Abstract:
:Advances in chemistry and massively parallel detection underlie DNA-sequencing platforms that are poised for application in personalized medicine. In stark contrast, systematic generation of protein-level data lags well behind genomics in virtually every aspect: depth of coverage, throughput, ease of sample preparation and experimental time. Here, to bridge this gap, we develop an approach based on simple detergent lysis and single-enzyme digest, extreme, orthogonal separation of peptides and true nanoflow liquid chromatography-tandem mass spectrometry that provides high peak capacity and ionization efficiency. This automated, deep efficient peptide sequencing and quantification mass spectrometry platform provides genome-scale proteome coverage equivalent to RNA-seq ribosomal profiling and accurate quantification for multiplexed isotope labels. In a model of the embryonic to epiblast transition in murine stem cells, we unambiguously quantify 11,352 gene products that span 70% of Swiss-Prot and capture protein regulation across the full detectable range of high-throughput gene expression and protein translation.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Zhou F,Lu Y,Ficarro SB,Adelmant G,Jiang W,Luckey CJ,Marto JAdoi
10.1038/ncomms3171subject
Has Abstractpub_date
2013-01-01 00:00:00pages
2171issn
2041-1723pii
ncomms3171journal_volume
4pub_type
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