Abstract：
:Level of adenosine, an endogenous astrocyte-based neuromodulator, is primarily regulated by adenosine P1 receptors. This study assessed expression of adenosine P1 receptors, ADORA1 (adenosine A1 receptor) and ADORA2A (adenosine A2a receptor) and their association with glioma development and epilepsy in glioma patients. Expression of ADORA1/ADORA2A was assessed immunohistochemically in 65 surgically removed glioma tissue and 21 peri-tumor tissues and 8 cases of normal brain tissues obtained from hematoma patients with cerebral trauma. Immunofluorescence, Western blot, and qRT-PCR were also used to verify immunohistochemical data. Adenosine P1 receptor ADORA1 and ADORA2A proteins were localized in the cell membrane and cytoplasm and ADORA1/ADORA2A immunoreactivity was significantly stronger in glioma and peri-tumor tissues that contained infiltrating tumor cells than in normal brain tissues (p < 0.05). The World Health Organization (WHO) grade III gliomas expressed even higher level of ADORA1 and ADORA2A. Western blot and qRT-PCR confirmed immunohistochemical data. Moreover, higher levels of ADORA1 and ADORA2A expression occurred in high-grade gliomas, in which incidence of epilepsy were lower (p < 0.05). In contrast, a lower level of ADORA1/ADORA2A expression was found in peri-tumor tissues with tumor cell presence from patients with epilepsy compared to patients without epilepsy (p < 0.05). The data from the current study indicates that dysregulation in ADORA1/ADORA2A expression was associated with glioma development, whereas low level of ADORA1/ADORA2A expression could increase susceptibility of tumor-associated epilepsy.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Huang J,Chen MN,Du J,Liu H,He YJ,Li GL,Li SY,Liu WP,Long XYdoi
10.1007/s11064-016-1893-1subject
Has Abstractpub_date
2016-07-01 00:00:00pages
1774-83issue
7eissn
0364-3190issn
1573-6903pii
10.1007/s11064-016-1893-1journal_volume
41pub_type
杂志文章abstract：:The effects of chronic pentobarbital (PB) treatment on the binding characteristics of [3H]flunitrazepam (FLU) in rat brain were examined. Saline or sodium PB (500 micrograms/10 microliters/hr) was infused into the lateral cerebral ventricles of rats for 6 days using osmotic pumps. Immediately before withdrawal, there ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966726
更新日期：1994-01-01 00:00:00
abstract：:To clarify the molecular basis of the cytoprotective properties of immunophilin ligands (IPLs), the anti-apoptotic effects of IPLs were determined in human glioma U251 cells. GPI1046 and V10367, non-immunosuppressive IPLs (NI-IPLs), as well as FK506, an immunosuppressive IPL (I-IPL), had cytoprotective effects against...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000029565.92587.25
更新日期：2004-08-01 00:00:00
abstract：:We review some of the older literature concerning metabolic turnover of cholesterol in the nervous system. The overall picture is that incorporation of radioactive precursors into brain cholesterol is roughly proportional to the rate of myelination and that, once incorporated, radioactive cholesterol is relatively sta...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF02527711
更新日期：1996-04-01 00:00:00
abstract：:The exposure to hypobaric hypoxia increased lipid peroxidation as indicated by thiobarbituric acid-reactive substances [TBARS] in rat brain. Plasma lactate/pyruvate ratio was used as a marker of hypoxia. We compared the protective effect of alpha-tocopherol with the effect of L-carnitine or phosphocreatine. Rats pretr...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020339530924
更新日期：2002-09-01 00:00:00
abstract：:The present study examined the potential membrane retention of desipramine (DMI) following exposures of 293-hNET cells to DMI, and its effect on [3H]NE uptake. Incubation of cells with 500 nM DMI for 1 h or 1 day persistently inhibited the uptake of [3H]NE up to 7 days, despite daily repeated washing of cells with dru...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000013747.04964.46
更新日期：2004-02-01 00:00:00
abstract：:Exposure to Hg2+ below 10 micrometer destroys synaptosomal membrane-associated sialidase of bovine brain in situ. Inhibition by Cu2+ occurs only at relatively higher concentrations, and is demonstrable after the synaptosomal membrane preparation has been presaturated with Cu2+ . Pb2+ does not inhibit enzymatic activit...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964363
更新日期：1978-02-01 00:00:00
abstract：:Neuroglycan C (NGC) is a transmembrane-type chondroitin sulfate proteoglycan that promotes neurite outgrowth. To identify the ligand of NGC, we applied a detergent-solubilized membrane fraction of fetal rat brains to an NGC-immobilized affinity column. Several proteins were eluted from the column including an 18 kDa-b...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-010-0164-9
