Abstract:
:Somatic mutations in NRAS, PTEN and AKT1 genes are rarely (~1%) reported in primary NSCLC, but their role in carcinogenesis have been proven. Therefore, we assessed the frequency of them in 145 FFPE tissue samples from CNS metastases of NSCLC using the real-time PCR technique. We identified four (two NRAS and single AKT1 and PTEN) mutations in CNS metastases of NSCLC. All mutations were observed in current male smokers (4% out of the male group; 4/100 and 4.25% out of smokers; 4/94). Three mutations have been detected in patients with SqCC (10.3% out of SqCC patients; 3/29), and only one mutation in the NRAS gene-in a patient with adenocarcinoma (1.25% out of AC patients; 1/80). The examined genes were mutually exclusive in terms of molecular background in KRAS; EGFR; DDR2; PIK3CA; HER2 and MEK1 genes that were evaluated in our previous studies. The OS of the patients who harbored NRAS, AKT1 and PTEN mutations was 10.1, 12.1, 7.3 and 4 months, respectively (vs 13.5 months of the studied group). Our results suggest that the presence of NRAS, PTEN and AKT1 gene mutations may have an influence on the occurrence of CNS metastases in patients with SqCC.
journal_name
Brain Tumor Patholjournal_title
Brain tumor pathologyauthors
Nicoś M,Krawczyk P,Jarosz B,Sawicki M,Trojanowski T,Milanowski Jdoi
10.1007/s10014-016-0276-2subject
Has Abstractpub_date
2017-01-01 00:00:00pages
36-41issue
1eissn
1433-7398issn
1861-387Xpii
10.1007/s10014-016-0276-2journal_volume
34pub_type
杂志文章abstract::Candidate genes involved in metastasis to the brain require investigation. In the present study, the adenomatous polyposis coli (APC) gene was analyzed in a set of human brain metastases. Gross deletions of the APC gene were tested by polymerase chain reaction/loss of heterozygosity (LOH) using the restriction fragmen...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-011-0030-8
更新日期:2011-07-01 00:00:00
abstract::Diffuse leptomeningeal glioneuronal tumor (DL-GNT) is a newly introduced tumor entity of uncertain prognosis characterised by a primary diffuse leptomeningeal growth pattern, oligodendroglial-like morphology and dual glial/neuronal differentiation. Predominantly occurring in children, these tumors present as chronic m...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-018-0325-0
更新日期:2018-07-01 00:00:00
abstract::Meningioma is the most common intracranial tumor, arising from arachnoid cells of the meninges. Monosomy 22 and inactivating mutations of NF2 are well-known genetic alterations of meningiomas. More recently, mutations in TRAF7, AKT1, KLF4, SMO, and PIK3CA were identified by next-generation sequencing. We here reviewed...
journal_title:Brain tumor pathology
pub_type: 杂志文章,评审
doi:10.1007/s10014-016-0271-7
更新日期:2016-10-01 00:00:00
abstract::Despite recent advances in the diagnosis and treatment of glioblastomas, patient outcomes for these highly malignant tumors remain poor. Research into the molecular pathology of glioblastoma has uncovered various genetic changes that contribute to malignancy. Some of the identified molecular markers--such as loss of h...
journal_title:Brain tumor pathology
pub_type: 杂志文章,评审
doi:10.1007/s10014-011-0038-0
更新日期:2011-07-01 00:00:00
abstract::The present retrospective data analysis was performed to determine whether intraoperative pathological diagnosis (IOD) using frozen section (FS) could clearly distinguish high-grade glioma from WHO grade II gliomas. IOD was retrospectively compared to the pathological diagnosis using permanent paraffin sections (PS) o...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-014-0177-1
更新日期:2014-07-01 00:00:00
abstract::Detection of mutations in the isocitrate dehydrogenase 1 (IDH1) gene is useful for accurate diagnosis of lower grade gliomas, as described in the 2016 World Health Organization classification of tumors of the central nervous system. Conventional analysis tools, including Sanger DNA sequencing and immunohistochemistry,...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-018-0310-7
更新日期:2018-04-01 00:00:00
abstract::A 55-year old female was referred to the Department of Neurosurgery, Kitasato Hospital, because of a hearing impairment. Neuroimaging revealed a typical meningioma attached to the falx in the right frontal region. During surgery, an encapsulated, circumscribed, reddish-gray, slightly hard tumor attached to the falx wa...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-012-0091-3
更新日期:2013-01-01 00:00:00
abstract::Ollier disease (OD) and Maffucci syndrome are rare conditions due to a post-zygotic somatic mutation that results in mosaicism. In addition to enchondromas and hemangiomas, some of these patients also develop other unrelated tumors, such as gliomas, that harbor IDH mutations, suggesting that an IDH mutation is a commo...
