Artificial morphogen-mediated differentiation in synthetic protocells.

Abstract:

:The design and assembly of artificial protocell consortia displaying dynamical behaviours and systems-based properties are emerging challenges in bottom-up synthetic biology. Cellular processes such as morphogenesis and differentiation rely in part on reaction-diffusion gradients, and the ability to mimic rudimentary aspects of these non-equilibrium processes in communities of artificial cells could provide a step to life-like systems capable of complex spatiotemporal transformations. Here we expose acoustically formed arrays of initially identical coacervate micro-droplets to uni-directional or counter-directional reaction-diffusion gradients of artificial morphogens to induce morphological differentiation and spatial patterning in single populations of model protocells. Dynamic reconfiguration of the droplets in the morphogen gradients produces a diversity of membrane-bounded vesicles that are spontaneously segregated into multimodal populations with differentiated enzyme activities. Our results highlight the opportunities for constructing protocell arrays with graded structure and functionality and provide a step towards the development of artificial cell platforms capable of multiple operations.

journal_name

Nat Commun

journal_title

Nature communications

authors

Tian L,Li M,Patil AJ,Drinkwater BW,Mann S

doi

10.1038/s41467-019-11316-4

subject

Has Abstract

pub_date

2019-07-25 00:00:00

pages

3321

issue

1

issn

2041-1723

pii

10.1038/s41467-019-11316-4

journal_volume

10

pub_type

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