Abstract:
:Continuous cycles of nucleocytoplasmic transport require disassembly of transport receptor/Ran-GTP complexes in the cytoplasm. A basic disassembly mechanism in all eukaryotes depends on soluble RanGAP and RanBP1. In vertebrates, a significant fraction of RanGAP1 stably interacts with the nucleoporin RanBP2 at a binding site that is flanked by FG-repeats and Ran-binding domains, and overlaps with RanBP2's SUMO E3 ligase region. Here, we show that the RanBP2/RanGAP1*SUMO1/Ubc9 complex functions as an autonomous disassembly machine with a preference for the export receptor Crm1. We describe three in vitro reconstituted disassembly intermediates, which show binding of a Crm1 export complex via two FG-repeat patches, cargo-release by RanBP2's Ran-binding domains and retention of free Crm1 at RanBP2 after Ran-GTP hydrolysis. Intriguingly, all intermediates are compatible with SUMO E3 ligase activity, suggesting that the RanBP2/RanGAP1*SUMO1/Ubc9 complex may link Crm1- and SUMO-dependent functions.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Ritterhoff T,Das H,Hofhaus G,Schröder RR,Flotho A,Melchior Fdoi
10.1038/ncomms11482subject
Has Abstractpub_date
2016-05-10 00:00:00pages
11482issn
2041-1723pii
ncomms11482journal_volume
7pub_type
杂志文章abstract::Interfacial reactions between electrode and electrolyte are critical, either beneficial or detrimental, for the performance of rechargeable batteries. The general approaches of controlling interfacial reactions are either applying a coating layer on cathode or modifying the electrolyte chemistry. Here we demonstrate a...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-11299-2
更新日期:2019-08-01 00:00:00
abstract::Phonons are the main source of relaxation in molecular nanomagnets, and different mechanisms have been proposed in order to explain the wealth of experimental findings. However, very limited experimental investigations on phonons in these systems have been performed so far, yielding no information about their dispersi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15475-7
更新日期:2020-04-09 00:00:00
abstract::Quantum computers promise to solve certain problems more efficiently than their digital counterparts. A major challenge towards practically useful quantum computing is characterizing and reducing the various errors that accumulate during an algorithm running on large-scale processors. Current characterization techniqu...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13068-7
更新日期:2019-11-25 00:00:00
abstract::Geranyl-CoA carboxylase (GCC) is essential for the growth of Pseudomonas organisms with geranic acid as the sole carbon source. GCC has the same domain organization and shares strong sequence conservation with the related biotin-dependent carboxylases 3-methylcrotonyl-CoA carboxylase (MCC) and propionyl-CoA carboxylas...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9986
更新日期:2015-11-23 00:00:00
abstract::The behavior of uranium in environments, ranging from those of natural systems responsible for the formation of uranium deposits to those of nuclear reactors providing 11% of the world's electricity, is governed by processes involving high-temperature aqueous solutions. It has been well documented that uranium is mobi...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03564-7
更新日期:2018-04-16 00:00:00
abstract::Extracellular matrix interactions have essential roles in normal physiology and many pathological processes. Although the importance of extracellular matrix interactions in metastasis is well documented, systematic approaches to identify their roles in distinct stages of tumorigenesis have not been described. Here we ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms2128
更新日期:2012-01-01 00:00:00
abstract::Synthetic genetic circuits allow us to modify the behavior of living cells. However, changes in environmental conditions and unforeseen interactions with the host cell can cause deviations from a desired function, resulting in the need for time-consuming reassembly to fix these issues. Here, we use a regulatory motif ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-15653-7
更新日期:2020-04-29 00:00:00
abstract::Maize rough dwarf disease (MRDD), caused by various species of the genus Fijivirus, threatens maize production worldwide. We previously identified a quantitative locus qMrdd1 conferring recessive resistance to one causal species, rice black-streaked dwarf virus (RBSDV). Here, we show that Rab GDP dissociation inhibito...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14372-3
更新日期:2020-01-24 00:00:00
