects ocular surface health, and there are currently no satisfactory treatments. To improve our understanding of symblepharon, an appropriate animal model is urgently needed. We established a rabbit model of superior conjunctival sac alkaline burn to evaluate symblepharon severity....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50286-x
更新日期：2019-09-25 00:00:00
abstract：:The goal of this study was to evaluate if isolated sarcomeres and half-sarcomeres produce a long-lasting increase in force after a stretch is imposed during activation. Single and half-sarcomeres were isolated from myofibrils using micro-needles, which were also used for force measurements. After full force developmen...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02320
更新日期：2013-01-01 00:00:00
abstract：:Phase estimation represents a crucial challenge in many fields of Physics, ranging from Quantum Metrology to Quantum Information Processing. This task is usually pursued by means of interferometric schemes, in which the choice of the input states and of the detection apparatus is aimed at minimizing the uncertainty in...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep26025
更新日期：2016-05-18 00:00:00
abstract：:Exposure to an adverse early-life environment leads to long-term health problems, many of which are recapitulated in subsequent generations. The female reproductive tract is particularly sensitive to early-life influences, and plays a pivotal role in programming the conceptus. We examine the influence of suboptimal gr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep16175
更新日期：2015-11-03 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-60509-1
更新日期：2020-02-20 00:00:00
abstract：:Pharmacotherapy provision based on Nicotine Metabolite Ratio (NMR) status (slow/normal metabolism) may improve smoking cessation rates. However, it is unclear whether NMR status is consistent across patient characteristics and current treatment choice. Data come from 1,826 participants attending Stop Smoking Services ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17994-8
更新日期：2017-12-14 00:00:00
abstract：:The ability to measure biomolecular dynamics within cells and tissues is very important to understand fundamental physiological processes including cell adhesion, signalling, movement, division or metabolism. Usually, such information is obtained using particle tracking methods or single point fluctuation spectroscopy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07048
更新日期：2014-11-14 00:00:00
