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Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module.

Abstract：

:Nanoparticle-mediated delivery of functional macromolecules is a promising method for treating a variety of human diseases. Among nanoparticles, cell-derived exosomes have recently been highlighted as a new therapeutic strategy for the in vivo delivery of nucleotides and chemical drugs. Here we describe a new tool for intracellular delivery of target proteins, named 'exosomes for protein loading via optically reversible protein-protein interactions' (EXPLORs). By integrating a reversible protein-protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and their function in recipient cells in vitro and in vivo. These results clearly indicate the potential of EXPLORs as a mechanism for the efficient intracellular transfer of protein-based therapeutics into recipient cells and tissues.

journal_name

Nat Commun

journal_title

Nature communications

authors

Yim N,Ryu SW,Choi K,Lee KR,Lee S,Choi H,Kim J,Shaker MR,Sun W,Park JH,Kim D,Heo WD,Choi C

doi

10.1038/ncomms12277

subject

Has Abstract

pub_date

2016-07-22 00:00:00

pages

12277

issn

2041-1723

pii

10.1038/ncomms12277

journal_volume

7

pub_type

杂志文章

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