Abstract：
:The bidirectional transport of cargos along the thin axon is fundamental for the structure, function and survival of neurons. Defective axonal transport has been linked to the mechanism of neurodegenerative diseases. In this paper, we study the effect of the local axonal environment to cargo transport behavior in neurons. Using dual-color fluorescence imaging in microfluidic neuronal devices, we quantify the transport dynamics of cargos when crossing stationary organelles such as non-moving endosomes and stationary mitochondria in the axon. We show that the axonal cargos tend to slow down, or pause transiently within the vicinity of stationary organelles. The slow-down effect is observed in both retrograde and anterograde transport directions of three different cargos (TrkA, lysosomes and TrkB). Our results agree with the hypothesis that bulky axonal structures can pose as steric hindrance for axonal transport. However, the results do not rule out the possibility that cellular mechanisms causing stationary organelles are also responsible for the delay in moving cargos at the same locations.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Che DL,Chowdary PD,Cui Bdoi
10.1016/j.neulet.2015.10.066subject
Has Abstractpub_date
2016-01-01 00:00:00pages
110-6eissn
0304-3940issn
1872-7972pii
S0304-3940(15)30228-7journal_volume
610pub_type
杂志文章abstract：:In non-diabetic mice, s.c. injection of formalin to the hindpaw had a biphasic effect: an immediate nociceptive response (first-phase) followed by a tonic response (second-phase). However, only the immediate nociceptive response was observed in diabetic mice. The duration of the first-phase response was significantly ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(93)90761-9
更新日期：1993-01-12 00:00:00
abstract：:Previous studies using short-interval paired-pulse TMS have provided valuable insights into physiology of human motor cortex. Depending on the interstimulus interval (ISI) between the two pulses intra-cortical facilitation (ICF) or intra-cortical inhibition (ICI) can be observed. Similar patterns of inhibition and fac...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.03.036
更新日期：2005-07-15 00:00:00
abstract：:The putative anti-addiction alkaloid ibogaine and its principal metabolite 12-hydroxyibogamine appear to act at the (+)-5 methyl-10,11,dihydro-5H- dibenzo[a,d]cycloheten-5-10-imine maleate (MK-801) binding site in the N-methyl-D-aspartate (NMDA)-receptor cation channel. This conclusion is based on findings that both c...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11608-y
更新日期：1995-06-02 00:00:00
abstract：:Activation of several immediate early genes (IEGs) is crucial for long-term memory formation in vivo. In vitro methods of inducing these genes have not been investigated extensively. Here we present data demonstrating that application of the neurotrophin, nerve growth factor (NGF), to both rat primary neuronal culture...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.04.089
更新日期：2004-08-05 00:00:00
abstract：:Cultures were produced from retinas taken from human foetuses. Specific cells in culture have the capacity to take up exogenous serotonin, which was then visualized by immunofluorescence using an antisera to serotonin. The known serotonergic uptake blocker, chlorimipramine, inhibited the uptake of the amine while benz...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(85)90025-4
更新日期：1985-04-09 00:00:00
abstract：:The contribution of the circadian pacemaker and the sleep homeostat to sleep tendency and consolidation was quantified by forced desynchrony of the sleep-wake cycle from the circadian pacemaker in eight men who lived in time-isolation for 33-36 days. Analysis of 175 polygraphically recorded sleep episodes revealed tha...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90841-9
更新日期：1994-01-17 00:00:00
abstract：:Topographical maps form the basis of the organization in many projections within the central nervous system, but in the neuromuscular system such detailed spatial organization has generally been assumed to be absent and indeed unnecessary for normal function (see, for example, ref. 1). However, there is some physiolog...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(85)90598-1
更新日期：1985-10-10 00:00:00
abstract：:Activation of spinal astrocytes may contribute to neuropathic pain. Adjacent astrocytes can make direct communication through gap junctions formed by connexin 43 (Cx43) in the central nervous system. Yet, the role of spinal astroglial gap junctions in neuropathic pain is not fully understood. Since Cx43 is the connexi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.03.004
更新日期：2014-04-30 00:00:00
abstract：:Several clinical and epidemiological studies suggest that Alzheimer's disease (AD) and vascular dementia share common risk factors. Oxidative stress is one of these well recognized factors. It can result from an excess of free-radical activity and impaired antioxidant defenses. Paraoxonase (PON1), a component of high ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2003.12.100
更新日期：2004-03-18 00:00:00
abstract：:Recent evidence has been presented demonstrating that group III mechanoreceptors comprise an important part of the sensory arm of the exercise pressor reflex, which in turn functions to increase arterial blood flow to contracting skeletal muscles. Although group III afferents are stimulated by mechanical distortion of...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2013.09.013