journal_title:Brain tumor pathology
pub_type: 杂志文章,评审
doi:10.1007/s10014-018-0327-y
更新日期:2018-10-01 00:00:00
abstract::Although antiangiogenic treatment for malignant glioma using bevacizumab in combination with irinotecan chemotherapy has a promising effect on survival, the high incidence of increasing infiltrative tumors can be a problem in resistance to antiangiogenic therapy. In this study, we detected failure of bevacizumab treat...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-010-0271-y
更新日期:2010-10-01 00:00:00
abstract::The IDH-mutant and 1p/19q co-deletion (1p19q codel) provides significant diagnostic and prognostic value in lower-grade gliomas. As ATRX mutation and 1p19q codel are mutually exclusive, ATRX immunohistochemistry (IHC) may substitute for 1p19q codel, but this has not been comprehensively examined. In the current study,...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-018-0312-5
更新日期:2018-04-01 00:00:00
abstract::We describe a new case of isolated Langerhans cell histiocytosis (LCH) of the hypothalamus. A 53-year-old female patient presented with polydipsia, headache, anorexia, and fatigue. Neurological imaging revealed a mass projecting from the hypothalamus into the third ventricle. Gross total removal of the tumor was perfo...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/BF02484517
更新日期:2004-01-01 00:00:00
abstract::L-type amino acid transporter 1 (LAT1), a neutral amino acid transport agent, is essential for the transport of large neutral amino acids. LAT1 also corresponds to tumor-associated gene-1 (TA1), an oncofetal antigen that is expressed primarily in fetal tissues and cancer cells such as glioma cells. We have investigate...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-005-0188-z
更新日期:2005-01-01 00:00:00
abstract::Congenital malignant gliomas are rare brain tumors about which few reports have been published. We present the clinical course and genetic alterations in an infant with a congenital malignant glioma detected incidentally by ultrasonography at 36 weeks. The tumor occupied the right temporoparietal region, extended to t...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-011-0071-z
更新日期:2012-04-01 00:00:00
abstract::Recent reports have suggested an important clinical role for hypermethylation of the O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter in patients with glioblastomas. Whether MGMT protein expression is correlated with promoter hypermethylation and patient outcomes, however, has not been elucidated. Here we desc...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-010-0004-2
更新日期:2011-02-01 00:00:00
abstract::To identify differentially expressed genes involved in rat C6 glioma cell differentiation induced by cyclic AMP, we adopted mRNA fingerprinting using arbitrarily primed polymerase chain reaction (PCR) (RAP). Four complementary DNA (cDNA) fragments differentially expressed during differentiation were isolated, and they...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/BF02478880
更新日期:1997-01-01 00:00:00
abstract::The role of intraoperative pathological diagnosis for central nervous system (CNS) tumors is crucial for neurosurgery when determining the surgical procedure. Especially, treatment of carmustine (BCNU) wafers requires a conclusive diagnosis of high-grade glioma proven by intraoperative diagnosis. Recently, we demonstr...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-015-0238-0
更新日期:2016-01-01 00:00:00
abstract::To elucidate the expression of the MDR1 gene products P-glycoprotein (Pgp) in endothelial cells of newly formed blood microvessels in brain tumors, 30 brain tumors were examined by immunohistochemistry using an anti-Pgp monoclonal antibody, JSB-1. Positive reactions for JSB-1 were detected in endothelial cells in newl...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/BF02478898
更新日期:1999-01-01 00:00:00
abstract::Diffuse gliomas are highly infiltrative intracranial tumors, but there are few useful markers for detecting infiltrating glioma cells in the surrounding brain tissue. Doublecortin (DCX) is a microtubule-associated protein (MAP) that plays a crucial role in neuroblast migration. It was recently demonstrated that DCX is...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-007-0225-1
更新日期:2008-01-01 00:00:00
abstract::Recent studies on gliomas have shown frequent alterations in the alpha-thalassemia/mental retardation syndrome X-linked gene (ATRX). This study was designed to determine whether ATRX status correlates with uptake of 11C-methionine in WHO grades II and III gliomas. Sixty-two patients underwent 11C-methionine positron e...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-017-0280-1