abstract::Human endogenous retroviruses (HERV) form a substantial part of the human genome, but mostly remain transcriptionally silent under strict epigenetic regulation, yet can potentially be reactivated by malignant transformation or epigenetic therapies. Here, we evaluate the potential for T cell recognition of HERV element...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-19464-8
更新日期:2020-11-09 00:00:00
abstract::Congenital Zika syndrome (CZS) causes early brain development impairment by affecting neural progenitor cells (NPCs). Here, we analyze NPCs from three pairs of dizygotic twins discordant for CZS. We compare by RNA-Seq the NPCs derived from CZS-affected and CZS-unaffected twins. Prior to Zika virus (ZIKV) infection the...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02790-9
更新日期:2018-02-02 00:00:00
abstract::Much of the global annual mean temperature change over Quaternary glacial cycles can be attributed to slow ice sheet and greenhouse gas feedbacks, but analysis of the short-term response to orbital forcings has the potential to reveal key relationships in the climate system. In particular, obliquity and precession bot...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03800-0
更新日期:2018-04-10 00:00:00
abstract::Proximity-ligation methods such as Hi-C allow us to map physical DNA-DNA interactions along the genome, and reveal its organization into topologically associating domains (TADs). As the Hi-C data accumulate, computational methods were developed for identifying domain borders in multiple cell types and organisms. Here,...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-017-02386-3
更新日期:2017-12-21 00:00:00
abstract::We have developed a single-breath-hold photoacoustic computed tomography (SBH-PACT) system to reveal detailed angiographic structures in human breasts. SBH-PACT features a deep penetration depth (4 cm in vivo) with high spatial and temporal resolutions (255 µm in-plane resolution and a 10 Hz 2D frame rate). By scannin...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-04576-z
更新日期:2018-06-15 00:00:00
abstract::Metal halide perovskite semiconductors possess outstanding characteristics for optoelectronic applications including but not limited to photovoltaics. Low-dimensional and nanostructured motifs impart added functionality which can be exploited further. Moreover, wider cation composition tunability and tunable surface l...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10856-z
更新日期:2019-06-28 00:00:00
abstract::T cells expressing CD19-targeting chimeric antigen receptors (CARs) reveal high efficacy in the treatment of B cell malignancies. Here, we report that T cell receptor fusion constructs (TRuCs) comprising an antibody-based binding domain fused to T cell receptor (TCR) subunits can effectively reprogram an intact TCR co...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-10097-0
更新日期:2019-05-07 00:00:00
abstract::Conjugated polymeric molecules have been heralded as promising electrode materials for the next-generation energy-storage technologies owing to their chemical flexibility at the molecular level, environmental benefit, and cost advantage. However, before any practical implementation takes place, the low capacity, poor ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-02889-7
更新日期:2018-02-08 00:00:00
abstract::γδ T cells are considered to be innate-like lymphocytes that respond rapidly to stress without clonal selection and differentiation. Here we use next-generation sequencing to probe how this paradigm relates to human Vδ2neg T cells, implicated in responses to viral infection and cancer. The prevalent Vδ1 T cell recepto...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14760
更新日期:2017-03-01 00:00:00
abstract::Graphite dual-ion batteries represent a potential battery concept for large-scale stationary storage of electricity, especially when constructed free of lithium and other chemical elements with limited natural reserves. Owing to their non-rocking-chair operation mechanism, however, the practical deployment of graphite...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-06923-6
更新日期:2018-10-26 00:00:00
abstract::Identifying a bona fide population of cardiac stem cells (CSCs) is a critical step for developing cell-based therapies for heart failure patients. Previously, cardiac c-kit(+) cells were reported to be CSCs with a potential to become myocardial, endothelial and smooth muscle cells in vitro and after cardiac injury. He...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms9701
更新日期:2015-10-30 00:00:00
