Rapid immunocytochemistry based on alternating current electric field using squash smear preparation of central nervous system tumors.

Abstract:

:The role of intraoperative pathological diagnosis for central nervous system (CNS) tumors is crucial for neurosurgery when determining the surgical procedure. Especially, treatment of carmustine (BCNU) wafers requires a conclusive diagnosis of high-grade glioma proven by intraoperative diagnosis. Recently, we demonstrated the usefulness of rapid immunohistochemistry (R-IHC) that facilitates antigen-antibody reaction under alternative current (AC) electric field in the intraoperative diagnosis of CNS tumors; however, a higher proportion of water and lipid in the brain parenchyma sometimes leads to freezing artifacts, resulting in poor quality of frozen sections. On the other hand, squash smear preparation of CNS tumors for cytology does not affect the frozen artifacts, and the importance of smear preparation is now being re-recognized as being better than that of the tissue sections. In this study, we established the rapid immunocytochemistry (R-ICC) protocol for squash smears of CNS tumors using AC electric field that takes only 22 min, and demonstrated its usefulness for semi-quantitative Ki-67/MIB-1 labeling index and CD 20 by R-ICC for intraoperative diagnosis. R-ICC by AC electric field may become a substantial tool for compensating R-IHC and will be applied for broad antibodies in the future.

journal_name

Brain Tumor Pathol

journal_title

Brain tumor pathology

authors

Moriya J,Tanino MA,Takenami T,Endoh T,Urushido M,Kato Y,Wang L,Kimura T,Tsuda M,Nishihara H,Tanaka S,R-IHC Study Group.

doi

10.1007/s10014-015-0238-0

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

13-8

issue

1

eissn

1433-7398

issn

1861-387X

pii

10.1007/s10014-015-0238-0

journal_volume

33

pub_type

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