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TGF-β1 promotes cell barrier function upon maturation of corneal endothelial cells.

Abstract：

:Human corneal endothelial cells (HCECs) easily become fibroblastic-like when cultured, rendering them unsuitable for tissue engineering of the cornea. Transforming growth factor β (TGF-β) could be a key factor in this phenomenon; however, TGF-β is also known to maintain the endothelium in a quiescent state in vivo. This work aimed to compare the effects of TGF-β1 on the phenotype of HCECs during the proliferation and maturation phases. Our results show that addition of TGF-β1 during the active proliferation phase produced fibroblastic HCECs and loss of the cell junction markers ZO-1 and n-cadherin, independent from the presence of epidermal growth factor (EGF). By contrast, addition of TGF-β1 in maturation media containing few mitogens led to an endothelial phenotype and functional cell junctions as HCECs developed a high trans-endothelial resistance. Furthermore, addition of AG-1478, an epithelial growth factor receptor inhibitor, enhanced the gain of the endothelial phenotype and cell barrier function. Overall, these results show that TGF-β1 can be used to promote the formation of a typical leaky endothelial barrier during the maturation phase of cultured HCECs. A two-phase culture of HCECs using distinct proliferation and maturation media could also be key for developing ideal HCEC culture conditions.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Beaulieu Leclerc V,Roy O,Santerre K,Proulx S

doi

10.1038/s41598-018-22821-9

subject

Has Abstract

pub_date

2018-03-13 00:00:00

pages

4438

issue

1

issn

2045-2322

pii

10.1038/s41598-018-22821-9

journal_volume

8

pub_type

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