更新日期：2013-10-25 00:00:00
abstract：:Female sex hormone, progesterone, in addition to seizure modifying activity is also known as a potential protective agent against various brain injury conditions. Considering the predisposal role of traumatic brain injury (TBI) on developing post-traumatic epilepsy (PTE), the effect of progesterone on post-traumatic e...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2019.134384
更新日期：2019-09-14 00:00:00
abstract：:Expression by rat motoneurons of mRNAs for the alpha subunit of the ciliary neurotrophic factor receptor (CNTFR alpha), receptor tyrosine kinase-C (trkC), calcitonin gene-related peptide (CGRP) and galanin was studied by in situ hybridisation, 6d after rat facial nerve transection on postnatal day 1. Similar numbers o...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(96)13124-4
更新日期：1996-11-08 00:00:00
abstract：:The cerebellar Purkinje cell is a major site for neurosteroid formation. We have demonstrated recently that the Purkinje cell actively produces sex steroids, such as estradiol and progesterone, de novo from cholesterol only during rat neonatal life, when cerebellar cortical formation occurs. We have further demonstrat...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.04.023
更新日期：2004-07-01 00:00:00
abstract：:The dorsal visual processing stream that includes V1, motion sensitive area V5 and the posterior parietal lobe, supports visually guided motor function. Two recent studies have reported associations between global motion perception, a behavioural measure of processing in V5, and motor function in pre-school and school...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2017.08.062
更新日期：2017-09-29 00:00:00
abstract：:The 22Na+ efflux stimulated by selected agonists of the 4 excitatory amino acid receptors, previously detected in the striatum, has been studied on 22Na+-preloaded slices prepared from 10 major areas of the rat brain. All brain areas were found to be sensitive, albeit to varying extents, to excitatory amino acids. The...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(83)90468-8
更新日期：1983-11-11 00:00:00
abstract：:In the present study we sought to investigate the signal transduction mechanisms that underlie the alpha2-adrenergic receptor (AR)-induced, subtype-specific neuronal differentiation of PC12 cells. Alpha2-ARs induced NF-kappaB transcriptional activity and p21(waf-1) gene transcription in the same subtype-specific manne...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2006.03.066
更新日期：2006-07-24 00:00:00
abstract：:The functional contribution of cloned subunits to insect nicotinic acetylcholine (ACh) receptors has been difficult to determine using heterologous expression. Instead, in this study we explore the subunit composition of naturally expressed functional receptors in an insect using RNA interference. The nicotinic alpha ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(01)02228-5
更新日期：2001-11-09 00:00:00
abstract：:Influences of a submaximal endurance test in the right first dorsal interosseus on force and fatigue-related parameters of activating the contralateral muscle were studied. The test consisted of a 30% maximum voluntary contraction (MVC), regularly interrupted by maximal contractions and brief rest periods. Despite the...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(98)00609-0
更新日期：1998-08-28 00:00:00
abstract：:The mechanism underlying the over-recovery of an M-type potassium current following the washout of muscarine (20 microM) has been examined. Whole-cell recordings were made from single neurons dissociated from bullfrog sympathetic ganglia. During over-recovery, the maximum M-conductance decreased by about 2.8 nS while ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)12495-2
更新日期：1996-03-29 00:00:00
abstract：:The endosome/lysosome system in the nervous system is critically important for a variety of neuronal functions such as neurite outgrowth, retrograde transport, and synaptic plasticity. In neurons, the endosome/lysosome system is crucial for the activity-dependent internalization of membrane proteins and contributes to...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2015.07.021
更新日期：2015-09-25 00:00:00
abstract：:The taiep rat is a myelin mutant whose immobility episodes (IEs) can be caused by gripping them by the tail. Electroencephalographic recordings during IEs show a rapid-eye movement sleep-like pattern, similar to narcolepsy-cataplexy in canines. Systemic administration of alpha(2)-adrenoceptor agonists and the alpha(1)...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.07.086
更新日期：2010-10-15 00:00:00
abstract：:Aggression reflects the psychological and physical behavior that perpetrator intends to harm victim. Initiation of aggression is influenced by the distal factors (e.g. personality) and proximate causes (e.g. affect) of perpetrator. However, few studies explored the brain structural basis of relationship between these ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2019.134728
更新日期：2020-01-23 00:00:00
abstract：:Twenty years ago, Wolfe, Xia, and Selkoe identified two aspartate residues in Alzheimer's presenilin protein that constitute the active site of the γ-secretase complex. Mutations in the genes encoding amyloid precursor protein (APP) or presenilin (PS) cause early onset familial Alzheimer's disease (AD), and sequential...