更新日期：2010-08-01 00:00:00
abstract：:The particulars regarding the amine precursor therapy for treatment of neuropsychiatric disorders and their neurochemical background are described historically. Furthermore, peculiar or artificial amino acids which are metabolized into the brain amine or their derivatives and promised their clinical application are al...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/BF02529142
更新日期：1996-02-01 00:00:00
abstract：:Parkinson's disease (PD) is a common progressive neurodegenerative disorder, for which at present no causal treatment is available. On the understanding that the causes of PD are mainly oxidative stress and mitochondrial dysfunction, antioxidants and other drugs are expected to be used. In the present study, we demons...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-009-0046-1
更新日期：2010-02-01 00:00:00
abstract：:Recently we have demonstrated that extracellular ATP acts as an excitatory neurotransmitter and enhances cell death in the presence of ferrous ions. By using a newly developed cis-parinaric acid fluorescence technique, we demonstrated that ATP, in a dose dependent manner, enhanced the increased membrane lipid peroxida...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF02527668
更新日期：1996-01-01 00:00:00
abstract：:A panel of mouse monoclonal antibodies to rat and human myelin-associated glycoprotein (MAG) was developed. Normal mice were unresponsive to rat MAG, and successful immunization with rat MAG was only achieved in autoimmune NZB mice. By contrast, all strains of mice were responsive to human MAG. The monoclonal antibodi...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00964654
更新日期：1985-04-01 00:00:00
abstract：:We previously showed that extracellular levels of N-acetylaspartate (NAA) increase when a medium with reduced NaCl concentration is perfused through a microdialysis probe, and proposed that NAA may be released during hypoosmotic swelling. Here, we demonstrate that this effect is due to hypoosmolarity of the perfusion ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1020778832745
更新日期：1998-08-01 00:00:00
abstract：:The changes in both the levels of some free amino acids and their metabolism in the rat brain during the first 24 hr of postnatal life were studied. The content of glutamic acid decreased for the first 2 hr; it remained at the lowest level for the next 4 hr, when it began to increase. The content of alanine decreased ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966169
更新日期：1980-10-01 00:00:00
abstract：:The hypothalamic paraventricular nucleus (PVN) is a key site for regulating neuroendocrine functions in the magnocellular part and autonomic activities in the parvocellular part. Its anatomical proximity to the third ventricle could be a good target for intrathecal injection of baclofen. We investigated the correlatio...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0621-0
更新日期：2012-02-01 00:00:00
abstract：:The neurotoxic effects of aluminum are generally associated with reduced antioxidant capacity, increased oxidative stress and apoptosis, which lead to the induction of neurodegenerative processes. Curcumin has a lipophilic polyphenol character and effects of antioxidant and anti-apoptotic. The present study was undert...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02839-9
更新日期：2019-08-01 00:00:00
abstract：:Tyrosinemia type II, also known as Richner-Hanhart syndrome, is an autosomal recessive inborn error of metabolism caused by a deficiency of hepatic cytosolic tyrosine aminotransferase, and is associated with neurologic and development difficulties in numerous patients. Considering that the mechanisms underlying the ne...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-013-1180-3
更新日期：2013-12-01 00:00:00
abstract：:In vitro aggregation and fibrillization of synthetic amyloid beta-protein Abeta 1-40 was assessed in the conditioned media from rhabdomyosarcoma (CRL 1598, HTB 82, HTB 153, CCL 136), adenocarcinoma (CCL 218), neuroblastoma (SY5Y), and COS cells cultured in the absence and presence of 10% heat-inactivated fetal bovine ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1027379926976
更新日期：1997-02-01 00:00:00
abstract：:Trans-caryophyllene (TC), a component of essential oil found in many flowering plants, has shown its neuroprotective effects in various neurological disorders. However, the effects of TC on epilepsy haven't been reported before. In this study, we investigated the effect of TC on kainic acid-induced seizure activity ca...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-014-1474-0
更新日期：2015-01-01 00:00:00
abstract：:The monoclonal antibody 14E immunocytochemically stains the nuclear membrane of oligodendrocytes but not myelin in tissue sections of adult normal human white matter. The nuclear membranes of Schwann cells in human peripheral nerve and cerebellar Bergmann glia were also visualized with this antibody. In actively demye...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00993270
