Abstract:
:Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 Å resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.
journal_name
Nat Communjournal_title
Nature communicationsauthors
Tan YZ,Rodrigues J,Keener JE,Zheng RB,Brunton R,Kloss B,Giacometti SI,Rosário AL,Zhang L,Niederweis M,Clarke OB,Lowary TL,Marty MT,Archer M,Potter CS,Carragher B,Mancia Fdoi
10.1038/s41467-020-17202-8subject
Has Abstractpub_date
2020-07-07 00:00:00pages
3396issue
1issn
2041-1723pii
10.1038/s41467-020-17202-8journal_volume
11pub_type
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