更新日期:2017-01-01 00:00:00
abstract::Investigations on the NAB2-STAT6 fusion gene in solitary fibrous tumors (SFTs) and hemangiopericytomas (HPCs) have increased since its discovery in 2013. Although several SFTs reported without NAB2-STAT6 fusion gene analysis, we reviewed 546 SFTs/HPCs with NAB2-STAT6 fusion gene analysis in this study and investigated...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-016-0264-6
更新日期:2016-07-01 00:00:00
abstract::A 37-year-old man, a hepatitis B virus carrier due to mother-to-child transmission, had a medical examination in September 2008 in nearby hospitals due to anorexia and weight loss. He was transported to our hospital because computed tomography (CT) detected intracranial lesions, and he had a positive human immunodefic...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-010-0001-5
更新日期:2011-02-01 00:00:00
abstract::Epithelioid glioblastomas (E-GBMs) are rare, highly aggressive tumors consisting of closely packed tumor cells with smooth, round cell borders and abundant eosinophilic cytoplasm. They tend to affect younger patients compared with conventional GBM. BRAF V600E mutation is characteristically found in approximately 50% o...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-015-0239-z
更新日期:2016-01-01 00:00:00
abstract::Glioblastoma with a primitive neuronal component (GBM-PN) was renamed from glioblastoma with primitive neuroectodermal tumor-like component (GBM-PNET) in the new WHO classification of tumors of the central nervous system in 2016. GBM-PN is a rare variant of glioblastoma. There were not so many publications on the inve...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-019-00334-1
更新日期:2019-07-01 00:00:00
abstract::The objective of this study is to clarify clinical significance of the H3F3A K27M mutation (H3K27M) and analyze the correlation between H3K27M, H3K27me3 status, and EZH2 expression and prognosis in spinal cord gliomas. Patients with spinal cord diffuse glioma regardless of World Health Organization (WHO) grade underwe...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-020-00369-9
更新日期:2020-07-01 00:00:00
abstract::There is now compelling evidence that gliomas harbor a small population of cells, termed glioma-initiating cells (GICs), characterized by their ability to undergo self-renewal and initiate tumorigenesis. The development of therapeutic strategies targeted toward GIC signaling may improve the treatment of malignant glio...
journal_title:Brain tumor pathology
pub_type: 杂志文章,评审
doi:10.1007/s10014-010-0011-3
更新日期:2011-02-01 00:00:00
abstract::The clinical and histological characteristics of oligodendroglioma and oligoastrocytoma were investigated in patients, mainly adults with supratentorial tumors, who were treated with surgery and radiotherapy, and with chemotherapy for recurrent, anaplastic tumors, or both. The median survival time was 13.2 years for o...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/BF02478866
更新日期:1997-01-01 00:00:00
abstract::Chordomas and chondrosarcomas are two major malignant bone neoplasms located at the skull base. These tumors are rarely metastatic, but can be locally invasive and resistant to conventional chemotherapies and radiotherapies. Accordingly, therapeutic approaches for the treatment of these tumors can be difficult. Additi...
journal_title:Brain tumor pathology
pub_type: 杂志文章,评审
doi:10.1007/s10014-017-0283-y
更新日期:2017-04-01 00:00:00
abstract::Dysembryoplastic neuroepithelial tumor (DNT) is a relatively new nosologic entity. First described in 1988, it is now included in the "neuronal and mixed neuronal-glial tumours" category in the revised 2000 World Health Organization (WHO) Classification of Tumours of the Nervous System. The collective experience of mo...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/BF02478934
更新日期:2002-01-01 00:00:00
abstract::With the use of RT-PCR (reverse transcriptase-polymerase chain reaction), Northern blot analysis, and Western blot analysis, seven primary brain lymphomas were examined for the state of the MMACI tumor suppressor gene. Nucleotide analysis of RT-PCR clones revealed no abnormality in the MMAC1 coding sequence in each ca...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/BF02479427
更新日期:2001-01-01 00:00:00
abstract::Accumulating evidence suggests that tissue hypoxia and apoptosis play important roles in the malignant progression of brain tumors. We investigated the relationship of 14-3-3zeta (an apoptosis-related protein), HIF-1α, and VEGF immunohistochemistry, and evaluated the prognostic value of their expression in human brain...
journal_title:Brain tumor pathology
pub_type: 杂志文章
doi:10.1007/s10014-013-0135-3
更新日期:2014-01-01 00:00:00