更新日期：1992-09-01 00:00:00
abstract：:Nitric oxide has been strongly implicated in the development of tissue infarction in response to focal cerebral ischemia. Nitric oxide and its derivatives can inhibit components of the electron transport chain, providing a likely target for these substances in ischemic and post-ischemic brain. Lactate content is incre...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-006-9160-5
更新日期：2007-04-01 00:00:00
abstract：:A set of synaptic membrane antigens has been investigated in a number of tissues by indirect immunofluorescence histochemistry, using an antiserum (SPM-I) raised against a purified synaptosomal plasma membrane fraction prepared from day-old chick forebrain. The antigens were found to be present in both the central and...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/BF00966022
更新日期：1977-02-01 00:00:00
abstract：:Most studies of Alzheimer's disease (AD) have focused on a single precipitating alteration as the etiological event rather than global changes closely linked to aging. Recent evidence suggests that the most significant of these global changes are metabolic. Here we present data indicating that metabolic rate, nutritio...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1023/a:1025678510480
更新日期：2003-10-01 00:00:00
abstract：:The microdialysis technique was used to study the effect of nitric oxide synthase (NOS) activity on taurine release. Taurine release was characterized in rat striatum that was excitotoxically lesioned compared to normal conditions. The basal taurine level of the dialysate decreased during quinolinate (QUIN) lesion in ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022418231496
更新日期：1998-04-01 00:00:00
abstract：:We have investigated gene expression changes produced by acute and chronic daily treatment with a prototypical antidepressant, imipramine, using DNA microarrays. The analysis of similarities in gene expression patterns among functionally related genes revealed four expression profile cluster areas that showed a highly...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/b:nere.0000023610.89631.d6
更新日期：2004-06-01 00:00:00
abstract：:The binding of [3H]ethylketocyclazocine ([3H]EKC) under the suppression of mu and delta sites in the brain of EL mice (seizure-susceptible) was examined to determine the relationship between seizures and the dynorphinergic system. The density of kappa-opioid receptors in the cerebrum of adult EL mice during interictal...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1022424707543
更新日期：1998-02-01 00:00:00
abstract：:Unrelated cells such as dental follicle precursor cells (DFPCs) and retinal Müller cells (MCs) make spheres after cultivation in serum-replacement medium (SRM). Until today, the relation and molecular processes of sphere formation from different cell types remain undescribed. Thus in this study we compared proteomes o...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-011-0524-0
更新日期：2011-11-01 00:00:00
abstract：:The characteristics and properties of the increase in cytosolic [Ca2+] that occurs in bovine adrenal medullary chromaffin cells on exposure to angiotensin 11 have been investigated. In fura-2 loaded cells exposure to a maximally effective concentration of angiotensin II (100 nM) caused a rapid, but transient increase ...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1023/a:1024987128090
更新日期：2003-09-01 00:00:00
abstract：:It has been proposed that taurine may function as an oxidant in a dose-dependent manner in vivo and in vitro. The present study was carried out to investigate the relationship between taurine concentration and 3-nitrotyrosine level, a stable marker of peroxynitrite action, in hepatocytes of guinea pig in endotoxemia b...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-007-9395-9
更新日期：2007-11-01 00:00:00
abstract：:Docosahexaenoic acid (DHA) can alleviate cerebral ischemia/reperfusion injury by reducing blood-brain barrier permeability and maintaining its integrity, accompanied by an increased Ang-1/Ang-2 ratio; however, the underlying mechanisms of these effects remain unclear. Src-suppressed C kinase substrates (SSeCKS), a sub...
journal_title：Neurochemical research
pub_type： 杂志文章
doi：10.1007/s11064-019-02915-0
更新日期：2020-02-01 00:00:00
abstract：:Acetate is a two carbon intermediate in metabolism. It is an accepted marker of astrocytic metabolism, and a substrate for production of metabolites such as glutamine, glutamate and GABA. However, anomalies exist in the current explanations of compartmentation and metabolism of acetate. Here, we investigated these ano...
journal_title：Neurochemical research
pub_type： 杂志文章,评审
doi：10.1007/s11064-012-0847-5
更新日期：2012-11-01 00:00:00