journal_title：Neuroscience letters
pub_type： 杂志文章,评审
doi：10.1016/j.neulet.2019.02.011
更新日期：2019-05-14 00:00:00
abstract：:Elongation factor-2 kinase (eEF-2K) is a Ca(2+)/calmodulin-dependent protein kinase that is highly specific for eukaryotic elongation factor-2. In the present study, we examined the developmental expression of eEF-2K messenger RNA (mRNA) in the mouse brain. At E13, the expression of eEF-2K mRNA is already evident in b...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00715-2
更新日期：2002-09-13 00:00:00
abstract：:Our aim was to investigate the neuromodulatory role of diadenosine tetraphosphate (Ap(4)A). Ap(4)A-binding sites were detected in striatum and hippocampus membranes using [(35)S]-ADP beta S as radioligand and Ap(4)A and epsilon-(Ap(4)A), di-ethenoadenosine tetraphosphate, as displacers. Effects of epsilon-(Ap(4)A) on ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(01)02067-5
更新日期：2001-08-31 00:00:00
abstract：:Increasing evidence suggests a relation between vascular disorders and late-onset Alzheimer's disease (AD). We performed an association analysis of low-density lipoprotein receptor-related protein (LRP), lipoprotein lipase (LPL), and angiotensin converting enzyme (ACE) genes, known to be involved in vascular disorders...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)01467-1
更新日期：2000-10-13 00:00:00
abstract：:Neuroglobin (Ngb) is a novel vertebrate globin expressed principally in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons against these insults. The mechanism of Ngb's protective action is unknown, but its ability to bind NO suggests that NO scavenging might be involved. To test this...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2007.10.031
更新日期：2008-01-10 00:00:00
abstract：:In recent years, animal behavioral models, particularly those used in pain research, have been increasingly scrutinized and criticized for their role in the poor translation of novel pharmacotherapies for chronic pain. This article addresses the use of animal models of pain from the perspective of industrial drug disc...
journal_title：Neuroscience letters
pub_type： 杂志文章,评审
doi：10.1016/j.neulet.2013.08.033
更新日期：2013-12-17 00:00:00
abstract：:The formation of the beta pleated configuration of the amyloid peptide fragment 25-35 in aqueous solution, has been studied using thioflavin-T fluorescence as an indicator of such folding. Both phosphate and adenosine triphosphate (ATP) enhance the formation of aggregated beta-sheets. This phosphate-induced aggregatio...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00307-9
更新日期：1999-05-21 00:00:00
abstract：:We previously reported that corticotropin-releasing factor (CRF) increased neuronal excitability specifically in type II neurons of the dorsolateral part of the bed nucleus of the stria terminalis (dlBNST). Because the majority of type II dlBNST neurons are thought to be GABAergic interneurons, at least a portion of w...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2015.05.059
更新日期：2015-07-23 00:00:00