abstract::Chloride intracellular channel 4 (CLIC4) is a mammalian homologue of EXC-4 whose mutation is associated with cystic excretory canals in nematodes. Here we show that CLIC4-null mouse embryos exhibit impaired renal tubulogenesis. In both developing and developed kidneys, CLIC4 is specifically enriched in the proximal tu...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms10412
更新日期:2016-01-20 00:00:00
abstract::Denervation of skeletal muscles induces severe muscle atrophy, which is preceded by cellular alterations such as increased plasma membrane permeability, reduced resting membrane potential and accelerated protein catabolism. The factors that induce these changes remain unknown. Conversely, functional recovery following...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-14063-8
更新日期:2020-02-26 00:00:00
abstract::Streptomycetes are notable for their complex life cycle and production of most clinically important antibiotics. A key factor that controls entry into development and the onset of antibiotic production is the 68-residue protein, BldC. BldC is a putative DNA-binding protein related to MerR regulators, but lacks coiled-...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03576-3
更新日期:2018-03-19 00:00:00
abstract::Pladienolide, herboxidiene and spliceostatin have been identified as splicing modulators that target SF3B1 in the SF3b subcomplex. Here we report that PHF5A, another component of this subcomplex, is also targeted by these compounds. Mutations in PHF5A-Y36, SF3B1-K1071, SF3B1-R1074 and SF3B1-V1078 confer resistance to ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms15522
更新日期:2017-05-25 00:00:00
abstract::Transposable elements constitute a substantial portion of eukaryotic genomes and contribute to genomic variation, function, and evolution. Miniature inverted-repeat transposable elements (MITEs), as DNA transposons, are widely distributed in plant and animal genomes. Previous studies have suggested that retrotransposo...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms14651
更新日期:2017-03-03 00:00:00
abstract::Deregulation of mitochondrial network in terminally differentiated cells contributes to a broad spectrum of disorders. Methylmalonic acidemia (MMA) is one of the most common inherited metabolic disorders, due to deficiency of the mitochondrial methylmalonyl-coenzyme A mutase (MMUT). How MMUT deficiency triggers cell d...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-020-14729-8
更新日期:2020-02-20 00:00:00
abstract::Defects can greatly influence the properties of oxide materials; however, facile defect engineering of oxides at room temperature remains challenging. The generation of defects in oxides is difficult to control by conventional chemical reduction methods that usually require high temperatures and are time consuming. He...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-018-03765-0
更新日期:2018-04-03 00:00:00
abstract::To date, the vast preponderance of somatic variants observed in the cancer genome have been rare variants, and it is common in practice to encounter in a new tumor variants that have not been observed previously. Here we focus on probability estimation for encountering such hitherto unseen variants. We draw upon stati...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-13402-z
更新日期:2019-12-03 00:00:00
abstract::Coherence in light-matter interaction is a necessary ingredient if light is used to control the quantum state of a material system. Coherent effects are firmly associated with isolated systems kept at low temperature. The exceedingly fast dephasing in condensed matter environments, in particular at elevated temperatur...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3953
更新日期:2013-01-01 00:00:00
abstract::Τhe morphology, physiology and immunology, of solid tumors exhibit spatial heterogeneity which complicates our understanding of cancer progression and therapy response. Understanding spatial heterogeneity necessitates high resolution in vivo imaging of anatomical and pathophysiological tumor information. We introduce ...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/s41467-019-09081-5
更新日期:2019-03-13 00:00:00
abstract::Advances in chemistry and massively parallel detection underlie DNA-sequencing platforms that are poised for application in personalized medicine. In stark contrast, systematic generation of protein-level data lags well behind genomics in virtually every aspect: depth of coverage, throughput, ease of sample preparatio...
journal_title:Nature communications
pub_type: 杂志文章
doi:10.1038/ncomms3171
更新日期:2013-01-01 00:00:00